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第 35 卷第 11 期                            精   细   化   工                                 Vol.35, No.11
             2018 年 11 月                             FINE CHEMICALS                                Nov.   2018


              功能材料
                                    分子印迹聚合物纯化甘草酸



                          郗万宝      1,2 ,郭瑞丽      1,2* ,张建树      1,2 ,徐邵合       1,2 ,裴文静      1,2

                 (1.  石河子大学  化学化工学院,新疆  石河子  832003;2.  新疆兵团化工绿色过程重点实验室,新疆  石
                 河子  832003)

                 摘要:以甘草酸、不同单体(甲基丙烯酸、甲基丙烯酸羟乙酯和 4-乙烯基吡啶)和交联剂(二乙烯基苯和乙二
                 醇二甲基丙烯酸酯)为原料,在乙腈和 N,N-二甲基甲酰胺(DMF)的混合液中,以偶氮二异丁腈为引发剂,采
                 用沉淀聚合法制备了一系列分子印迹聚合物(MIPs)。采用 FTIR、SEM 和 BET 对 MIPs 进行了表征与测试,优
                 化了 MIPs 的制备工艺。结果表明:当交联剂为二乙烯基苯、单体为甲基丙烯酸、甘草酸(0.16 mmol)与甲基丙烯
                 酸的物质的量比为 1 : 10、致孔剂为 25 mL 乙腈和 8 mL DMF 的混合液时,MIPs 对甘草酸的吸附性能最好。在
                 5 g/L 的甘草酸溶液中,MIPs 对甘草酸的平衡吸附量可达 398.5 mg/g,印迹因子为 3.73。并对 MIPs 的吸附动力
                 学和吸附等温线进行了考察。利用分子印迹固相萃取技术(MISPE)对甘草提取物进行精制,将甘草提取物中
                 甘草酸的质量分数由 11.27%提高到 73.10%,回收率为 86.87%。
                 关键词:甘草酸;分子印迹聚合物;沉淀聚合;氢键;功能材料
                 中图分类号:O69; O658; O657      文献标识码:A     文章编号:1003-5214 (2018) 11-1859-08


                   Purification of Glycyrrhizic Acid by Molecularly Imprinted Polymers


                                  1,2
                                                                   1,2
                                                                                 1,2
                                                                                                1,2
                        XI Wan-bao , GUO Rui-li 1,2* , ZHANG Jian-shu , XU Shao-he , PEI Wen-jing
                 (1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, Xinjiang, China; 2. Key Laboratory
                 for Green Process of Chemical Engineering of Xinjiang Bingtuan, Shihezi 832003, Xinjiang, China)
                 Abstract: A series  of  molecularly imprinted polymers (MIPs) were prepared by  precipitation
                 polymerization in acetonitrile and N,N-dimethylformamide (DMF) mixed solvents using glycyrrhizic acid,
                 different monomers (methacrylic acid, hydroxyethyl methacrylate and 4-vinyl pyridine) and crosslinking
                 agents (divinyl benzene and ethylene glycol dimethacrylate) as raw materials and azodiisobutyronitrile as
                 initiator. The MIPs were characterized by FTIR, SEM and BET. Moreover, the preparation process of MIPs
                 was optimized. The results showed that when divinylbenzene was used as crosslinking agent, the molar
                 ratio of glycyrrhizic acid(0.16 mmol)  to methacrylic acid was 1 : 10, and a mixture of acetonitrile (25 mL)
                 and DMF (8 mL) was adopted as porogen, the obtained MIPs showed the best adsorption to glycyrrhizic
                 acid. In 5 g/L glycyrrhizic acid solution, the equilibrium adsorption capacity of MIPs to glycyrrhizic acid
                 was 398.5 mg/g and the imprinting factor was 3.73. The adsorption kinetics and adsorption isotherm of
                 MIPs were studied. Meanwhile, the licorice extract  was refined  by molecularly imprinted solid  phase
                 extraction  (MISPE), and the mass fraction of  glycyrrhizic acid increased  from 11.27% to 73.10%  with
                 86.87% recovery yield.
                 Key words: glycyrrhizic acid; molecularly imprinted polymers; precipitation polymerization; hydrogen bonds;
                 functional materials
                 Foundation item: National Natural Science Youth Foundation of China (21006064)


                                                                                            [3]
                                                                                                       [4]
                 甘草酸是甘草根中的一种主要活性物质,具有                          草酸的分离纯化方法有超声萃取 、微波萃取 、超
                                                                                     [6]
                                                                                                 [7]
                                                                       [5]
            抗炎、解毒、抗氧化、保肝、抗病毒等功效                     [1-2] 。甘   热水萃取 、超临界萃取 、胶束萃取 、离子液体

                 收稿日期:2018-01-15;  定用日期:2018-05-05; DOI: 10.13550/j.jxhg.20180045
                 基金项目:国家自然科学基金青年基金项目(21006064)
                 作者简介:郗万宝(1987—),男,硕士生。联系人:郭瑞丽(1978—),女,教授,电话:0993-2057277,E-mail:grli@shzu.edu.cn。
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