A new type of copper-dopped carbon dots (CuQACDs) with photothermal/ photodynamic synergistic antibacterial ability was prepared via a one-step hydrothermal method using the aqueous solution of CuCl2 and allyl trimethyl ammonium chloride. The CuQACDs was characterized by TEM, FTIR, XPS, Raman, and UV. The obtained CuQACDs were well-dispersed spherical nanoparticles, with the average particle size of 4.9 nm and the zeta potential of +8.17 mV. CuQACDs had a photothermal conversion efficiency of 21.7% and could produce ROS in bacterial cells while being irradiated by a single laser of 808 nm at 1.5 W/cm2. CuQACDs expressed excellent antibacterial capability to E. coli. And S. aureus. The cell viability of E.coli and S.aureus was1.67% and1.06%, respectively after irradiation for 10 min with 808n m laser at1.5 W/cm2. SEM images showed that the surface of bacteria crumpled and ruptured after laser irradiation, leading to bacterial death. This study supplies a highly efficient nanocarbon antibacterial material and multimodal antibacterial method for killing pathogenic bacteria and healing the wounds.