In order to study the optimal extraction process, physicochemical properties and antioxidant and hypoglycemic activities of Conioselinum vaginatium,ultrasound-assisted extraction of Conioselinum vaginatium was carried out using 1-ethyl-3methylimidazole bromide (ionic liquid), and the optimal extraction process of polysaccharides was optimized by single-factor and response surface experiments. The crude polysaccharide (CV-1) of Conioselinum vaginatium after removing impurities such as protein, pigment and small molecules, was subjected to preliminary physicochemical property studies.The in vitro antioxidant capacity of CV-1 was evaluated by ABTS radical scavenging and hydroxyl radical scavenging, and the in vitro hypoglycemic capacity of CV-1 was evaluated by α-glucosidase and α-amylase inhibition. The results showed that the optimal extraction process of CV-1 was as follows: sonication time of 115 min, enzyme digestion time of 90 min, sonication temperature of 55 ℃, ionic liquid mass concentration of 4 g/L, material-liquid ratio of 1:50, and mass fraction of pectinase of 0.6 %, and the amount of CV-1 extracted under these conditions was (125.278 ± 0.707 mg/g).The weighted relative molecular mass of CV-1 was 17.57 CV-1 has a gravimetric relative molecular mass of 17.57 KDa, and is mainly composed of five sugar residues (mannose, glucose, rhamnose, arabinose, fucose, molar percentages of 5.94 %, 6.8 %, 28.03 %, 18.4 %, 40.83 %), is a triple-helical conformational α-constituent glycoside coexisting in an amorphous and crystalline structure, and does not contain proteins and glucuronic acid, and has an excellent thermal stability, and has an excellent thermal stability.Scanning electron microscopy revealed that CV-1 has a lamellar structure with locally flat surfaces.The half-scavenging concentrations (IC50) for ABTS radical and hydroxyl radical were 1.122 mg/mL and 1.373 mg/mL, respectively, and the IC50 for α-glucosidase and α-amylase were 14.799 mg/mL and 15.739 mg/mL.
Keywords:Conioselinum vaginatium polysaccharide; chemicalcomposition; physico chemical; antio-xidant activity; hypoglycemic activity