Abstract:Mitochondria are a major source of cellular reactive sulfur species (RSS). The RSS play crucial roles in physiological and pathological processes of life. In this paper, we review the recent research status of organic one and two-photon fluorescent probes for RSS in mitochondria, such as H2S, SO2, biothiols, H2Sn , etc. The properties of fluorescent probe selectivity, sensitivity, recognition mechanism, cell toxicity and biological monitoring imaging are emphatically discussed. Moreover, the existing problems and future development of this kind of fluorescent probes are proposed．This review is significantly meaningful in theory and practice for the construction of novel mitochondrial fluorescent probes and further dissection of the cellular function of RSS.

Abstract:A Schiff-based fluorescent molecular probe (HSZn) was synthesized by condensation of quinoline with phenylhydrazine and characterized by FTIR, NMR and HRMS. The UV-Vis spectroscopy and fluorescence spectroscopy studies demonstrated that the probe showed a significant fluorescence enhancement effect toward Zn2+ in aqueous medium while suffering from a slight interference at the presence of other metal ions. A good linear relationship between the Zn2+ concentrations (1.0×10-5~1.0×10-4 mol/L) and the fluorescence intensity was obtained for determining the content of Zn2+ with a detection limit of 4.0×10-8 mol/L. A 1:2 binding ratio of Zn2+/HSZn for the complex was obtained by Job’s plot and LC-MS, HSZn also exhibited a sensitive fluorescence response toward Zn2+ in the wide pH range (pH=5~13). Cell imaging studies indicated that the probe was capable of detecting endogenous Zn2+ in live Hela cells.

Abstract:Aniline, p-methoxy aniline, chloroacetyl chloride as materials, the amide was introduced into intermediates molecular structure by amidation. Two series of surfactants(Ⅰ, Ⅱ) were synthesized by intermediates with N, N-dimethyldecylamine, N, N-dimethyldodecylamine, N, N-dimethyltetradecylamine by quaternization respectively. The structures were characterized by 1H-NMR and IR spectra. The critical micelle concentrations (CMC), foaming, foam stability and emulsification ability of Ⅰ and Ⅱ were determined at 25℃. CMC minimum value of Ⅱ14 was 0.50 mmol/L, the best foam stability of Ⅰ14 and Ⅱ14 was 100%, the longest emulsification time of Ⅰ12 was 1602 s. Under the same conditions, for BAC-12, the CMC was 9.51 mmol/L, foam stability was 44%, the emulsification time was 366 s. The results of the tests illustrate that Ⅰ and Ⅱ showed better surface activity and better foaming, foam stability, emulsifying properties.

Abstract:A composite structure of magnetic nano photosensitizer TiO2/Fe3O4-SiO2 has been prepared by precipitation, Stöber and sol-gel methods, using ferrous sulfate, ferric chloride, tetraethyl orthosilicate and titanium tetrachloride as raw materials. Structure and property of the nano composite photosensitizer were characterized and analyzed by means of XRD, TEM, UV-Vis DRS and magnetic test. Methyl thiazolyl tetrazolium colorimetric method (MTT method) was used to investigate the killing effect of nano composite photosensitizer on A549 human lung cancer cells in vitro, and effects of different TiO2 loadings of TiO2/Fe3O4-SiO2 on the inhibition ratios of A549 human lung cancer cells were also studied. The characterization results showed that the nano composite photosensitizer had core shell structure and magnetism, had visible light absorption capability, and could respond to visible light at wavelengths above 400 nm. In vitro cytotoxicity assay results showed that when the illumination time was 150 min, mole fractions of inhibiting proliferation of A549 human lung cancer cells for the nano composite photosensitizer and pure TiO2 were 51% and 36% respectively, the nano composite photosensitizer with enhanced photodynamic activity. The optimum load for TiO2 was 30% in mass fraction.

