Page 101 - 精细化工2019年第12期

第 12 期 黄秋容，等: 肉桂内生菌 Sphingomonas sp. Z45 生物转化肉桂醇生成天然 2-苯乙醇 ·2429·

平), et al. Studies on the production of 2-phenylethanol from
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of Bioengineering (生物工程学报), 2016, 32(9): 1151-1163.
[6] Niu Mingfu (牛明福), Li Yaheng (李亚恒), Chen Jinshuai (陈金帅),
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Cruvier Maxima and analysis of its high temperature tolerance[J].

图 7 底物加入量对转化反应的影响 Food and Fermentation Industries (食品与发酵工业), 2018, 44(2):
Fig. 7 Effect of substrate addition on the conversion reaction 15-20.
[7] Huang Xiaoping (黄筱萍), Liu Lan (刘兰), Xiong Dawei (熊大维),
et al. Continuous transformation of 2-phenylethanol by yeast resting
2.4 验证实验 cells coupled with in situ separation technique[J]. Food and
在单因素实验所得的最优条件下进行重复验证 Fermentation Industries (食品与发酵工业), 2018, 44(10): 20-24.
[8] Xing Chenguang (邢晨光). Screening of 2-phenylethanol marine
实验，平行 3 次实验，结果见表 1。
yeast and optimization of fermentation conditions[J]. Biochemistry
(生物化工), 2017, 3(2): 16-19.
表 1 验证实验结果 [9] Chreptowicz K, Mierzejewska J. Enhanced bioproduction of
Table 1 Validation of test results 2-phenylethanol in a biphasic system with rapeseed oil[J]. New
Biotechnology, 2018, 42: 56-61.
实验号 肉桂醇转化率/% 2-PE 质量浓度/(g/L)
[10] Wang H, Dong Q, Guan A, et al. Synergistic inhibition effect of
1 58.98 1.47 2-phenylethanol and ethanol on bioproduction of natural
2 60.12 1.50 2-phenylethanol by Saccharomyces cerevisiae and process
3 58.37 1.46 enhancement[J]. Journal of Bioscience and Bioengineering, 2011,
112(1): 26-31.
平均值 59.16 1.48
[11] Etschmann M M W, Sell D, Schrader J. Screening of yeasts for the
RSD/% 1.50 1.41 production of the aroma compound 2-phenylethanol in a molasses
based medium[J]. Biotechnology Letters, 2003, 25(7): 531-536.
实验结果表明，优化的工艺条件稳定可行。 [12] Eshkol N, Sendovski M, Bahalul M, et al. Production of
2-phenylethanol from L-phenylalanine by a stress tolerant
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[13] Tang Yuqi (唐育岐), Liu Tianming (刘天明), Zhao Xiubao (赵修报),
本文以肉桂醇为原料，利用筛选到的肉桂内生 et al. Screening of yeast strains producing 2-phenylethyl alcohol and
菌 Sphingomonas sp. Z45 生产 2-苯乙醇。利用 GC-MS optimization of culture medium[J]. Chinese Food Additives (中国食
品添加剂), 2012, (3): 137-142.
检测到代谢中间体——苯乙醛，推测该反应代谢途
[14] Cui Zhifeng (崔志峰), Che Zhibo (车智博), Yang Xiao (杨霄), et al.
径为：肉桂醇先氧化、脱羧生成苯乙醛，苯乙醛再 Breeding of 2-phenylethanol-tolerant high-yielding yeast strain[J].
加氢还原成 2-苯乙醇。在初始 pH = 7、装有 20 mL Journal of Zhejiang University of Technology (浙江工业大学学报),
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发酵培养基的 150 mL 三角瓶中接种体积分数为 5%
[15] Mei Jianfeng ( 梅建凤 ), Min Hang ( 闵航 ). Breeding of
菌体，培养 24 h 后加入底物肉桂醇 2.5 g/L，于 30 2-phenylethanol by biotransformation method[J]. Food and
℃、转速为 200 r/min 的摇床反应 12 h 后，肉桂醇 Fermentation Industry (食品与发酵工业), 2007, (5): 22-24.
[16] Nicholas R L, James J, Aziz E, et al. Data mining for discovery of
的转化率达到 59.16%，转化体系中 2-苯乙醇的质量
endophytic and epiphytic fungal diversity in short-read genomic data
浓度为 1.48 g/L。实验结果表明该工艺稳定可行。本 from deciduous trees[J]. Fungal Ecology, 2018, 35: 1-9.
研究采用原材料成本相对较低的肉桂醇生产 2-苯乙 [17] Zhu Lifang (朱丽芳), Ma Li (马丽), Liu Xiongmin (刘雄民), et al.
Microbial Mucor sp. analysis of degradation pathway of
醇，为微生物转化生产 2-苯乙醇探索了一条新途径。
acetophenone from cinnamic acid[J]. Fine Chemical (精细化工),
2013, 30(4): 439-441, 446.
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