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第 36 卷第 5 期 精 细 化 工 Vol.36, No.5
201 9 年 5 月 FINE CHEMICALS May 2019

中药现代化技术
响应面法优化超高压提取黄精多糖工艺

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魏 炜，李彦伟，刘凤霞，许晓飞，刘志军
（大连理工大学 流体与粉体工程研究设计所，辽宁 大连 116024）

摘要：以黄精为研究对象，采用超高压提取技术，利用单因素实验及响应面方法优化了超高压提取黄精多糖的
工艺条件，以期获得较优的工艺参数，并将实验结果与传统工艺进行对比；对黄精多糖的抗氧化性进行了考察。
结果表明，超高压提取黄精多糖在压力 255 MPa、物料粒径 40 目、固液比 1∶17（固指物料质量，g；液指提取
剂体积，mL）、保压时间 9.5 min、提取温度为常温、提取剂为水的工艺条件下，多糖提取率为 25.01%；而传统
煎煮法在提取时间 90 min、提取温度 100 ℃的工艺条件下，多糖提取率为 19.83%，表明超高压提取黄精多糖方
式较为有效。当黄精多糖质量浓度为 1 g/L 时，其对 1,1-二苯基-2-三硝基苯肼（DPPH）自由基的清除能力达到
34.14%。
关键词：超高压；黄精多糖；提取率；工艺优化；响应面法；抗氧化性；中药现代化技术
中图分类号：R284.2 文献标识码：A 文章编号：1003-5214 (2019) 05-0875-07

Optimization of Ultrahigh Pressure Extraction of Polysaccharides from
Polygonatum cyrtonema Hua by Response Surface Methodology

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WEI Wei, LI Yan-wei, LIU Feng-xia, XU Xiao-fei, LIU Zhi-jun
（Fluid and Powder Engineering Research and Design Institute, Dalian University of Technology, Dalian 116024, Liaoning, China）

Abstract: The ultrahigh pressure extraction technology was used to extract polysaccharides from Polygonatum
cyrtonema Hua. The single factor experiment and response surface method were applied to obtain optimal
process parameters. Furthermore, the antioxidant activity of Polygonatum polysaccharides was investigated.
The results showed that the optimal extraction conditions were as follows: pressure 255 MPa, particle size
of raw material 40 mesh, ratio of raw material to extraction agent 1∶17 (solids refers the mass of the raw
material, g; liquid refers to the volume of the extractant agent, mL), extraction time 9.5 min, extraction
temperature room temperature，and extractant agent (water). Under these conditions, the extraction rate of
polysaccharides was 25.01%. While the traditional decocting method had an extraction rate of
polysaccharides of 19.83% at 100 ℃ for 90 min, which indicated that ultrahigh pressure extraction
technology was more effective technology for extracting Polygonatum polysaccharides. The scavenging
ability of polysaccharides (mass concentration 1 g/L) toward 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH)
free radical reached 34.14%.
Key words: ultrahigh pressure; Polygonatum polysaccharides; extraction rate; process optimization; response
surface method; antioxidant activities; modernization technology of traditional Chinese medicines

[1]
黄精（Polygonatum sibiricum）系百合科植物 ， 的，而且是取代食品中的抗氧化剂，保证食品安全
主要化学成分包括多糖、甾体皂苷、黄酮等 [2-3] 。现 健康的有效途径。
代药理证实黄精多糖具有降血压、降血糖、降血脂、 黄精多糖的萃取方式包括水煎煮法、热水浸提
抗炎、抗菌、抗癌、提高免疫力等作用 [4-10] 。将其 法、热回流法、超声波辅助法、微波辅助法以及超
添加于食品，不仅能够达到治疗某些慢性疾病的目 声波-微波协同等 [11-18] 。目前，对于超高压提取黄精

收稿日期：2018-10-13; 定用日期：2019-01-04; DOI: 10.13550/j.jxhg.20180756
作者简介：魏炜（1978—），女，副教授，博士，E-mail：hjweiwei@dlut.edu.cn。联系人：刘志军（1969—），男，教授，博士，E-mail：liuzj@dlut.edu.cn。