In this study, WVOx/SiO2 catalysts (CatT, T=350, 400, 450, 500, 600), prepared through impregnation method using industrial mesoporous SiO2 as support, were used in gas-glycerol catalytic conversion. The BET, XRD, NH3-TPD, and XPS results showed that CatT have a well-developed mesoporous structure (d0 5.1-6.8 nm) with specific surface area of 215-235 m2/g. The calcination temperature effectively turned and adjusted the distribution, chemical composition, and acidic strength of WVOx on the surface of SiO2. At 300 ℃, the gas-glycerol conversion over Cat400 reached 89.3%, with the selectivity of acrolein and acetaldehyde reached 53.4% and 22.1%, respectively, and the carbon balance of liquid condensation products reaches 86%. Cat400 also showed good regeneration ability.