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第 36 卷第 1 期 精 细 化 工 Vol.36, No.1
201 9 年 1 月 FINE CHEMICALS Jan. 2019

功能材料
用于稳定 Pickering 乳液的氨基化

明胶纳米颗粒的制备

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薛万波 ，王春华 ，谭 欢 ，林 炜
（1. 四川大学 皮革化学与工程教育部重点实验室，四川 成都 610065；2. 成都大学 四川抗菌素工业研
究所，药食同源植物资源开发四川省高校重点实验室，四川 成都 610065）
摘要：以氨基化改性的 B 型明胶为原料，通过二次去溶剂法可制得具有良好单分散性（PDI≈0.068）的氨基化
明胶纳米颗粒（Aminated gelatin nanoparticles，AGNPs），其粒径约为 240 nm，表面带正电荷（ζ≈35 mV），并
考察了制备过程中溶液 pH 对 AGNPs 粒径以及分散性的影响。结果表明：当 pH≈4.0 时，制备的 AGNPs 粒径
较小（≈240 nm），分散较为均匀。SEM、AFM 及 TEM 测试结果表明：AGNPs 为一种粒径窄分布的球形纳米
颗粒；相比明胶纳米颗粒（Gelatin nanoparticles，GNPs），AGNPs 刚性结构特点增强，且质地较为均匀。颗粒
表面润湿性测试结果显示，AGNPs 的三相接触角（θ ow = 67± 5）比 GNPs（θ ow = 31± 6）显著增大，表明 AGNPs
的表面疏水性有所增强。以 AGNPs 为稳定剂制备的水包油（O/W）型 Pickering 乳液 4 ℃下储存 9 个月未出现
分层，表现出较高的稳定性。
关键词：氨基化明胶；氨基化明胶纳米颗粒；刚性结构；三相接触角；Pickering 乳液；功能材料
中图分类号：TB383.1 文献标识码：A 文章编号：1003-5214 (2019) 01-0031-07

Preparation of Aminated Gelatin Nanoparticles Used to
Stabilize Pickering Emulsion

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XUE Wan-bo , WANG Chun-hua , TAN Huan , LIN Wei
（1. Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu
610065, Sichuan, China; 2. Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan
Education Department, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610065, Sichuan,
China）
Abstract: Fairly monodispersed aminated gelatin nanoparticles (AGNPs, PDI≈0.068) with an average size
of 240 nm were prepared by a two-step desolvation method using amino-modified gelatin of type B as raw
materials. The particles were positively charged (≈35 mV). The effects of pH value of solution on the
particle size and dispersity of AGNPs were investigated in the preparation process. The results showed that
the prepared AGNPs exhibited uniform particle size distribution with a smaller particle size of
approximately 240 nm at pH≈4.AFM,SEM and TEM analysis revealed that the AGNPs exhibited spherical
morphology with a narrow particle size distribution. Compared with gelatin nanoparticles (GNPs), AGNPs
had stronger rigid structure and more uniform texture. Particle surface wettability test results demonstrated
that the three-phase contact angle of AGNPs (θ ow=67±5) was higher than that of GNPs (θ ow=31±6),
indicating that the surface hydrophobicity of AGNPs increased. The Pickering emulsion stabilized by
AGNPs could be stored for 9 months at 4 ℃ without stratification and showed good stability.
Key words: aminated gelatin; AGNPs; rigid structure; three-phase contact angle; Pickering emulsion;
functional materials
Foundation items: National Natural Science Foundation of China (21476148); Innovation Team Program of
Science & Technology Department of Sichuan Province (Grant 2017TD0010)

收稿日期：2018-03-30; 定用日期：2018-07-03; DOI: 10.13550/j.jxhg.20180235
基金项目：国家自然科学基金资助项目（21476148）；四川省青年科技创新研究团队（Grant 2017TD0010）
作者简介：薛万波（1990—），男，硕士生。联系人：林 炜（1972—），女，教授，电话：028-85460819，E-mail：wlin@scu.edu.cn。