Page 119 - 《精细化工》2021年第3期

第 38 卷第 3 期 精 细 化 工 Vol.38, No.3
202 1 年 3 月 FINE CHEMICALS Mar. 2021

功能材料
聚氨酯-Ch 水凝胶的制备及其作为伤口敷料的性能

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姚 周，范宏蕾，田 振，鲍俊杰，黄毅萍，许戈文
（安徽大学 化学化工学院，安徽省绿色高分子重点实验室，安徽 合肥 230601）

摘要：以 2,4-二羟基苯甲醛（DDBA）为扩链剂，丙烯酸羟乙酯（HEA）为封端剂制备了含有醛基的水性聚氨
酯（DPU），在温和的条件下引入不同含量的羧甲基壳聚糖（CMCh）溶液合成了 CMCh 席夫碱，接着其与丙烯
酰胺经自由基反应合成了聚氨酯-羧甲基壳聚糖席夫碱水凝胶（DPU-Ch）。通过 FTIR、SEM、力学性能、溶胀
保水性、抗菌性和血液相容性测试等对水凝胶结构和性能进行了表征。结果表明，水凝胶的机械性能随着 CMCh
含量的增加先增大后减小，水凝胶显示出良好的溶胀能力和保水能力；水凝胶对革兰氏阳性和阴性细菌菌株均
显示出良好的抗菌性能；水凝胶溶血率均低于 5%，表明其具有良好的细胞相容性，小鼠成纤维细胞（NIH3T3）
存活率在 90%以上证明其细胞毒性较低。当 CMCh 添加量为 DPU 树脂固含量的 4%时，DPU-Ch 水凝胶综合性
能最佳，溶胀比为 10.58，24 h 失水率为 31.1%，压缩强度为 0.70 MPa，溶血率为 2.4%，细胞存活率为 101%±1.7%，
抗菌性能良好。
关键词：醛基；羧甲基壳聚糖；抗菌性；聚氨酯水凝胶；席夫碱；功能材料
中图分类号：R318.08；TQ323.8 文献标识码：A 文章编号：1003-5214 (2021) 03-0539-07

Preparation of polyurethane-Ch hydrogel and its properties as wound dressing

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YAO Zhou, FAN Honglei, TIAN Zhen, BAO Junjie, HUANG Yiping, XU Gewen
（Key Laboratory of Environment-Friendly Polymer Materials of Anhui Province, School of Chemistry and Chemical
Engineering, Anhui University, Hefei 230601, Anhui, China）

Abstract: Waterborne polyurethane containing aldehyde group (DPU) was prepared using 2,4-dihydroxy-
benzaldehyde (DDBA) as chain extender and hydroxyethyl acrylate (HEA) as end-capper. Subsequently,
polyurethane-CMCh Schiff base was synthesized by introducing carboxymethylated chitosan (CMCh) with
different dosage under mild conditions. Then, a series of polyurethane-carboxymethyl chitosan Schiff base
(DPU-Ch) hydrogels were synthesized by free radical polymerization of polyurethane-CMCh Schiff base
and acrylamide. The structure and properties of hydrogels were characterized by FTIR, SEM, mechanical
properties, swelling water retention test, antibacterial test and blood compatibility test. The results showed
that the mechanical properties of hydrogels firstly increase and then decrease with the increase of CMCh
dosage. At the same time, the hydrogels exhibited good swelling ability and proper water retention capacity.
The prepared hydrogel had good antibacterial properties against E. coli and S. aureus. The hemolysis rates
of all hydrogels were lower than 5%, indicating that the hydrogels had good cell compatibility. Furthermore,
for these hydrogels, the survival rate of mouse embryo fibroblast NIH3T3 cells was above 90%, indicating
that their cytotoxic were lower. When the amount of CMCh added was 4% of the DPU resin solid content,
the DPU-Ch hydrogel had the best overall performance. The swelling ratio was 10.58, the 24 h water loss
rate was 31.1%, the compressive strength was 0.70 MPa, and the hemolysis rate was 2.4%. The survival
rate was 101%±1.7%, and the antibacterial performance was good.
Key words: aldehyde group; carboxymethyl chitosan; antibacterial properties; polyurethane hydrogel; Schiff
base; functional materials

收稿日期：2020-08-13; 定用日期：2020-11-26; DOI: 10.13550/j.jxhg.20200753
基金项目：安徽省自然科学基金项目（1808085QE173、1808085ME156）；安徽省高校科学研究项目（KJ2017A031、KJ2018ZD004）
作者简介：姚 周（1994—），男，硕士生，E-mail：1404720990@qq.com。联系人：许戈文（1961—），男，研究员，E-mail：gwxu@
ahu.edu.cn。