Page 120 - 《精细化工》2021年第7期

第 38 卷第 7 期 精 细 化 工 Vol.38, No.7
202 1 年 7 月 FINE CHEMICALS July 2021

生物工程
等离子体预处理对解淀粉芽孢杆菌生长和

α-淀粉酶分泌的影响

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纪 亮 ，汤羽萌 ，田嘉美 ，许洁婷 ，邵汉良 ，朱 劼 1,4*
〔1. 常州大学 药学院医学院（筹），江苏 常州 213164；2. 未米生物科技（江苏）有限公司，江苏 常
州 213032；3. 常州中科常泰等离子体科技有限公司，江苏 常州 213161；4. 生物质高效炼制及高质化
利用国家地方联合工程研究中心，江苏 常州 213164〕
摘要：采用低真空室温射频等离子体对解淀粉芽孢杆菌 CICC 10035 进行预处理，通过改变放电功率、处理时间
和工作气压等参数筛选工艺条件，并使用蛋白质组学技术对差异蛋白质进行生物信息学分析，探讨等离子体预
处理对菌体生长和产酶性能的影响。得到的等离子体预处理最佳工艺条件为：放电功率 120 W，预处理时间 15 s，
工作气压 135 Pa。在该条件下，发酵 48 h 后，α-淀粉酶酶活超过 400 U/mL，与对照样相比提高约 20%。蛋白组
学分析表明，等离子体预处理对菌体的蛋白合成及代谢产生了较强的正效应，尤其在 DNA 修复和氨基酸合成上，
一方面提高了菌体活性，延缓了其衰亡过程；另一方面促进了 α-淀粉酶在菌体内的合成和分泌。
关键词：低真空室温射频等离子体；解淀粉芽孢杆菌；蛋白组学；生物信息学；α-淀粉酶；生物工程
中图分类号：TQ925.1 文献标识码：A 文章编号：1003-5214 (2021) 07-1402-07

Effects of plasma pretreatment on the cell growth of

Bacillus amyloliquefaciens and α-amylase secretion

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JI Liang , TANG Yumeng , TIAN Jiamei , XU Jieting , SHAO Hanliang , ZHU Jie 1,4*
〔1. School of Pharmacy & School of Medicine, Changzhou University, Changzhou 213164, Jiangsu, China; 2. Wimi
Biotechnology (Jiangsu) Co., Ltd., Changzhou 213032, Jiangsu, China; 3. Changzhou Zhongke Changtai Plasma Technology
Co., Ltd., Changzhou 213161, Jiangsu, China; 4. National-Local Joint Engineering Research Center of Biomass Refining and
High-Quality Utilization, Changzhou 213164, Jiangsu, China〕
Abstract: Bacillus amyloliquefaciens CICC 10035 was pretreated by low vacuum and room temperature
plasma (LVRTP). The optimal process conditions were screened by changing parameters such as discharge
power, treatment time and working pressure. The differential proteins were analyzed by bioinformatics
using proteomics technology, and the effects of plasma pretreatment on the cell growth and α-amylase
secretion were discussed. The results showed that the optimal LVRTP pretreatment conditions were as
follows: discharge power of 120 W, treatment time of 15 s, working pressure of 135 Pa. Under these
conditions, α-amylase activity in the fermentation was more than 400 U/mL after 48 h, about 20% higher
than that of the control. Proteomic analysis indicated that LVRTP pretreatment had strong positive effects
on the protein synthesis and cell metabolism, especially in DNA repair and amino acid synthesis. LVRTP
pretreatment improved the activity of cells and delayed the aging process. In addition, it promoted the
synthesis and secretion of α-amylase in cells.
Key words: low vacuum and room temperature plasma; Bacillus amyloliquefaciens; proteomics;
bioinformatics; α-amylase; biological engineering

收稿日期：2021-01-16; 定用日期：2021-03-09; DOI: 10.13550/j.jxhg.20210060
作者简介：纪 亮（1995—），男，硕士生，E-mail：2568434112@qq.com。联系人：朱 劼（1977—），男，教授，硕士生导师，E-mail：
zhujie@cczu.edu.cn。