Sapindus mukorossi Gaertn extracting solution as a natural active raw material to care products, in order to promote the application of Sapindus mukorossi Gaertn extracting solution in daily chemical industry, the water extraction was purified by microbial fermentation method. Lactobacillus plantarum was selected as the suitable fermentation strain based on sugar consumption and saponin purity, Sapindus mukorossi fermented liquid (SF) was prepared. The anti-acne effect of SF was studied from the three major causes of acne: sebum, inflammation and overgrowth of pathogenic bacteria. The results showed that 20 μg/mL SF reduced the lipid binding rate of SZ95 cells by 26.4%; the levels of NO, TNF-α, IL-6 and ROS in RAW 264.7 cells induced by lipolyaccharide (LPS) were decreased by 52.3, 57.5, 56.2 and 35.9%, respectively; The MIC of SF to Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus were 3.125, 12 .5 and 6.25 g/L, respectively, by double dilution method. Through the human efficacy evaluation, the facial acne situation was significantly improved.