Abstract:The aggregation behavior of N-Dodecyl-N, N-Dimethy–3–Ammonio–1– Propanesulfonate(SB12-3)/sodium dodecyl benzene sulfonate(SDBS) surfactant mixed system at oil/water interface was studied by molecular dynamics simulation. The effects of Ca2 on the amphipathic nature of SB12-3/SDBS surfactant mixed system were investigated at the microscopic point of view. The simulation results showed that SDBS and SB12-3 exhibited the best synergistic effects as the proportion of them reached 4:6. Ca2 replaced the position of Na at the oil/water interface, and compressed the headgroups of SB12-3 and SDBS so that both the surfactants were arranged more closely at oil/water interface. Radial distribution function indicated that the interaction between -SO3- in SB12-3 and SDBS and water molecules increased with the addition of Ca2 , Na were closer to -SO3- in SB12-3, and the thickness of electric double layer was compressed. The solvent accessible surface areas also declined obviously when Ca2 existed in the solution. By calculating order parameter and angles between the hydrophilic groups and hydrocarbon chains of SDBS and SB12-3, it was found that Ca2 could enhance the interaction between decane and hydrocarbon chains of SB12-3 and SDBS, and reduce the oil/water interfacial tension.

Abstract:Using Butyl methacrylate(BMA), 2-ethylhexyl methacrylate(2-EHM) and styrene(St) as raw material, polyvinyl alcohol(PVA) as dispersant agent, benzoyl peroxide(BPO) as initiator, divinylbenzene(DVB) as crosslinking agent, modified attapulgite as inorganic additive to prepare high oil-absorption resin with microwave irradiation. The major influences, including mass fraction of initiator，styrene, OATP and reaction temperature were studied. Meanwhile, the structure, thermal performance and surface topography of oil-absorption resins were analyzed by FTIR, TGA and SEM, respectively. The result shows: when w(St)=52.54%、m(2-EHM) : m(BMA)=1:1.5、w(BPO)=1.95％、w(DVB)=0.6％、w(PVA)=3％、w(OATP)=2.0％，at 82℃,reaction time is 70min, microwave power is 700W, it has the maximum oil absorbency to carbon tetrachloride, dichloromethane, toluene and xylene. That is 37.24, 30.50, 16.94 and 16.81g/g, respectively, the thermal decomposition temperature of the resin was improved 32℃ after adding OATP into the resin.

Abstract:The fermentation conditions of sophorolipids (SLs) by Candida bombicola O-13-1 with waste fried oil (WFO) as lipid substrates were optimized through orthogonal experiment, and the structures of individual SL components were quantified and identified by HPLC-MS/MS. The results showed that the optimized conditions in shake-flask were determined as follows: agitation 200r/min, carbon source added amount 90g/L, temperature 25℃, inoculum size 12% (volume fraction). The highest yield of SLs of 57.64g/L was obtained at the end of the stationary phase of yeast cell growth, i.e. 204h. The products were composed of 9 kinds of acetylated lactonized and acidic SLs, and the lactonic forms accounted for up to 76% (mass fraction). Moreover, the yield of SLs was as high as 163.28g/L in a 5L automatic fermentor under the optimized conditions of shake-flask with the exception of agitating of 500r/min. The material cost of SLs fermentation with WFO as carbon source reduced by 18% compared with soybean oil, and the resource utilization of WFO could be realized while effectively reducing the production cost of SLs.

Abstract:Abstract: Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was investigated. With L-glutamlhydrine and propylamine as substrate, the factors such as substrate concentration, pH and temperature were investigated. The results indicated that when the concentration of L-glutamlhydrine was 0.4 mol/L, the concentration of propylamine was 3 mol/L, reaction temperature was 37ºC, and pH was 10, the yield of obtained propyl-L-glutamine reached 97.9%.The yield of propyl-L-glutamine was 85.3%. The purity of propyl-L-glutamine was 99.8%. Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was effective.

