Using lingonberries ethanol extract and Lonicera japonica polyphenols as raw materials, a long-wave ultraviolet (UVA) induced oxidative damage model of Human hepatocellular carcinomas (HepG2 cells) was established to investigate the protective effect of Lonicera japonica polyphenols and lingonberries ethanol extract complex on UVA irradiated HepG2 cells and related enzyme activities, This includes the determination of superoxide dismutase (SOD) activity, catalase (CAT) activity, malondialdehyde (MDA) concentration, and changes in reactive oxygen species (ROS) content after being irradiated with half lethal dose of UVA. The experiment showed that the survival rates of HepG2 cells pretreated with a 1:1 mixture of Lonicera japonica polyphenols and Lonicera japonica ethanol extract in low, medium, and high dose groups increased by 15.26%, 33.59%, and 66.14% compared to the model group, respectively; SOD activity increased by 10.72%, 32.43%, and 54.08%; CAT activity increased by 16.48%, 36.9%, and 116.2%; The activity of glutathione peroxidase (GSH-Px) increased by 52.97%, 71.44%, and 117.14%; MDA concentration decreased by 17.12%, 31.22%, 59.41%. and ROS expression level significantly decreased. Research has confirmed that the 1:1 complex of ethanol extract from lingonberrie with Lonicera japonica polyphenols can alleviate oxidative stress damage in HepG2 cells induced by UVA irradiation by activating the antioxidant enzyme system and clearing ROS. This study lays the foundation for the research on sun protection and radiation resistance of lingonberries ethanol extract and Lonicera japonica polyphenols.