Page 210 - 201905
P. 210

·978·                             精细化工   FINE CHEMICALS                                  第 36 卷

                 图 12 中可以得出,当吸附操作温度在 293.15 K                      and  high-performance  liquid  chromatography[J].  Journal  of
                                                                   Chromatography A, 2000, 891(1): 115-127.
                                                 –1
            时,吸附总传质系数 K F ·a v 可达到 0.158 s ,此时传                [9]   Sanz  N  V,  Benavente  F,  Toro  I,  et al.  Optimization  of  HPLC
                                                                   conditions for the separation of complex crude mixtures produced in
            质区长度 Z a 降到最低 3.7 cm。从介质吸附量以及传                         the synthesis of the therapeutic peptide homones[J]. Chromatographia,
            质效果分析得出,最佳吸附操作温度为 293.15  K,                           2001, 53(1): S167-S173.
                                                               [10]  Lozinsky V I. Polymeric cryogels as a new family of macroporous
            即室温下具有较好吸附效果。                                          and  supermacroporous  materials  for  biotechnological  purposes[J].
                                                                   Russian Chemical Bulletin, 2008, 57(5): 1015-1032.
                 综上所述,最佳的动态吸附条件为:鱼皮多肽                          [11]  Zhan X Y, Lu D P, Lin D Q, et al. Preparation and characterization of
            吸附液流速为 3~4  mL/min ,吸附 操作温度为                           supermacroporous  polyacrylamide  cryogel  beads  for  biotechnological
                                                                   application[J].  Journal  of  Applied  Polymer  Science,  2013,  130(5):
            293.15 K,鱼皮多肽进料液质量浓度 400~500 mg/L。                     3082-3089.
                                                               [12]  Mendis  E,  Rajapakse  N,  Kim  S  K.  Antioxidant  properties  of  a
            在此条件下的动态吸附量为 74.5~79.4 mg/g。                           radical-scavenging peptide purified from enzymatically prepared fish
                                                                   skin  gelatin  hydrolysate[J].  Journal  of  Agricultural  and  Food
                                                                   Chemistry, 2005, 53(3): 581-587.
            3    结论                                            [13]  Mendis  E,  Rajapakse  N,  Byun  H  G,  et al.  Investigation  of  jumbo
                                                                   squid  (Dosidicus  gigas)  skin  gelatin  peptides  for  their  in vitro
                                                                   antioxidant effects [J]. Life Sciences, 2005, 77(17): 2166-2178.
                (1)采用抗坏血酸代替传统胺类还原剂在低温                          [14]  Aevidsson  P,  Plieva  F  M,  Savina  I  N,  et al.  Chromatography  of
            环境中通过一步法制得富含—COOH 和—NH 2 两性                            microbial  cells  using  continuous  supermacroporous  affinity  and
                                                                   ion-exchange  columns[J].  Journal  of  Chromatography  A,  2002,
            功能基团的规整吸附介质 p(AA-co-CTS),摒弃了现                          977(1): 27-38.
                                                               [15]  Aevidsson P, Plieva F M, Lozinsky V I, et al. Direct chromatographic
            有主流技术中通过后期改性或者化学修饰使介质获                                 capture of enzyme from crude homogenate using immobilized metal
                                                                   affinity  chromatography  on  a  continuous  supermacroporous
            得功能基团的方法,聚合时间缩短至 5 h,以期在海                              adsorbent[J]. Journal of Chromatography A, 2003, 986(2): 275-290.
            洋活性多肽提取技术上规模化推广与应用。                                [16]  Persson P, Baybak O, Plieva F, et al. Characterization of a continuous
                                                                   supermacroporous monolithic matrix for chromatographic separation
                (2)p(AA-co-CTS)最佳聚合配方为:引发剂总                        of  large  bioparticles[J].  Biotechnology  and  Bioengineering,  2010,
                                                                   88(2): 224-236.
            质量分数为 0.75%,V C 与 APS 的质量比为 0.5∶1.0,               [17]  Plieva  F  M,  Andersson  J,  Galaev  I  Y,  et al.  Characterization  of
            单体总质量分数为 7%,CTS 与 AA 的质量比为 0.2∶                        polyacrylamide based monolithic columns[J]. Journal of Separation
                                                                   Science, 2004, 27(10/11): 828-836.
            1.0,交联剂质量分数为 1.05%。                                [18]  Aevidsson P, Plieva F M, Lozinsky V I, et al. Direct chromatography
                                                                   capture of enzyme from crude homogenate using immobilized metal
                (3)建立鱼皮多肽在 p(AA-co-CTS)介质上动态                       affinity  chromatography  on  a  continuous  supermacroporous
                                                                   adsorbent[J]. Journal of Chromatography A, 2003, 986(2): 275-290.
            吸附行为的传质方法,通过传质单元数法分析总传                             [19]  Yao K J, Shen S C, Yun J X, et al. Preparation of polyacrylamide-
            质系数、传质区长度等参数与吸附操作的关系,判                                 based  supermacroporous  monolithic  cryogel  beds  under  freezing-
                                                                   temperature  variation  conditions[J].  Chemical  Engineering  Science,
            定吸附属于液相扩散控制过程。                                         2006, 61(20): 6701-6708.
                                                               [20]  Yao  K  J,  Yun  J  X,  Shen  S  C,  et al.  Protein  adsorption  in
                (4)传质单元数法分析得出最佳吸附工艺为:                              supermacroporous  cryogels  with  embedded  nanoparticles  [J].
