CO₂-responsive monomer N, N-Dimethyl oleic amide propyl tertiary amine (DOAPA) with tertiary amine structure was prepared by using 3-dimethylaminopropylamine and oleic acid as main agents, and was intermixed with organic counter ions to construct CO₂-responsive worm-like micelle (CO₂-TWMS). The response of the system was characterized by the changes of conductivity, chemical structure and micro-morphology before and after contact with CO₂. The anti-channeling performance and mechanism of the system were revealed by combining the changes of surface activity of the system and the changes of pressure and recovery efficiency in the process of CO₂ displacement in dense matrix/crack medium and CO₂-TWMS channeling prevention. The experimental results show that the viscosity of CO₂-TWMS system is the highest when the inverse ion is sodium p-toluene sulfonate and the molar ratio of DOAPA is 1:1. CO₂ and N₂ can stimulate the reversible change of electrical conductivity between 0.9ms/cm and 1.95ms/cm, and induce the transformation of micromorphology between wormlike micelles and spherical micelles. In addition, CO₂ can reduce the critical micelle concentration from 1mmol/L to 0.25mmol/L, and the lowest surface tension from 30.2mN/m to 29.1mN/m. The adsorption efficiency and efficacy of the system at the gas-liquid interface are enhanced, which is conducive to the formation of micelles. In the dense matter-fracture medium, CO₂ induced wormlike micelle formation, increased the pressure difference during displacement, expanded the CO₂ displacement sweep efficiency, and increased the recovery rate by 22.6%.