Abstract:Porous carbon supported Cu2O/Cu dual-phase catalysts were prepared via a combined solvothermal and sintering method, and their catalytic activities were investigated in catalytic nitrophenol reduction. XRD characterization indicated that the Cu2O/Cu phase can be obtained by sintering Cu-BTC under air atmosphere, whereas Cu phase can be gotten under nitrogen annealing atmosphere. The morphologies of catalysts Cu2O/Cu were checked using SEM and TEM. SEM and TEM images showed that Cu2O/Cu composite catalyst obtained in the nitrogen atmosphere has smaller particle size and higher dispersions than those of CuO catalyst obtained under air atmosphere. In the catalytic nitrophenol reduction, porous carbon supported Cu2O/Cu dual-phase catalysts obtained under nitrogen atmosphere exhibits much higher catalytic activity compared with CuO from air atmosphere.

Abstract:Four kinds of commercially ultra-high-temperature skimmed milk selected as the research object, the flavor attributes of samples evaluated by sensory descriptive analysis and electronic nose technique. Component analysis (PCA), cluster analysis (CA) and partial least squares regression (PLSR) used to analyze the correlation between electronic nose sensor performance and sensory attributes. The results obtained in the study showed that creamy and fat attributes had low sensory score. Ten sensors of electronic nose had good ability to differ four samples, the correlation of sensory attributes and electronic sensors explained by PLSR model that reflects the overall information reflected. Sensory evaluation combined with electronic nose to analyze four UHT skim milk, we can test the overall information by electronic nose in a rapid way and analyze samples by sensory evaluation specifically. Combined these two methods can make up the defects of sensory evaluation and intelligence sensory technology and available to provide a reference of improving the flavor of UHT skimmed milk.

Abstract:In order to study and develop the indole quinazoline alkaloid of the traditional Chinese medicine evodia, the thiorutaecarpine and five N-substituted derivatives were synthesized from rutaecarpine by thionation. The structures of the products were confirmed by HPLC-HRMS, IR, 1HNMR and 13CNMR. The antifungal activity on nine plant pathogens of the compounds (Ⅱ and Ⅳa~e, 10μg/mL) was analyzed, and it was found that there was almost no inhibitory effect on the plant pathogenic fungi, and N-substituted thiorutaecarpine (Ⅳa, Ⅳb and Ⅳd) had obvious antifungal activity against bacteria [Xanthomonas oryzae pv.oryzae (Ishiyama)]. The inhibitory rates of N-benzyl sulfo-rutaecarpine (Ⅳb) and N-(4-methyl benzyl) sulfo-rutaecarpine (Ⅳd) were 81.84% and 59.95%, respectively. The rutaecarpine derivatives were found to be almost non-toxic (IC50 > 80 μmol/L) to human normal cells (HFL1) by cytotoxicity assay.

Abstract:A kind of kind of benzoic acid derivatives influenza neuraminidase inhibitors I to IV were prepared from the starting materials of 4-amino-3-iodine benzoic acid through a series of reactions , the compounds V - VII derivatived from compound II , and the structures were characterized by 1H NMR,13C NMR and MS.Inhibition rate determined by chemical fluorescence, and the activity test was performed by the influenza virus strains H3N2 (California / 04), H1N1 (California / 99) and B (Shanghai / 02), the results showed: Among the compounds I - IV, the greater side chain at C3 position,the lower activity of compounds; Among the compounds V - VII, introduction of hydroxyl on the α carbon in the side chains of benzene ring, the inhibitory activity stronger than single hydroxyl on β carbon, which suggested that the change of electronic environment on the α carbon had a greater influence on activity; Compound VIIcontaining single hydroxyl at α carbon in side chains of benzene ring possessed the strongest activity of type A influenza virus.

