Page 112 - 《精细化工》2023年第8期

·1726· 精细化工 FINE CHEMICALS 第 40 卷

中固定化琼胶酶颗粒回收不完全，导致酶活降低； α-neoagarobiose hydrolase for enhancing the production of 3,6-
anhydro-L-galactose[J]. Journal of Agricultural and Food Chemistry,
另一方面是循环过程中酶从载体上脱落、失活或结 2018, 66(27): 7087-7095.
构变性等所致。如何改进固定过程，减少酶的脱落， [10] XIE W, HUANG M. Fabrication of immobilized Candida rugosa
lipase on magnetic Fe 3O 4-poly(glycidyl methacrylate-co-methacrylic
使琼胶酶的固定更牢固以及如何优化回收过程，减 acid) composite as an efficient and recyclable biocatalyst for enzymatic
少固定化酶颗粒的损失等，是今后研究的重点。 production of biodiesel[J]. Renewable Energy, 2020, 158: 474-486.
[11] GUO J M (郭姣梅), HAO J H (郝建华), LI X H (李晓涵), et al.
Preparation of aminosilanized magnetic nanoparticles and immobilization
3 结论 of cyclodextrin glucosyltransferase[J]. Journal of Functional Materials
(功能材料), 2020, 51(7): 7190-7195.
[12] BOURKAIB M C, GAUDIN P, VIBERT F, et al. APTES modified
（1）利用 APTES 对磁性复合材料进行功能化改
SBA15 and meso-macro silica materials for the immobilization of
性用于固定化琼胶酶。通过 SEM 观察到载体材料表 aminoacylases from Streptomyces ambofaciens[J]. Microporous and
Mesoporous Materials, 2021, 323: 111226.
面光滑，而固定化琼胶酶因酶的附着表面粗糙。固定 [13] CAO X, XU H, LI F, et al. One-step direct transesterification of wet
化琼胶酶的 FTIR 分 析表明，存在琼胶酶和 yeast for biodiesel production catalyzed by magnetic nanoparticle-
immobilized lipase[J]. Renewable Energy, 2021, 171: 11-21.
Fe 3O 4@SiO 2-NH 2 的特征峰。VSM 测定固定化琼胶酶 [14] FANG G, CHEN H, ZHANG Y, et al. Immobilization of pectinase
的磁化饱和强度为 42.8 emu/g，具有较高的磁响应性。 onto Fe 3O 4@SiO 2-NH 2 and its activity and stability[J]. International
Journal of Biological Macromolecules, 2016, 88: 189-195.
（2）通过条件实验得到优化的固定条件为：加 [15] MANSOUR A M, IBRAHIM R S, AZAB A A. Structure,
酶量为 7 mL，戊二醛加入量为 4 mL，交联时间 2 h， morphology, optical and magnetic studies of Fe 3O 4-doped CdS
nanocomposite[J]. Journal of Materials Science: Materials in
固定时间 2 h，在该条件下，酶活回收率为 67.74%。 Electronics, 2022,33:10251-10258.
（3）相比于游离琼胶酶，固定化形成的共价键 [16] ASLANI E, ABRI A, PAZHANG M. Immobilization of trypsin onto
Fe 3O 4@SiO 2-NH 2 and study of its activity and stability[J]. Colloids
以及载体材料表面特殊性质造就的良性微环境使得 and Surfaces B: Biointerfaces, 2018, 170: 553-562.
固定化琼胶酶的热稳定性和 pH 稳定性显著提高。 [17] MIAO C, YANG L, WANG Z, et al. Lipase immobilization on
amino-silane modified superparamagnetic Fe 3O 4 nanoparticles as
（4）固定化琼胶酶在第 7 次使用后相对活性为 biocatalyst for biodiesel production[J]. Fuel, 2018, 224: 774-782.
[18] BRADFORD M M. A rapid and sensitive method for the quantitation
40.41%，具有重复利用性。
of microgram quantities of protein utilizing the principle of protein-
基于氨基硅烷功能化磁性材料固定化琼胶酶是 dye binding[J]. Analytical Biochemistry, 1976, 72(1): 248-254.
[19] ZHANG X W (张曦文), WU L (伍菱), TONG F J (童风景), et al.
可行的，且具有较好的酶活回收率、热稳定性、pH Effects of storage temperature and humidity on enzyme activities and
稳定性及重复利用性等，可为今后工业化利用琼胶 strength of agar gel in brown alga gracilaria[J]. Fisheries Science (水
产科学), 2020, 39(6): 880-886.
