To evaluate the effect of origin on Tartary buckwheat"s active ingredients, antioxidant capacity, and hypoglycemic activity, Tartary buckwheat in seven different production areas as the object of study, firstly, to determine its active ingredients (total polyphenols, total flavonoids, and D-chiral myo-inositol), antioxidant capacity (DPPH free radicals, ABTS + radical scavenging rate) and hypoglycemic activity (inhibition of α-glucosidase and α-amylase), and then by principal component analysis and cluster analysis for multi-factor statistical analysis. The results showed that the active ingredients, antioxidant capacity, and hypoglycemic activity of Tartary buckwheat from different production areas have significant differences. Tartary buckwheat (DT) in North China, had the highest total polyphenol [in gallic acid (GAE)] and D-chiral inositol (DCI) content, respectively, 37.24±0.48 mg (GAE)/g and 3.97±0.32 mg (DCI)/g; total polysaccharide content [in glucose (DE)] was the lowest, 14.42±0.38 mg (DE)/g; on the DPPH radicals and ABTS + radical scavenging ability is the strongest, half scavenging mass concentration of 0.630±0.075 and 0.119±0.008 g/L; α-glucosidase and α-amylase half of the inhibition of the mass concentration of 1.228±0.056 and 1.327±0.146 g/L. A kind of Tartary buckwheat (SQ) in central China, the total flavonoids [as rutin ( RE)] content was the highest, 15.47±0.19 mg (RE)/g. A buckwheat (QJ) in Southwest China had the highest content of total polysaccharides, 24.98±0.75 mg (DE)/g. The antioxidant capacity and hypoglycemic activity of the active ingredients are total polyphenols, total flavonoids, and D-chiral inositol.