In order to improve the additional value of waste distiller"s resource utilization and achieve efficient adsorption and degradation of dyes, the microbial immobilized porous charcoal (Massibacillus-DGPC) for the removal of methylene blue (MB) from dyeing wastewater was prepared by using Bacillus massiliogabonensis strain and porous charcoal material (DGPC). The FTIR, EDS and SEM were used to characterize The structure of Massibacillus-DGPC. The environmental factors such as pH and salinity on Massibacillus-DGPC adsorption and degradation methylene blue were studied. The results showed that though the adsorption process of Massibacillus-DGPC was affected by many factors, The decolorization rate can maintain more than 78% in different pH and salinity environments. In addition, compared with other treatment groups, Massibacillus-DGPC showed superior adsorption and degradation capacity and renewable capacity. The MB removal rate of Massibacillus-DGPC reached more than 99% in 6 rounds of recycling. After 8 rounds of recycling, the MB removal rate of Massibacillus-DGPC was still as high as more than 92%, and achieved The complete degradation of more than 50% MB. The microbe immobilized porous distiller"s grains charcoal has good adsorption and degradation performance of MB, and has strong renewable ability. The results have certain value for the efficient treatment of dye wastewater by recycling of distiller"s grains waste.