Page 158 - 《精细化工》2020年第8期

·1656· 精细化工 FINE CHEMICALS 第 37 卷

[4] FALDINI C, TRAINA F, PERNA F, et al. Surgical treatment of aseptic
forearm nonunion with plate and opposite bone graftstrut. Autograft
or allograft?[J]. International Orthopaedics, 2015, 39(7): 1343-1349.
[5] LANGER R, VACANTI J P. Tissue engineering[J]. Science, 1993,
260(5110): 920-926.
[6] HE C L (贺超良), TANG Z H (汤朝晖), TIAN H Y (田华雨), et a1.
Progress in the development of biomedical polymer materials fabricated
by 3-dimensional printing technology[J]. Acta Polymerica Sinica (高
分子学报), 2013, 44(6): 722-732.
[7] BILLIET T, VANDENHAUTE M, SCHELFHOUT J, et a1. A review
of trends and limitations in hydrogel-rapid prototyping for tissue
engineering[J]. Biomaterials, 2012, 33(26): 6020-6041.
[8] PEI X, MA L, ZHANG B Q, et al. Creating hierarchical
porosityhydroxyapatite scaffold with osteoinduction by three-
dimensionalprinting and microwave sintering[J]. Biofabrication,
2017, 9(4): 1-24.
图 5 术后 3 个月兔缺损处取材及 H&E 染色形态
Fig. 5 Defect removed and H&E staining at three months [9] LI X (李祥), LI D C (李涤尘), WANG L (王林), et al. Fabrication of
scaffold with controlled porous structure and flow perfusion culture
after surgery of rabbit
in vitro[J]. Chinese Journal of Biotechnology (生物工程学报), 2005,
21(4): 579-583.
3 结论 [10] CUNNIFFE G M, VINARDELL T, MURPHY J M, et al. Porous
decellularized tissue engineered hypertrophic cartilage as a scaffold
通过对病人脊椎缺损的打印可以证明，3D 打印 for largebone defect healing[J]. Acta Biomaterialia, 2015, 23: 82-90.
[11] SATHY B N, WATSON B M, KINARDLA, et al. Bonetissue
技术对不规则形状骨缺损的重建具有一定的可行 engineering with multilayered scaffolds-part Ⅱ: Combining
性，使支架具有与骨缺损部位相吻合的外形。同时 vascularization withbone formation in critical-sized bone defect[J].
Tissue Engineering Part A, 2015, 21(19/20): 2495-2503.
也可以通过打印机针头内径和打印软件设置线条宽
[12] ZHANG L, YANG G, JOHNSON B N, et al. Three-dimensional
度与线条间距，形成支架内部的网线结构。结构可 (3D) printed scaffold and material selection for bone repair[J]. Acta
根据实际情况设定，有利于个性化的根据不同病人 Biomaterialia, 2019, 84: 16-33.
[13] HU C R (胡超然), QIU B (邱冰). 3D bioprinting: applications in
在不同情况下骨缺损制备人工骨支架，为以后临床 cells, scaffolds and bone tissue engineering[J]. Chinese Journal of
使用提供了可行性。 Tissue Engineering Research (中国组织工程研究), 2018, 22(2):
本文只研究了修复时间为 3 个月的打印支架对 316-322.
[14] CASAVOLA C, CAZZATO A, MORAMARCO V. Aorthotropie
骨缺损的治疗情况，其他时间周期还有待进一步探 mechanical properties of fused deposition modelling parts described
索。通过 3 个月的 3D 打印支架修复兔脊椎缺损结 by classicallaminate theory[J]. Materials Design, 2016, 90: 453-458.
[15] WITTBRODT B, PEARCE J M. The effects of PLA color on material
果可以看出，3D 打印支架对骨修复有一定的作用， properties of 3D printed components[J]. Additive Manufacturing,
相比于传统的治疗方法有很大的优势，这也是 3D 2015, 8: 110-116.
打印骨缺损支架的优势。 [16] MENDOZA-BUENROSTRO C, LARA H, RODRIGUEZ C, et a1.
Hybrid fabrication of a 3D printed geometry embedded with PCL
nanofibers fortissue engineering applications[J]. Procedia Engineering,
参考文献：
2015, 110: 128-134.
[1] MOURIÑO V, BOCCACCINI A R. Bone tissue engineering [17] LALONE E A, WILLING R T, SHANNON H L, et a1. Accuracy
therapeutics: Controlled drug delivery in three-dimensionalscaffolds assessment of 3D bone reconstructions using CT: An intro comparison
[J]. Journal of the Royal Society Interface, 2010, 7(43): 209-227. [J]. Medical Engineering & Physics, 2015, 37(8): 729-738.
[2] BEHNIA H, KHOJASTEH A, ESMAEELINEJAD M, et al. Effects [18] DENG W (邓威), ZHENG X (郑欣), RUI M (芮敏), et al. An
of different growth factors on new bone formation: asystem-aticreview experimental study on repairing femoral condyle defect by printing
[J]. Journal of Islamic Dental Association of Iran, 2012, 24(3): porous titanium with 3D technology in rabbits[J]. Laboratory Animal
122-138. and Comparative Medicine (实验动物与比较医学), 2017, 37(4):
[3] OLENDER E, BRUBAKER S, UHRYNOWSKATYSZKIEWICZ I, 266-272.
et al. Autologous osteoblast transplantation, an innovative methodof [19] YUAN Q M (袁秋明). Autologous bone marrow mesenchymal
bone defect treatment: Role of a tissue and cell bank in theproces[J]. stemcells combined with gelatin sponge torepairgoatintervertebral disc
Transplantation Proceedings, 2014, 46(8): 2867-2872. defects[D]. Tianjin: Tianjin Medical University (天津医科大学), 2017.