For the purposes of improve the functionality of cotton fabric, cotton fabrics were modified with glyoxal and chitosan (CS) to obtain CS-modified cotton fabrics, then the CS modified cotton fabric was impregnated with graphene oxide (GO) dispersion, and finally the GO adsorbed on the fabric was reduced to reduced graphene oxide (RGO). RGO/CS-cotton fabric with conductive, UV resistant and antibacterial properties was prepared. Based on SEM, FTIR and Raman spectroscopy, the effects of CS mass fraction and GO dispersion mass concentration on the electrical conductivity, UV resistance and antibacterial properties of RGO/CS-cotton fabric were investigated, and the washability of the fabric was tested. The results show that the GO deposited on the surface of RGO/ CS-cotton fabric is fully reduced to RGO, and the surface is covered with a continuous layer of RGO/CS film. The electrical conductivity of RGO/CS-cotton fabric increases gradually with the increase of GO dispersion concentration, mainly due to the good electrical conductivity of RGO. The RGO/CS-cotton fabric modified with 2% CS solution and treated with 5 g/L GO dispersion has the best electrical conductivity, and the surface resistance is 1.02 kΩ/cm. The antibacterial performance of RGO/CS-cotton fabric was enhanced by the collaboration of RGO and CS, and the antibacterial rate was up to 95%. The RGO/CS-cotton fabric modified with 0.5% CS solution and treated with 3 g/L GO dispersion after 30~50 times of soaping has little change in conductivity, bacteriostatic and UV resistance.