            吸附液流速为 3~4 mL/min,吸附温度为 293.15 K,                      Biochemical Engineering Journal, 2007, 36(2): 139-146.
                                                               [21]  Liu Jie (刘杰), Shen Shaochuan (沈绍传), Chen Ping (陈平), et al.
            进料液质量浓度 400~500 mg/L,最大动态吸附容量                          Separation  of  cytidine  triphosphate  from  Saccharomyces  cerevisiae
                                                                   broth  by  anion  exchange  poly  (2-hydroxyethyl  methacrylate)
            Q m =74.5~79.4 mg/g。                                   composite  cryogel  embedded  with  SiO 2  nanoparticles  [J].  CIESC
                                                                   Journal (化工学报), 2014, 65(10): 3938-3944.
            参考文献:                                              [22]  Guo Yantao (郭延涛), Shen Shaochuan (沈绍传), Yun Junxian (贠军
                                                                   贤 ),  et al.  Chromatographic  separation  of  plasmid  DNA  by
            [1]   Zhuang Yongliang (庄永亮). Study on the biochemical characterization   anion-exchange cryogel [J]. Chinese Journal of Biotechnology (生物
                 of collagen and skin care activity of collagen peptides extracted from   工程学报), 2012, 28(8): 995-1001.
                 jellyfish  rhopilema  esculentum[D].  Qingdao:  Ocean  University  of   [23]  Chen F, Yao K J, Yun J X, et al. Influence of grafting conditions on
                 China (中国海洋大学), 2009.                             the properties of polyacrylamide-based cation-exchange cryogels grafted
            [2]   Rushikesh S, Pravin P, Seetharama J. Peptides, peptidomimetics, and   with  2-acrylamido-2-methyl-1-propanesulfonic  acid[J].  Chemical
                 polypeptides  from  marine  sources:  A  wealth  of  natural  sources  for   Engineering Science, 2008,(63): 71-77.
                 pharmaceutical applications[J]. Marine Drugs, 2017, 15(124): 1-37.     [24]  Yun J X, Jepspersen G R, Kirsebom H, et al. An improved capillary
            [3]   Velusamy A, Manigandan V, Saravanan R. et al. Bioactive peptides   model  for  describing  the  microstructure  characteristics,  fluid
                 from  marine  ascidians  and  future  drug  development–A  review[J].   hydrodynamics and breakthrough performance of proteins in cryogel
                 International Journal of Peptide Research and Therapeutics, 2018, 24,   beds[J]. Journal of Chromatography A, 2011, 1218 (32): 5487-5497.
                 (1): 13-18.                                   [25]  Ye Zhenhua (叶振华). Chemical adsorption separation process (化工
            [4]   Yang  H,  Wang  Y,  Zhou  P,  et al.  Effects  of  alkaline  and  acid   吸附分离过程) [M]. Beijing: China Petrochemical Press (中国石化
                 peteatment  on  the  physical  properties  and  nanostructures  of  the   出版社), 1992: 131-134.
                 gelatin  from  channel  catfish  skins[J].  Food  Hydrocolloide,  2008,   [26]  Kitagawa Hiroshi〔(日)北川浩〕, Akihiro Suzuki〔(日)铃木谦一郎〕.
                 22(8): 1541-1550.                                 Fundamentals  and  design  of  adsorption  (吸附的基础与设计),  Lu
            [5]   Fang  W  Y,  Dahiya  R,  Qin  H  L,  et al.  Natural  proline-rich   Zhengli〔鹿政理(译)〕[M]. Beijing: Chemical Industry Press(化学工
                 cyclopolypeptides  from  marine  organisms:  Chemistry,  synthetic   业出版社), 1983: 79-110.
                 methodologies  and  biological  status[J].  Marine  Drugs,  2016,   [27]  He  Zhao  ( 何钊 ), Ren Qilong ( 任其龙 ).  Separation  of
                 14(194): 1-22.                                    proanthocyanidin  from  extracts  of  grape  seeds  by  chromatographe
            [6]   Daniela G, Maria C, Daniela C, et al. Biotechnological applications   [J]. Food Science (食品科学), 2004, 25(8): 70-72.
                 of bioactive peptides from marine sources[J]. Advances in Microbial   [28]  Zhao  Ping  (赵平),  Zhang  Yueping  (张月萍), Ren Peng  (任鹏).
                 Physiology, 2018, 73: 171-220.                    Adsorption  process  of  proanthocyanidins  in  AB-8  macroporous
            [7]   Assaad  S,  Ali  B.  Antioxidant  peptides  from  marine  by-products:   resin[J]. CIESC Jorunal (化工学报), 2013, 64(3): 980-985.
                 isolation, identification and application in food systems. A review[J].   [29]  National  Technical  Committee  for  Standardization  of  Special  Diet
                 Foods, 2016, 21(3): 10-26.                        (全国特殊膳食标准化技术委员会). GB/T22729—2008:Oligopeptides
            [8]   Dan N, Ganesan R, Flood K G, et al. Determination of enantiomers   powder  of  marine  fish  (海洋鱼低聚肽粉)[S].Standards  Press  of
                 in a synthetic argininal peptide using capillary zone electrophoresis   China(中国标准出版社), 2008: 1-10.
   205   206   207   208   209   210   211   212   213   214   215