Abstract:The carbon black -polyurethane (CB-PU) particle with core-shell structure was prepared by the core material (CB) and shell material (PU) the in-situ emulsion synthesis method, The structure and the properties of the CB before and after modified were analyzed by FTIR,TG ,SEM and EDS; The dispersion test of the particles showed that the dispersion and stability of CB-PU in water were significantly improved than that of CB; Then the CB before and after modified was ultrasound dispersed in waterborne polyurethane (WPU) coatings to prepare conductive coatings with different CB content (CB/WPU and CB-PU/WPU), Finally, coating and drying the coating to prepare the conductive membrane. The study showed that the CB-PU can be uniformly dispersed in WPU coating films due to the hydrophilicity of the polyurethane which were completely coated on the surface of CB. The homogeneous dispersion of CB-PU greatly increased the density and intensity of the conductive network of conductive particles in coatings, which leads to a remarkable increase in the conductivity of the coating. In addition, when the volume resistivity was main-tained about 3.3×103 Ω•cm, the mass fraction of CB could dropped from 12wt %( in the CB/WPU film) to 6wt %( in the CB-PU/WPU film). The result indicates that it is an effective method to improve the dispersity of CB in the matrix and promote the formation of the conductive network by coating the matrix resin layer on the surface of CB. The mechanical test showed that the breaking elongation of the CB-PU/WPU was better than that of CB/WPU, while the breaking tenacity decreased.

Abstract:In view of the disadvantages of high cost、hash experimental conditions and low utilization rate of raw materials in existing reports. In the paper, 3,4-dihydroxybenzaldehyde was used as raw material, refer to the literature method, and β-(3,4-dihydroxyphenyl) pyruvate borneol ester was synthesized at first, then under the bread’s yeast fermentation liquid and target product Danshensu borneol ester was synthesized. The influence of amount of glucose and β-cyclodextrin、reaction time 、pH were investigated in the paper. When β-(3,4-dihydroxyphenyl) pyruvate borneol ester was 0.5g , And the best conditions were as follow: amount of glucose was 30g , reaction time was 24h, pH was 7, amount of β-cyclodextrin was 1.5g. enantiomeric excess valve of the target product as high as 84.9%,and main configuration was S-DBZ . The reaction conditions of the process in this paper were mild and the cost was low, which was a very valuable process for the production of Danshensu borneol ester.

Abstract:A series of dipyrromethanes was synthesized by the tandem reaction of pyrrole and aldehyde in 40% alcohol solution at 50 oC using α-chymotrypsin as biocatalyst. This method have the merits of mild reaction conditions, shorter reaction time, broad substrate scope and no need of nitrogen protection. The results further expand the application of enzyme’s promiscuity and α-chymotrypsin in the organic synthesis.

Abstract:2,3,5,6-tetraaminopyrazine (TAPA) was synthesized from iminodiacetonitrile through nitrosation, cyclization, nitration and reduction. The structure of the target product as well as that of the intermediates of each reaction was confirmed by NMR, IR, ESI-MS. The overall yield was 26.8%. The effects of different cyclization reaction temperatures and nitration systems on the yields of intermediates were investigated respectively. In addition, the influence factors of hydrogenation reduction reaction were discussed. The optimal conditions were determined as follows: The temperature of cyclization reaction was 25℃;In the nitration reaction, the best system of nitration was selected as KNO3 and fuming H2SO4; In the reduction reaction, the mass ratio of Pd/C to 2,6-diamino-3,5-dinitropyrazine-1-oxide was 1:15, the best solvent system was selected as CH3OH and H2O (volume ratio 1:1), the reaction temperature was 55℃ and hydrogen pressure was in 0.8～1.2MPa . The advantages of this new synthetic route were that the starting material was commercially available and that the intermediates had good stability, which can reduce the cost of synthesis greatly.

Abstract:4-Allyloxymethyl-1,3-dioxolan-2-one (AGC) was prepared by the coupling reaction of allyl glycidyl ether (AGE) and CO2 using 3-bromo-1,1,1-trifluoro-2-propanol (BTFP) as activator. The coupling reaction was catalyzed by TBAB organocatalyst. Many kinds of quaternary ammonium salts and activators were screened. The effects of reaction temperature, activator amount, carbon dioxide pressure and reaction time on the yield of the product were investigated. A possible reaction mechanism was proposed. The product was analyzed by gas chromatography coupled with mass spectrometry (GC-MS), infrared spectroscopy (FTIR) and - nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR). The results demonstrated that the yield of the corresponding cyclic carbonate could reach 96.5% when BTFP was 2 mol% of AGE, n-butylammonium bromide (TBAB) was 2 mol% of AGE at 0. 5 MPa CO2, 90 ℃ for 18 h under a solvent-free condition.