Page 49 - 《精细化工》2022年第9期

第 39 卷第 9 期 精 细 化 工 Vol.39, No.9
2022 年 9 月 FINE CHEMICALS Sept. 2022

综论
荧光传导机理构建 β-半乳糖苷酶探针的研究进展

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李 苗 ，马秀琴 ，金潇仪 ，杨 帆 ，田 野
（1. 大连工业大学 生物工程学院，辽宁 大连 116034；2. 大连海洋大学 海洋科技与环境学院，辽宁 大
连 116023）
摘要：β-半乳糖苷酶（β-Gal）是细胞溶酶体中的一种糖苷水解酶，在催化糖苷键水解以及维持正常生命活动等
领域起着至关重要的作用。开发能精确、快速检测 β-Gal 活性的方法对于卵巢癌、细胞衰老引发疾病的早期诊
断和分子生物学领域的研究是十分重要的。考虑到荧光探针具有检测灵敏、响应迅速和时空分辨率高等特性，
该综述对近年来报道的 β-Gal 荧光探针研究进展及其疾病诊断效果进行了总结，介绍了依据分子内电荷转移、
荧光共振能量转移、聚集诱导发光和光诱导电子转移等信号传导机理构建 β-Gal 荧光探针的分子设计策略及应
用特点，并重点介绍了二维检测体系能够突破探针使用单一信号传导机理进行检测时常遇到的局限。指出合理
应用荧光信号传导机理构建具有灵敏性、空间分辨率高，化学稳定性好和细胞蓄积能力强等特点的 β-Gal 荧光
探针，能够推进 β-Gal 荧光探针在卵巢癌诊断及治疗效果评价，细胞衰老程度评估和个体健康寿命预测等生物
领域的高效应用。
关键词：荧光探针；β-半乳糖苷酶；卵巢癌；细胞衰老
中图分类号：O657.3；Q503 文献标识码：A 文章编号：1003-5214 (2022) 09-1767-14

Research progresses on β-galactosidase probes based on
fluorescence signal transduction mechanisms

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LI Miao , MA Xiuqin , JIN Xiaoyi , YANG Fan , TIAN Ye
（1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China; 2. College of
Marine Technology and Environment, Dalian Ocean University, Dalian 116023, Liaoning, China）
Abstract: β-Galactosidase (β-Gal), a glycoside hydrolase in cellular lysosomes, plays a crucial role in
hydrolyzing glycoside bonds and even maintaining normal life activities. Development of rapid and
accurate identification methods for β-Gal activity is of great importance for the early diagnosis of ovarian
cancer and diseases triggered by cellular senescence as well as researches in molecular biology. Fluorescent
probes show advantages of sensitive detection, rapid response, and high temporal-spatial resolution.
Therefore, the research progresses on β-Gal fluorescence probes reported in recent years and their effects on
disease diagnosis was firstly summarized, followed by introduction of the molecular design strategies and
application characteristics of β-Gal fluorescence probes based on intramolecular charge transfer,
fluorescence resonance energy transfer, aggregation-induced emission, and photoinduced electron transfer
fluorescence-signal transduction mechanisms, especially the two-dimensional detection system which could
break through the limitation of single signal transduction mechanism for probe detection. Finally, it was
pointed out that development of β-Gal fluorescent probes with high sensitivity, spatial resolution, good
chemical stability and strong cell accumulation capacity based on fluorescence signal transduction
mechanism could advance the highly efficient applications of β-Gal fluorescent probes in biomedical fields,
such as diagnosis and therapeutic evaluation of ovarian cancer, cellular senescence assessment, and
individual life-span prediction.

收稿日期：2022-05-11; 定用日期：2022-07-13; DOI: 10.13550/j.jxhg.20220456
基金项目：国家自然科学基金（22108024，31901775）；辽宁省自然科学基金（2021-BS-226）；大连市青年科技之星项目（2021RQ113）；
设施渔业教育部重点实验室（大连海洋大学）资助项目（202224）
作者简介：李 苗（1991—），女，讲师，E-mail：limiao@dlpu.edu.cn。联系人：田 野（1988—），男，副教授，E-mail：tianye@dlou.edu.cn。