Page 170 - 《精细化工》2023年第1期
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第 40 卷第 1 期                             精   细   化   工                                  Vol.40, No.1
             20 23 年 1 月                             FINE CHEMICALS                                  Jan.  2023


              水处理技术与环境保护
                           微生物燃料电池-好氧颗粒污泥系统中


                                      C/N 对阴极微生物的影响



                                                                                  *
                                      谢岳林,程   洁,王子骏,欧阳二明
                                       (南昌大学  资源与环境学院,江西  南昌  330100)

                 摘要:为了探索微生物燃料电池(MFC)更广泛的适用性,将好氧颗粒污泥(AGS)与 MFC 进行耦合,并采
                                                                               +
                 用序批式运行方式,固定进水化学需氧量(COD)为 780 mg/L,通过改变 NH 4 -N 的质量浓度(39、50、78 和
                                      +
                 156 mg/L)将 COD 与 NH 4 -N 的质量浓度之比(简称碳氮比,C/N)分别调节为 20、15.6、10、5,研究了不同
                 C/N 对系统阴极室内微生物的多样性和菌群结构产生的影响。通过高通量测序分析显示,随着进水 C/N 的变化,
                 阴极室内不同菌群的相对丰度都产生了明显的变化。在门水平下,当 C/N=10 时,最为丰富的变形菌门
                 (Proteobacteria)相对丰度为 45.0%,而当 C/N=20 时降为 41.1%。在纲水平下,当 C/N=5 时,最为丰富的异常球
                 菌纲(Deinococci)相对丰度均为 27.2%,而当 C/N=20 时只有 15.1%。当 C/N=15.6 和 20 时,阴极室内微生物的
                 新陈代谢最高,相对丰度均为 77.1%,对应的阴极好氧颗粒污泥代谢能力较强,对微生物降解 COD 具有重要作用。
                 关键词:微生物燃料电池;好氧颗粒污泥;碳氮比;高通量测序;微生物群落;水处理技术
                 中图分类号:TM911.45       文献标识码:A      文章编号:1003-5214 (2023) 01-0162-07


                         Effect of C/N ratio on cathodic microorganisms in microbial

                                     fuel cell-aerobic granular sludge system

                                                                                      *
                                  XIE Yuelin, CHENG Jie, WANG Zijun, OUYANG Erming
                        (College of Resources and Environment, Nanchang University, Nanchang 330100, Jiangxi, China)


                 Abstract: In order to explore its wider applicability, microbial fuel cell (MFC) was coupled with aerobic
                 granular sludge (AGS) via adopting sequencing batch operation. Then, the effects of C/N ratio on microbial
                 diversity and community structure in the cathode chamber of the system was studied by fixing the influent
                                                                                          +
                 chemical oxygen demand (COD) at 780 mg/L and adjusting the mass concentration of NH 4 -N to 39, 50, 78
                                                                         +
                 and 156 mg/L to keep the mass concentration ratio of COD and NH 4 -N (carbon to nitrogen ratio for short,
                 C/N) to  20,  15.6, 10 and  5, respectively.  High-throughput sequencing  analysis showed that the relative
                 abundance of different bacterial communities in the cathode chamber significantly varied with the change of
                 influent C/N.  The  relative abundance of  Proteobacteria  was  decreased from 45.0% to 41.1% as  C/N
                 changed from 10 to 20 at the phylum level, while that of Deinococci reduced from 27.2% to 15.1% as C/N
                 increased from 5 to 20. When C/N=15.6 and 20, the metabolic relative abundance of microorganisms in the
                 cathode chamber reached the highest (77.1%) and led to strong metabolism of the corresponding cathode
                 aerobic granular sludge, which played an important role in the microbial degradation of COD.
                 Key words:  microbial fuel cell; aerobic granular sludge; carbon and nitrogen  ratio; high-throughput
                 sequencing; microbial community; water treatment technology


                                                                                                   [1]
                 微生物燃料电池(MFC)是一种生物电化学装                         有机基质中的化学能转化为清洁的电能 ,在修复
            置,其利用微生物的代谢活动来实现能量转换,将                             水体污染问题的同时可在一定程度上缓解能源危


                 收稿日期:2022-06-25;  定用日期:2022-08-19; DOI: 10.13550/j.jxhg.20220593
                 基金项目:江西省自然科学基金面上项目(20192BAB206039)
                 作者简介:谢岳林(1998—),男,硕士生,E-mail:1520081828@qq.com。联系人:欧阳二明(1976—),男,副教授,硕士生导师,
                 E-mail:youmer@ncu.edu.cn。
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