A layer of polyaniline conductive material was formed on the surface of nylon fabric by low-temperature in situ polymerization, and its morphology and chemical composition were characterized by field emission scanning electron microscopy (FESEM) and micro-Raman imaging spectroscopy. The conductivity of polyaniline nylon fabric was tested by the multimeter and electrochemical workstation. The results showed that polyaniline polymerized on the surface of the nylon fabric imparted better electrical conductivity to the fabric. The electrical conductivity of the fabric was 31.62 S/m.The resistance of polyaniline nylon fabric increases with the increase of strain. when the strain returns to the initial state, the fabric resistance recovers gradually and approaches to the initial value. The polyaniline fabric still has a high cycle stability of 96.2% after 3500 stretching-releasing cycles.As a conductive fabric, polyaniline nylon fabric has good sensing performance, which can accurately monitor the joint movement of the human body in strain range from 0% to 15%. Meanwhile, polyaniline nylon fabric has thermoelectric properties, and the Seebeck coefficient of the fabric is 8.406 μV/K, which is expected to be used as a temperature difference sensor.