In order to solve the problems of serious destruction of secondary structure, low molecular weight and long extraction time of regenerated keratin extracted by traditional reduction method, chicken feather was dissolved and keratin was extracted and regenerated by deep eutectic solvent (DES). In this study, different types of DES were used as solvents. By comparing the extraction rate and molecular weight of regenerated keratin extracted from them, DES with less damage to diagon protein and higher extraction rate was screened. The extraction process was optimized from three aspects: reducing agent, temperature and water content in DES. The hydrogen bond network structure in DES solvent was analyzed by density functional (DFT) theory and solvent color parameters, and the weak loss dissolution mechanism of chicken feather keratin was studied. Finally, the optimal extraction process is as follows: ethanolamine: choline chloride = 2:1 (molar ratio) water bath heating to obtain DES, the ratio of solid to liquid is 1: When the oil bath is heated at 30,65 ℃ for half an hour, the extraction rate of regenerated keratin is 31.8%, and the molecular weight is up to 66KDa, which is 100% higher than the molecular weight of 33KDa of regenerated keratin extracted by traditional reduction method.