Page 141 - 《精细化工》2020年第12期

第 12 期 张金丽，等: Ag NPs 改性鱼鳞明胶-琼脂复合膜的制备及性能表征 ·2503·

实验评价不同银含量复合膜对 Escherichia coli 等 8 and food safety: Barrier materials, antimicrobials and sensors[J].
Journal of Colloid and Interface Science, 2011, 363(1): 1-24.
种致病菌的抑菌性能，结果发现，当复合膜中银含 [4] BAHRAMI A, MOKARRAM R R, KHIABANI M S, et al.
量为明胶干重的 0.2%时，制备的复合膜对 Escherichia Physico-mechanical and antimicrobial properties of tragacanth/
hydroxypropyl methylcellulose/beeswax edible films reinforced with
coli 等菌无任何抑菌效果。而本研究利用鱼鳞明胶 silver nanoparticles[J]. International Journal of Biological
还原制备的 Ag NPs 作为填充物制备的鱼鳞明胶-琼 Macromolecules, 2019, 129: 1103-1112.
[5] KANMANI P, RHIM J W. Physicochemical properties of
脂-Ag NPs 复合膜在银含量更低（0.04%~0.19%）下
gelatin/silver nanoparticle antimicrobial composite films[J]. Food
即具有明显的抗菌性能。 Chemistry, 2014, 148: 162-169.
[6] SHANKAR S, WANG L F, RHIM J W. Preparations and
characterization of alginate/silver composite films: Effect of types of
表 3 Ag NPs 改性鱼鳞明胶-琼脂复合膜对大肠杆菌、枯 silver particles[J]. Carbohyd Polym, 2016, 146: 208-216.
草芽孢杆菌、粪肠球菌和霍乱弧菌的抑菌效果 [7] RHIM J W, WANG L F, HONG S I. Preparation and characterization
Table 3 Antimicrobial activities of Ag NPs modified fish of agar/silver nanoparticles composite films with antimicrobial
scale gelatin-agar composite films against E. Coli, activity[J]. Food Hydrocolloids, 2013, 33(2): 327-335.
Bacillus subtilis, Enterococcus faecalis and [8] BUMBUDSANPHAROKE N, KO S. Nano-food packaging: An
Vibrio cholerae overview of market, migration research, and safety regulations[J]. J
Food Sci, 2015, 80(5): R910-R923.
抑菌圈直径/mm [9] RHIM J W, WANG L F, LEE Y H, et al. Preparation and
银含量/% 枯草芽孢 characterization of bionanocomposite films of agar and silver
大肠杆菌 粪肠球菌 霍乱弧菌 nanoparticles laser ablation method[J]. Carbohyd Polym, 2014, 103:
杆菌
456-465.
0 0 0 0 0 [10] KANMANI P, RHIM J W. Physical, mechanical and antimicrobial
properties of gelatin based active nanocomposite films containing
0.04 8.69±0.13 6.94±0.21 6.53±0.46 9.14±0.25
agnps and nanoclay[J]. Food Hydrocolloids, 2014, 35: 644-652.
0.08 9.45±0.62 8.29±0.41 7.87±0.69 10.47±0.11 [11] HOSSEINI S F, GÓMEZ-GUILLÉN M C. A state-of-the-art review
0.12 11.08±0.70 9.49±0.35 8.76±0.56 11.84±0.24 on the elaboration of fish gelatin as bioactive packaging: Special
emphasis on nanotechnology-based approaches[J]. Trends Food Sci
0.15 11.70±0.80 10.22±0.39 9.28±0.49 13.35±0.12
Tech, 2018, 79: 125-135.

