Page 169 - 《精细化工》2022年第7期
P. 169
第 7 期 杨佳慧,等: 包载槲皮素 P/O/W 多重 Pickering 乳液的制备及表征 ·1455·
此外体系组成中含有的内醇相(甘油以及一缩二丙 液制备的影响,得到 P/O/W 多重 Pickering 乳液最佳
二醇)以及不饱和脂肪酸(月见草油)等也可以增强 制备条件为:内醇相〔m(甘油)∶m(一缩二丙二醇)=
角质层脂质流动性,进而促进槲皮素的经皮输送 [32] 。 6∶4〕质量分数为 20%、疏水 SiO 2 质量分数为 3%、
总之,与槲皮素水分散液相比,P/O/W 多重乳液对 m(水相)∶m(P/O 初乳)=5∶5、亲水 SiO 2 质量分数为
槲皮素包载后可显著提高槲皮素的溶解度和透皮性 2%。
能,具有一定的皮肤靶向作用。 (2)采用 P/O/W 多重 Pickering 乳液对油水两
难溶性活性物槲皮素进行包载,有效提高了槲皮素
的溶解度及透皮性能。
(3)在之后的研究中可将该体系用于其他油水
两难溶性活性物的包载,考察该体系的普适性。
参考文献:
[1] BOOTS A W, HAENEN G R, BAST A. Health effects of quercetin:
From antioxidant to nutraceutical[J]. European Journal of Pharmacology,
2008, 585(2/3): 325-337.
[2] COSKUN O, KANTER M, KORKMAZ A, et al. Quercetin, a
flavonoid antioxidant, prevents and protects streptozotocin-induced
oxidative stress and β-cell damage in rat pancreas[J]. Pharmacological
Research, 2005, 51(2): 117-123.
[3] ROGERIO A P, KANASHIRO A, FONTANARI C, et al. Anti-
inflammatory activity of quercetin and isoquercitrin in experimental
murine allergic asthma[J]. Inflammation Research, 2007, 56(10):
402-408.
[4] COMALADA M, CAMUESCO D, SIERRA S, et al. In vivo
quercitrin anti-inflammatory effect involves release of quercetin,
which inhibits inflammation through down-regulation of the NF-κB
pathway[J]. European Journal of Immunology, 2005, 35(2): 584-592.
[5] TAKEKOSHI S, MATSUZAKI K, KITATANI K. Quercetin stimulates
melanogenesis in hair follicle melanocyte of the mouse[J]. Tokai
Journal of Experimental & Clinical Medicine, 2013, 38(4): 129-134.
[6] KITAGAWA S, TANAKA Y, TANAKA M, et al. Enhanced skin
delivery of quercetin by microemulsion[J]. Journal of Pharmacy &
Pharmacology, 2010, 61(7): 855-860.
[7] KHALID N, KOBAYASHI I, NEVES M A, et al. Microchannel
emulsification study on formulation and stability characterization of
monodisperse oil-in-water emulsions encapsulating quercetin[J].
Food Chemistry, 2016, 212: 27-34.
图 9 槲皮素水分散液和槲皮素多重乳液透皮 12 h 后在 [8] LIU L, TANG Y H, GAO C, et al. Characterization and biodistribution
角质层、活力表皮+真皮层中的槲皮素含量(A); in vivo of quercetin-loaded cationic nanostructured lipid carriers[J].
Colloids and Surfaces B: Biointerfaces, 2014, 115: 125-131.
透皮处理后的猪皮截面显微镜图(B:a—槲皮素水
[9] CHEN X, MCCLEMENTS D J, WANG J, et al. Coencapsulation of
分散液处理猪皮荧光通道;b—槲皮素水分散液处 (–)-epigallocatechin-3-gallate and quercetin in particle-stabilized
理猪皮自然光通道;c—槲皮素多重乳液处理猪皮 W/O/W emulsion gels: Controlled release and bioaccessibility[J].
Journal of Agricultural and Food Chemistry, 2018, 66(14): 3691-3699.
荧光通道;d—槲皮素多重乳液处理猪皮自然光通道)
Fig. 9 Quercetin content in stratum corneum, active [10] CARLA C, OCTAVIO D, RAMON P, et al. Cross-linked chitosan/
liposome hybrid system for the intestinal delivery of quercetin[J].
epidermi+dermis after treatment with quercetin Journal of Colloid and Interface Science, 2016, 461: 69-78.
aqueous dispersion and quercetin-loaded double [11] ADITYA N P, ADITYA S, YANG H, et al. Co-delivery of hydrophobic
emulsion (A); Microscope images of cross-section curcumin and hydrophilic catechin by a water-in-oil-in-water double
of pig skin after transdermal treatment (B: a— emulsion[J]. Food Chemistry, 2015, 173: 7-13.
fluorescence channel of pig skin treated with [12] GALLARATE M, CARLOTTI M E, TROTTA M, et al. On the
quercetin aqueous dispersion; b—natural light stability of ascorbic acid in emulsified systems for topical and cosmetic
channel of pig skin treated with quercetin aqueous use[J]. International Journal of Pharmaceutics, 1999, 188(2): 233-241.
dispersion; c—fluorescence channel of pig skin [13] BINKS B P. Particles as surfactants—Similarities and differences[J].
treated with quercetin-loaded double emulsion; Current Opinion in Colloid & Interface Science, 2002, 7(1/2): 21-41.
d—natural light channel of skin treated with [14] LAMBA H, SATHISH K, SABIKHI L. Double emulsions: Emerging
quercetin-loaded double emulsion) delivery system for plant bioactives[J]. Food and Bioprocess
Technology, 2015, 8(4): 709-728.
3 结论 [15] WANG J, SUN Y J, YU M Y, et al. Emulsions stabilized by highly
hydrophilic TiO 2 nanoparticles via Van der Waals attraction[J].
Journal of Colloid and Interface Science, 2021, 589: 378-387.
(1)考察了各因素对 P/O/W 多重 Pickering 乳 [16] SCHUSTER S, BERNEWITZ R, GUTHAUSEN G, et al. Analysis of