Page 153 - 精细化工2020年第2期

第 2 期 王 松，等: 标题多次顶空固相微萃取在大曲挥发性化合物定量中的应用 ·355·

通过考察不同样品量下化合物的 β 值和 R²，最终选 Food Chemistry, 2010, 58(4): 2462-2469.
[10] Chen Lu (陈璐), Wang Song (王松), Sun Jinyuan (孙金沅), et al.
择在 50 mg 样品量下对甲基庚烯酮和 5-甲基-2-噻吩
Principle and application of multiple headspace solid-phase
甲醛进行定量，在 35 mg 样品量下定量其余 44 种化 microextraction[J]. Fine Chemicals ( 精细化工 ), 2019, 36(10):
合物，其中己醛〔（2063.35±92.30） ng/g〕、愈创 1989-1994.
[11] Ezquerro Ó, Pons B, Tena M A T. Multiple headspace solid-phase
木酚〔（945.77±92.58） ng/g〕、2-甲基吡嗪〔（432.66± microextraction for the quantitative determination of volatile organic
5.26） ng/g〕、3-甲基-2(5H)-呋喃酮〔（3645.79± compounds in multilayer packagings[J]. Journal of Chromatography
A, 2003, 999(1): 155-164.
442.43） ng/g〕、γ-丁内酯〔（564.57±18.73 ）ng/g〕、
[12] Mónica F, Delia H. Optimization of multiple headspace solid-phase
β-石竹烯〔（104.88±0.60）ng/g〕在各类化合物中含 microextraction for the quantification of volatile compounds in dry
量最高。 fermented sausages[J]. Journal of Agricultural and Food Chemistry,
2007, 55(21): 8688.
采用 MHS-SPME 对化合物进行定量时，对实验 [13] Ezquerro Ó, Pons B, Tena M A T. Evaluation of multiple solid-phase
操作要求较高，数据分析工作量加大，当所需定量 microextraction as a technique to remove the matrix effect in
packaging analysis for determination of volatile organic compounds
化合物较多时，可能需要在不同样品量下分别对满
[J]. Journal of Chromatography A, 2003, 1020(8): 189-197.
足要求的化合物进行定量，因此，本实验中首先对 [14] Costa R, Tedone L, Grazia S D, et al. Multiple headspace-solid-phase
大曲中 6 大类共 46 种化合物进行定量，其中大部分 microextraction: An application to quantification of mushroom
volatiles[J]. Analytica Chimica Acta, 2013, 770(7): 1-6.
是文献报道具有一定生物活性的物质，而对酯类、 [15] Serrano E, Beltrán J, Hernández F. Application of multiple headspace-
酸类、醇类等化合物尚未分析，后续实验中仍需进 solid-phase microextraction followed by gas chromatography–mass
spectrometry to quantitative analysis of tomato aroma components[J].
一步探究该方法在这些化合物定量中的应用。
Journal of Chromatography A, 2009, 1216(1): 127-133.
MHS-SPME 继承了 HS-SPME 绿色、环保等优 [16] Zhang Xuena (张雪娜), Ye Changwen (叶长文), Zou Geng (邹更),
点，同时克服了固体样品的基质效应，可为大曲等 et al. Multiple headspace solid-phase microextraction after matrix
modification for the determination of ethyl carbamate in alcoholic
固体样品中挥发性化合物的定量提供良好的思路。 beverages using gas chromatography[J]. Chinese Journal of
Chromatography (色谱), 2011, 29(8): 701-705.
参考文献： [17] Meng Weiyi (孟维一), Huang Mingquan (黄明泉), Sun Baoguo (孙
宝国), et al. Identification of aroma-active compounds in Daqu by
[1] Li Hehe (李贺贺), Liu Jinlong (柳金龙), Liang Jinhui (梁金辉), et
al. Research on volatile compounds in 2 kinds of Gujinggong liquor[J]. HS-SPME-GC-MS/O[J]. Science and Technology of Food Industry
Journal of Food Science and Technology (食品科学技术学报), (食品工业科技), 2017, 38(6): 54-61.
