Enzyme immobilization is a promising approach for the industrial application of enzymes, and the design of the carrier is a key factor. Metal-organic frameworks (MOFs) is highly ordered porous materials with clear structure-function relationships, uniform pore aperture distribution, tunable and designable porosity at the atomic level. There have been significant advances in the research of MOFs as enzyme carriers in the past ten years, and the related methods and applications have been systematically reviewed previously. However, few review focus on the influence of porosity on property of immobilized enzyme. Thus, this review focuses on the systematic introduction of the influence of pore structure on the properties of immobilized enzyme. Firstly, the formation mechanism of MOFs pores is analyzed, followed by a review of the progress in loading enzymes in cage-type cavities and channel-type pores, as well as the influence of porosity on catalysis process. As prospect, the advantages of hierarchical porosity in substrate diffusion and the importance of developing porosity - enzyme matched MOFs are discussed firstly. Then, the advantages of fabricating MOFs-Enzyme cascade catalyzer with minimized diffusion resistance within confinement under nanoscale,and the enhancement of enzymatic catalysis through microenvironment adjustment are analyzed as well.