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·2424·                            精细化工   FINE CHEMICALS                                  第 36 卷

            酶错误识别引诱分子,从而启动过氧化氢分流反应                                 structure required for α-hydroxylation[J]. Lipids, 2000, 35(4): 365-371.
            得到化合物Ⅰ,进而由化合物Ⅰ催化底物进行单加                             [7]   Matsunaga I, Ueda A, Sumimoto T, et al. Site-directed mutagenesis
                                                                   of  the  putative  distal  helix  of  peroxygenase  cytochrome  P450s[J].
            氧反应。引诱分子的引入克服了底物辅助激活机                                  Archives of Biochemistry & Biophysics, 2001, 394(1): 45-53.
            制 [13-14] 。因此,下步工作准备进行引诱分子筛选,                      [8]   Girhard  M,  Schuster  S,  Dietrich  M,  et al.  Cytochrome  P450s
            使玫瑰产色链霉菌 P450s 酶催化泼尼松龙的转化率                             monooxygenase from Clostridium acetobutylicum: a new alpha-fatty
                                                                   acid hydroxylase[J]. Biochem Biophys Res Commun, 2007, 362(1):
            提高。另外,由于酶受过氧化氢毒性影响,活性受                                 114-119.
            到部分抑制,因此对过氧化氢的浓度控制将是一个                             [9]   Bui  P  H,  Hankinson  O.  Functional  characterization  of  human
            研究方向,比如:光驱动原位生成过氧化氢法                      [15] ,       cytochrome  P450s 2S1  using  a  synthetic  gene-expressed protein  in
                                                                   Escherichia coli[J].  Molecular  Pharmacology,  2009,  76(5):  1031-
            不断释放出反应所需过氧化氢。                                         1043.
                                                               [10]  Niraula  N  P,  Kanth  B  K,  Sohng  J  K,  et al.  Hydrogen  peroxide-
            参考文献:                                                  mediated  dealkylation  of  7-ethoxycoumarin  by  cytochrome  P450s
            [1]   Trower  M  K,  Sariaslani  F  S,  Kitson  F  G.  Xenobiotic  oxidation  by   (CYP107AJ1) from Streptomyces peucetius ATCC27952[J]. Enzyme
                 cytochrome  P-450-enriched  extracts  of  Streptomyces griseus[J].   & Microbial Technology, 2011, 48(2): 181-186.
                 Biochemical  &  Biophysical  Research  Communications,  1988,   [11]  Berrie J R, Williams R A, Smith K E. Microbial transformations of
                 157(3): 1417-1422.                                steroids-Ⅻ.  Progesterone  hydroxylation  profiles  are  modulated  by
            [2]   Xue  Y,  Wilson  D,  Zhao  L,  et al.  Hydroxylation  of  macrolactones   post-translational  modification  of  an  electron  transfer  protein in
                 YC-17 and narbomycin is mediated by the pikC-encoded cytochrome   Streptomyces roseochromogenes[J]. Journal of Steroid Biochemistry
                 P450s  in  Streptomyces venezuelae[J].  Chemistry  &  Biology,  1998,   & Molecular Biology, 2001, 77(1): 87-96.
                 5(11): 661-667.                               [12]  Fujii Y, Kabumoto H, Nishimura K, et al. Purification, characterization,
            [3]   Dangi B, Kim K H, Kang S H, et al. Tracking down a novel steroid   and  directed  evolution  study  of  a  vitamin  D 3  hydroxylase  from
                 hydroxylating  promiscuous  cytochrome  P450,  CYP154C8  from   Pseudonocardiaautotrophica[J]. Biochemical & Biophysical Research
                 streptomyces sp W2233-SM[J]. Chembiochem A European Journal of   Communications, 2009, 385(2): 170-175.
                 Chemical Biology, 2018,19(10): 1066-1077.     [13]  Shoji  O,  Kunimatsu  T,  Kawakami  N,  et al.  Highly  selective
            [4]   Beckman M J, Deluca H F. Regulation of renal vitamin D receptor is   hydroxylation  of  benzene  to  phenol  by  wild-type  cytochrome
                 an  important  determinant  of  1α,  25-dihydroxyvitamin  D 3  levels  in   P450BM3  assisted  by  decoy  molecules[J].  Angewandte  Chemie
                 vivo[J]. Archives of Biochemistry & Biophysics, 2002, 401(1): 44-52.   International Edition, 2013, 52(26): 6606-6610.
            [5]   Shoji O, Watanabe Y. Peroxygenase reactions catalyzed by cytochromes   [14]  Ma  N,  Chen  Z,  Chen  J,  et al.  The  use  of  dual-functional  small
                 P450s[J]. Journal of Biological Inorganic Chemistry, 2014, 19(4/5):   molecules  in  generating  an  efficient  cytochrome  P450BM3
                 529-539.                                          peroxygenase[J]. Angewandte Chemie, 2018, 57(26): 7628-7633.
            [6]   Isamu Matsunaga, Tatsuo Sumimoto, Atsuo Ueda, et al. Fatty acid-   [15]  Girhard  M,  Kunigk  E,  Tihovsky  S,  et al.  Light-driven  biocatalysis
                 specific, regiospecific, and stereospecific hydroxylation by cytochrome   with cytochrome P450 peroxygenases[J]. Biotechnology & Applied
                 P450s (CYP152B1) from Sphingomonaspaucimobilis: Substrate     Biochemistry, 2013, 60(1): 111-118.



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