Bacillus and its extracellular metabolites are used in skin care products due to their antioxidant and antibacterial properties. The active substances were extracted from the fermentation product of Bacillus ZYCHH-01 by hydrochloric acid precipitation method, and the in vitro antioxidant activity of the extract was evaluated by using the free radical scavenging rate as an index, the antibacterial ability of the extract was assessed by Oxford Cup diffusion test, and the biosafety of the extract-added cream was examined by acute skin irritation and human application tests. The results showed that the extract exhibited some scavenging activity against DPPH- and ABTS+- with semi-clearance (IC50) of 0.73 and 0.26 g/L, respectively. The extract inhibited both Gram-positive and negative bacteria and could cause severe damage to the bacterial morphology. In the Chinese cosmetic industry, the maximum amount of single nipagin ester used is 0.4wt%, and the maximum amount of mixed ester used is 0.8wt% of the overall. When the mass concentration of the extract is 8 g/L (0.8wt%), it can effectively inhibit the growth of six common pathogenic bacteria and has the feasibility of replacing commercially available preservatives; and the prepared cream has safety and no skin irritation.