Page 170 - 《精细化工》2023年第1期
P. 170
第 40 卷第 1 期 精 细 化 工 Vol.40, No.1
20 23 年 1 月 FINE CHEMICALS Jan. 2023
水处理技术与环境保护
微生物燃料电池-好氧颗粒污泥系统中
C/N 对阴极微生物的影响
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谢岳林,程 洁,王子骏,欧阳二明
(南昌大学 资源与环境学院,江西 南昌 330100)
摘要:为了探索微生物燃料电池(MFC)更广泛的适用性,将好氧颗粒污泥(AGS)与 MFC 进行耦合,并采
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用序批式运行方式,固定进水化学需氧量(COD)为 780 mg/L,通过改变 NH 4 -N 的质量浓度(39、50、78 和
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156 mg/L)将 COD 与 NH 4 -N 的质量浓度之比(简称碳氮比,C/N)分别调节为 20、15.6、10、5,研究了不同
C/N 对系统阴极室内微生物的多样性和菌群结构产生的影响。通过高通量测序分析显示,随着进水 C/N 的变化,
阴极室内不同菌群的相对丰度都产生了明显的变化。在门水平下,当 C/N=10 时,最为丰富的变形菌门
(Proteobacteria)相对丰度为 45.0%,而当 C/N=20 时降为 41.1%。在纲水平下,当 C/N=5 时,最为丰富的异常球
菌纲(Deinococci)相对丰度均为 27.2%,而当 C/N=20 时只有 15.1%。当 C/N=15.6 和 20 时,阴极室内微生物的
新陈代谢最高,相对丰度均为 77.1%,对应的阴极好氧颗粒污泥代谢能力较强,对微生物降解 COD 具有重要作用。
关键词:微生物燃料电池;好氧颗粒污泥;碳氮比;高通量测序;微生物群落;水处理技术
中图分类号:TM911.45 文献标识码:A 文章编号:1003-5214 (2023) 01-0162-07
Effect of C/N ratio on cathodic microorganisms in microbial
fuel cell-aerobic granular sludge system
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XIE Yuelin, CHENG Jie, WANG Zijun, OUYANG Erming
(College of Resources and Environment, Nanchang University, Nanchang 330100, Jiangxi, China)
Abstract: In order to explore its wider applicability, microbial fuel cell (MFC) was coupled with aerobic
granular sludge (AGS) via adopting sequencing batch operation. Then, the effects of C/N ratio on microbial
diversity and community structure in the cathode chamber of the system was studied by fixing the influent
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chemical oxygen demand (COD) at 780 mg/L and adjusting the mass concentration of NH 4 -N to 39, 50, 78
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and 156 mg/L to keep the mass concentration ratio of COD and NH 4 -N (carbon to nitrogen ratio for short,
C/N) to 20, 15.6, 10 and 5, respectively. High-throughput sequencing analysis showed that the relative
abundance of different bacterial communities in the cathode chamber significantly varied with the change of
influent C/N. The relative abundance of Proteobacteria was decreased from 45.0% to 41.1% as C/N
changed from 10 to 20 at the phylum level, while that of Deinococci reduced from 27.2% to 15.1% as C/N
increased from 5 to 20. When C/N=15.6 and 20, the metabolic relative abundance of microorganisms in the
cathode chamber reached the highest (77.1%) and led to strong metabolism of the corresponding cathode
aerobic granular sludge, which played an important role in the microbial degradation of COD.
Key words: microbial fuel cell; aerobic granular sludge; carbon and nitrogen ratio; high-throughput
sequencing; microbial community; water treatment technology
[1]
微生物燃料电池(MFC)是一种生物电化学装 有机基质中的化学能转化为清洁的电能 ,在修复
置,其利用微生物的代谢活动来实现能量转换,将 水体污染问题的同时可在一定程度上缓解能源危
收稿日期:2022-06-25; 定用日期:2022-08-19; DOI: 10.13550/j.jxhg.20220593
基金项目:江西省自然科学基金面上项目(20192BAB206039)
作者简介:谢岳林(1998—),男,硕士生,E-mail:1520081828@qq.com。联系人:欧阳二明(1976—),男,副教授,硕士生导师,
E-mail:youmer@ncu.edu.cn。