Page 98 - 201809

第 35 卷第 9 期 精 细 化 工 Vol.35, No.9
201 8 年 9 月 FINE CHEMICALS Sept. 2018

生物工程
G31 产细菌纤维素结构表征及其面膜性能测试

1
1*
1
1
唐 静 ，惠 明 ，廖爱美 ，田 青 ，黄继红 1,2
（1. 河南工业大学 生物工程学院，河南 郑州 450001；2. 郑州中食农产品加工研究院，河南 郑州
450001）
摘要：以葡糖醋杆菌 G31 为菌种，用 HS 培养基发酵制备细菌纤维素（BC），产物湿重产率为 320.1 g/L。利用
13
蒽酮比色法、酶水解法初步证明发酵产物为纤维素，采用 FTIR、SEM、XRD、CP/MAS CNMR、DSC 和 TG
对其结构进行了表征并测试了其性能。结果表明：发酵产物具有超细三维网状结构，属于结晶度为 92.42%的纤
维素Ⅰ型，具有良好的持水性和拉伸性。分子质量测定结果显示：M w =5737，属于低相对分子质量 BC，其氧气
2
2
3
透过率和水蒸气透过率分别为 913.553.23 cm /(m d0.1 MPa)和 656.153.92 g/(m d)，表明该 BC 具有更好的锁
水能力，为其在面膜领域的应用奠定了基础。
关键词：细菌纤维素；发酵；保湿；面膜应用；生物工程
中图分类号：TQ352.2 文献标识码：A 文章编号：1003-5214 (2018) 09-1524-05

Structure Characterization and Performance Test of Bacterial Cellulose
Produced by Gluconacetobacter Xylinum G31

1*
1
1
1,2
1
TANG Jing , HUI Ming , LIAO Ai-mei , TIAN Qing , HUANG Ji-hong
（ 1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China;
2. Zhengzhou Zhongshi Research Institute of Agriculture Products Processing, Zhengzhou 450001, Henan, China）
Abstract: Bacterial cellulose (BC) with a wet weight of 320.1 g/L was fermented by Gluconacetobacter
xylinum G31 strain in Hestrin-Schramm (HS) medium. Anthrone colorimetry and enzymatic hydrolysis was
used to characterize the fermentation product and the results proved that the product was cellulose. The
structure and performances of fermentation product were characterized by FTIR, SEM, XRD, CP/MAS
13 CNMR, DSC and TG techniques. The results showed that the fermentation product had an ultrafine
three-dimensional network structure and belonged to cellulose type I with a crystallinity of 92.42%.
Moreover, it exhibited good water-holding ability and stretchability. Its molecular weight was measured
(M w=5737), which belonged to low molecular weight BC. The oxygen transmission rate and water vapor
2
2
3
transmission rate of BC facial mask were 913.553.23 cm /(m d0.1 MPa) and 656.153.92 g/(m d),
respectively, indicating that BC facial mask had better water-holding capacity, which laid a foundation for
its application in the field of facial mask.
Key words: bacterial cellulose; ferment; water-holding; facial mask application; biological engineering
Foundation items: Henan University of Technology “Excellent Doctoral, Master Degree Thesis
Cultivation” Project (2017); Open Project of Henan Provincial Key Laboratory of Transformation and
Utilization of Cereals (PL2016006)

[1]
细菌纤维素（Bacterial cellulose，简称 BC）是 接而成的直链多糖 。与植物纤维素相比，细菌纤
由醋杆菌属、沙门氏菌属等微生物产生的胞外产物， 维素具有纳米级的超精细网状结构，较高的化学纯
其结构可视为由吡喃葡萄糖单体以-1,4-糖苷键连 度、结晶度，以及良好的持水保湿能力和生物相容

收稿日期：2018-03-22; 定用日期：2018-06-11; DOI: 10.13550/j.jxhg.20180198
基金项目：河南工业大学“优秀博士、硕士学位论文培育”项目（2017）；河南省谷物资源转化与利用重点实验室开放课题（PL2016006）
作者简介： 唐 静（1992—），女，硕士生。联系人：惠 明（1969—），男，博士，教授，E-mail:q2453857427@163.com。