0.19 12.74±0.56 11.58±0.63 9.51±0.39 14.18±0.55 [12] MOHAJER S, REZAEI M, HOSSEINI S F. Physico-chemical and
microstructural properties of fish gelatin/agar bio-based blend
films[J]. Carbohyd Polym, 2017, 157: 784-793.
3 结论 [13] SINHA T, AHMARUZZAMAN M, SIL A K, et al. Biomimetic
synthesis of silver nanoparticles using the fish scales of labeo rohita
利用鱼鳞明胶还原银氨离子成功制得了 Ag and their application as catalysts for the reduction of aromatic nitro
compounds[J]. Spectrochimica Acta Part A, Molecular and
NPs，Ag NPs 以球形形貌嵌在鱼鳞明胶肽链上，其 Biomolecular Spectroscopy, 2014, 131: 413-423.
平均粒径为(9.3±1.8) nm，粒径较为均一。将 Ag NPs [14] LIU Y, LIU X H, WANG X. Biomimetic synthesis of gelatin
polypeptide-assisted noble-metal nanoparticles and their interaction
与鱼鳞明胶-琼脂溶液混合，制备了银含量为 0.04%~ study[J]. Nanoscale Res Lett, 2011, 6(1): 22.
0.19%的鱼鳞明胶-琼脂-Ag NPs 复合膜，Ag NPs 与 [15] FU M X, LI Q B, SUN D H, et al. Rapid preparation process of silver
nanoparticles by bioreduction and their characterizations[J]. Chinese
鱼鳞明胶-琼脂复合膜具有优异的相容性，并存在强 J Chem Eng, 2006, 14(1): 114-117.
的化学相互作用。随着银含量的增加，复合膜的色 [16] WENG W Y (翁武银), LIU G M (刘光明), SU W J (苏文金), et al.
Preparation and thermal stability of gelatin edible films from shark
泽逐渐变深，透明度下降，复合膜的断裂伸长率、 skins[J]. Journal of Fisheries of Ching (水产学报), 2011, 35(12):
水蒸气阻隔性能和耐水性能显著增强，而复合膜的 1890-1896.
[17] BASUMATARY K, DAIMARY P, DAS S K, et al. Lagerstroemia
厚度和拉伸强度无明显变化。添加 Ag NPs 后，复合 speciosa fruit-mediated synthesis of silver nanoparticles and its
膜对大肠杆菌、枯草芽孢杆菌、粪肠球菌和霍乱弧 application as filler in agar based nanocomposite films for
antimicrobial food packaging[J]. Food Packaging and Shelf Life,
菌均显示强的抑菌性能。因此，以鱼鳞明胶生物还 2018, 17: 99-106.
原法制备的 Ag NPs 不仅可以在较低银含量下有效 [18] XU Y Q (徐叶琼), WENG W Y (翁武银). Effect of thermal
treatment temperature on properties of gelatin-konjac flour composite
改善鱼鳞明胶-琼脂复合膜的理化性能，还具有良好 films[J]. Food Science (食品科学), 2019, 40(3): 202-207.
的抗菌性能，将鱼鳞明胶还原制备的 Ag NPs 与鱼鳞 [19] ORSUWAN A, SHANKAR S, WANG L F, et al. Preparation of
antimicrobial agar/banana powder blend films reinforced with silver
明胶-琼脂混合是制备低银含量明胶抗菌包装膜的 nanoparticles[J]. Food Hydrocolloids, 2016, 60: 476-485.
有效方法。 [20] DENAVI G A, PÉREZ-MATEOS M, AŇÓN M C, et al. Structural
and functional properties of soy protein isolate and cod gelatin blend
参考文献： films[J]. Food Hydrocolloids, 2009, 23(8): 2094-2101.
[21] GIMÉNEZ B, LÓPEZ DE LACEY A, PÉREZ-SANTÍN E, et al.
[1] RHIM J W, PARK H M, HA C S. Bio-nanocomposites for food Release of active compounds from agar and agar-gelatin films with
packaging applications[J]. Prog Polym Sci, 2013, 38(10): 1629-1652. green tea extract[J]. Food Hydrocolloids, 2013, 30(1): 264-271.
[2] OTHMAN S H. Bio-nanocomposite materials for food packaging [22] CHAMBI H, GROSSO C. Edible films produced with gelatin and
applications: Types of biopolymer and nano-sized filler[J]. casein cross-linked with transglutaminase[J]. Food Research
Agriculture and Agricultural Science Procedia, 2014, 2: 296-303. International, 2006, 39(4): 458-466.
[3] DUNCAN T V. Applications of nanotechnology in food packaging （下转第 2509 页）