2016, 34(1): 55-65. [18] Feng J, Sun M, Li L, et al. Multiwalled carbon nanotubes-doped
polymeric ionic liquids coating for multiple headspace solid-phase
[2] Li Anjun (李安军), Xu Xianghao (徐祥浩), Tang Youhong (汤有宏),
microextraction[J]. Talanta, 2014, 123(9): 18-24.
et al. Research on functional ingredients of Gujing Gongjiu by two
comprehensive dimensional gas chromatography-time of flight mass [19] Li Hehe (李贺贺), Hu Xiaomei (胡萧梅), Li Anjun (李安军), et al.
[J]. Liquor-making Science and Technology (酿酒科技), 2016, (1): Comparative analysis of volatile components in Gujinggong liquor
50-52. by headspace solid-phase microextraction and stir bar sorptive
[3] Liu Qingchuan (刘晴川), Chen Feng (陈峰), Liu Fei (刘飞), et al. extraction[J]. Food Science (食品科学), 2017, 38(4): 155-164.
Analyses of volatile compounds in Chinese liquor-gujing under [20] Zhou Qingwu (周庆伍), Li Anjun (李安军), Tang Youhong (汤有宏),
optimized headspace solid-phase microextraction (HS-SPME)[J]. et al. Research on volatile flavor components in Gujing Gongjiu liquor
Journal of Anhui Normal University (Natural Science) (安徽师范大 by two comprehensive dimensional gas chromatography- time of
学学报: 自然科学版), 2016, 39(6): 555-560, 574. flight mass[J].Liquor Making (酿酒), 2016, 43(2): 75-81.
[21] Xiao Nan (肖楠). Ethyl acetate inhibits experimental acute liver
[4] Yu qianwei (余乾伟). Traditional Baijiu brewing technology[M].
failure in mice and its mechanism of action[D]. Baoding: Hebei
beijing: China Light Industry Press (中国轻工业出版社), 2014.
University (河北大学), 2011.
[5] Lü Yunhuai (吕云怀), Wang Li (王莉), Wang Diqiang (汪地强), et
al. Analysis of the relations between flavoring substances of Daqu of [22] Huang Yanmei (黄艳梅), Lu Jianchun (卢建春), Li Anjun (李安军),
different flavor type and its styles and characteristics[J]. Liquor- et al. Analysis of flavor ing substances in Gujing Gongjiu liquor by
making Science and Technology (酿酒科技), 2012, (7): 72-75. GC/MS[J]. Liquor-making Science and Technology (酿酒科技),
2006, (7): 91-94.
[6] Fan W, Qian M C. Headspace solid phase microextraction and gas [23] Rincón A A, Pino V, Ayala J H, et al. Multiple headspace solid-phase
chromatography-olfactometry dilution analysis of young and aged microextraction for quantifying volatile free fatty acids in cheeses
Chinese “Yanghe Daqu” liquors[J]. Journal of Agricultural and Food [J]. Talanta, 2014, 129:183-190.
Chemistry, 2005, 53(20): 7931-7938.
[24] Guo Wenjie ( 郭文 杰 ), Lu Jianchun ( 卢建春 ). Research on
[7] Niu Jiao (牛姣), Fan Jianhui (樊建辉), Han Suna (韩素娜), et al.
characteristic flavouring components of Gujing’gong liquor[J]. Liquor-
Volatile flavoring compounds in Taoxiang medium- and high-
temperature Daqu[J]. Liquor-making Science and Technology (酿酒 making Science and Technology (酿酒科技), 2001, (5): 71-73.
科技), 2017, (11): 87-92. [25] Zhao D, Shi D, Sun J, et al. Characterization of key aroma
compounds in Gujinggong Chinese Baijiu by gas chromatography-
[8] Ming Hongmei (明红梅), Yao Xia (姚霞), Zhou Jian (周健), et al. olfactometry, quantitative measurements, and sensory evaluation[J].
Volatile flavoring compounds in medium/high temperature Nongxiang Food Research International, 2018, 105:616-627.
Daqu[J]. Liquor-making Science and Technology (酿酒科技), 2015, [26] South China Institute of Technology (华南工学院). Alcohol and
(6): 73-79. liquor technology[M]. Beijing: China Light Industry Press (中国轻
[9] Mo X, Xu Y, Fan W. Characterization of aroma compounds in 工业出版社), 1980.
Chinese rice wine Qu by solvent-assisted flavor evaporation and
headspace solid-phase microextraction[J]. Journal of Agricultural and （下转第 390 页）