Page 167 - 《精细化工》2023年第6期
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第 6 期   陈秀琼，等:  基于互穿网络技术构筑的氧化海藻酸钠/纤维素纳米晶/聚丙烯酰胺-明胶双网络水凝胶与性能  ·1317·


            对比空白对照，OSA/CNCs/PAM-GT 体系的相对                       步提高了 167%（OSA/2.0% CNCs/PAM-GT 复合水凝
            ALP 活性更高，说明 CNCs 的掺入能够提高细胞的                        胶）。而且它们的溶胀率随 CNCs 含量增加呈下降趋
            分化能力。其中，当 CNCs 的含量为 1.5%时，细胞                       势而生物降解性呈上升趋势，但是它们的变化幅度
            的分化效果最好。之后再增加 CNCs 含量，细胞的                          较小，说明 CNCs 能够在一定程度上调控复合水凝
            相对 ALP 活性下降，这可能是由于随着 CNCs 含量                       胶的物化性能。细胞相容性实验结果表明，MG63
            的增大，导致复合水凝胶材料的孔隙率变小，从而                             细胞能够黏附在 OSA/CNCs/PAM-GT 复合水凝胶
            影响了细胞的分化。                                          上，同时能够进行细胞增殖和分化。当 CNCs 的含
                                                               量为 0.5%时，细胞增殖效果最佳，而 CNCs 的含量
                                                               为 1.5%时，细胞分化效果最显著。以上结果说明，
                                                               将 CNCs 掺杂到 OSA/PAM 互穿网络基体中能够有
                                                               效地提高 OSA/CNCs/PAM-GT 生物性能，从而使其
                                                               能够适用于组织工程和伤口敷料领域。

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