酶提供一定的理论依据。 [20] LI C (李驰), LI C S (李春生), YANG X Q (杨贤庆), et al. Isolation
and identification of agarase-producing vibrio fluvialis for agarase
参考文献： preparation and purification[J]. Food and Fermentation Industries (食
品与发酵工业), 2020, 46(7): 35-42.
[1] XIANG Y (相悦), SUN C F (孙承锋), LI L H (李来好), et al. Effect [21] ARIAEENEJAD S, MOTAMEDI E, HOSSEINI S G.
of agaric oligosaccharides and EGCG on the freshness and protein Immobilization of enzyme cocktails on dopamine functionalized
biochemical properties of ice-frozen fillets of perch[J]. Journal of magnetic cellulose nanocrystals to enhance sugar bioconversion: A
Fisheries of China (水产学报), 2021,45(7): 1007-1020.
biomass reusing loop[J]. Carbohydrate Polymers, 2021, 256: 117511.
[2] ZHANG X, AWEYA J J, HUANG Z, et al. In vitro fermentation of [22] ZHANG Y, ZHU L, WU G, et al. A novel immobilized enzyme
gracilaria lemaneiformis sulfated polysaccharides and its agaro- enhances the conversion of phosphatidylserine in two-phase system[J].
oligosaccharides by human fecal inocula and its impact on microbiota[J]. Biochemical Engineering Journal, 2021, 172: 108035.
Carbohydrate Polymers, 2020, 234: 115894. [23] DE OLIVEIRA R L, DA SILVA M F, DA SILVA S P, et al.
[3] LI Q (李倩), YANG R (杨锐), CHEN H M (陈海敏), et al. Effect of Fructo-oligosaccharides production by an Aspergillus aculeatus
oligoagars on the growth and quality of okar[J]. Journal of Nuclear commercial enzyme preparation with fructosyltransferase activity
Agricultural Sciences (核农学报), 2019, 33(7): 1465-1471. covalently immobilized on Fe 3O 4-chitosan-magnetic nanoparticles[J].
[4] LIN Z K (林志魁), ZHAO S H (赵守辉), XU Z F (许紫芬), et al. International Journal of Biological Macromolecules, 2020, 150: 922-
Preparation of a new agar oligosaccharide facial mask essence[J]. 929.
Guangdong Chemical Industry (广东化工), 2022, 49(8): 77-80.
[5] LIU X (刘雪), CHEN Y H (陈艳红), JIANG Z D (姜泽东), et al. [24] LIAO J, HAN S, LI X, et al. Co-immobilization of two-component
hydroxylase monooxygenase by functionalized magnetic nanoparticles
Characterization of a neoagarobiose-producing and thermostable for preserving high catalytic activity and enhancing enzyme stabilty[J].
β-agarase from Agarivorans sp.AL1 and antioxidant activity of the International Journal of Biological Macromolecules, 2020, 164:
enzymatic hydrolysates[J]. Modern Food Science and Technology 3163-3170.
(现代食品科技), 2021, 37(5): 82-90. [25] WU E, LI Y, HUANG Q, et al. Laccase immobilization on amino-
[6] ZOU Y, FU X, LIU N, et al. The synergistic anti-inflammatory functionalized magnetic metal organic framework for phenolic compound
activities of agaro-oligosaccharides with different degrees of removal[J]. Chemosphere, 2019, 233: 327-335.
polymerization[J]. Journal of Applied Phycology, 2019, 31(4): 2547- [26] KHAN M, HUSAIN Q, BUSHRA R. Immobilization of β-
2558. galactosidase on surface modified cobalt/multiwalled carbon nanotube
[7] XIAO Q, LIU C, NI H, et al. β-Agarase immobilized on tannic nanocomposite improves enzyme stability and resistance to inhibitor[J].
acid-modified Fe 3O 4 nanoparticles for efficient preparation of bioactive International Journal of Biological Macromolecules, 2017, 105: 693-
neoagaro-oligosaccharide[J]. Food Chemistry, 2019, 272: 586-595.
701.
[8] ZHANG L, GUO X, SONG Y, et al. Bioadhesive immobilize [27] RODRIGUES R C, BERENGUER-MURCIA Á, CARBALLARES
agarase on magnetic ferriferous by polydopamine[J]. Materials D, et al. Stabilization of enzymes via immobilization: Multipoint
Science and Engineering: C, 2018, 93: 218-225. covalent attachment and other stabilization strategies[J]. Biotechnology
[9] WANG Q, SUN J, LIU Z, et al. Coimmobilization of β-agarase and
Advances, 2021, 52: 107821.