In this study, a stable acrylate copolymer latex has been prepared through emulsion polymerization using styrene, butyl methacrylate and methyl methacrylate as main monomers, beheneth-25 methacrylate (BEM) with big hydrophilic and hydrophobic groups simultaneously as functional monomer. Subsequently, the self-matting coating was obtained from the acrylic latex. When the amount of BEM is controlled at 15% (based on the total mass of main monomers of St, BA and MMA), the effects of emulsifier kinds and dosage, and the buffer adding method on the polymerization stability and the monomer conversion were studied. When the weight ratio of emulsifiers is constant, and the buffer of NaHCO3 was all added with the initiator solution simultaneously, the influence of the BEM dosage (0%、5%、10%、15%、20%) on the glossiness and transmittance of coatings were investigated. In addition, the composition and morphology of coatings were characterized by FTIR、SEM、AFM. By using a comprehensive emulsifier system to form hydrophobic interaction between emulsifiers and BEM, and adding the buffer and initiator simultaneously, the stability of polymerization and the monomer conversion were improved while the coagulation is lower than 0.38% and the monomer conversion is higher than 98%. The self-matting performance of coatings could be enhanced due to the construction of micro-rough surface induced by the self-migration of the big alkyl groups from the inner matrix to the coating surface. When the amount of BEM is 20% (based on the total mass of main monomers), the coating has high transmittance (≥90%) and low glossiness (35.4%), indicating the balance between the matting performance and the transparency of coatings.