Page 195 - 《精细化工》2020年第8期

第 8 期 汪洪武，等: 羧基化高纯单壁碳纳米管修饰电极对乙酰甲喹的高灵敏检测 ·1693·

响应情况。结果表明，峰电流分别为70.0、73.6、 2017, 51(2): 19-23.
65.2、64.3 和74.2 μA，相对标准偏差为 6.6%， [4] Commission of Chinese Veterinary Pharmacopoeia (中国兽药典委
员会). Veterinary Pharmacopoeia of the People's Republic of China [M].
说明 cSWCNTs/GE 重现性较好。 Beijing：China Agricultural Press (中国农业出版社), 2014: 104.
2.7 实际样品检测 [5] LIU Q Y, ZHANG J W, LUO X, et al. Further investigations into the
genotoxicity of quinoxaline-di-N-oxides and their primary metabolites[J].
将传感器应用于猪肉样品中乙酰甲喹的检测，
Food & Chemical Toxicology, 2016, 93: 145-157.
结果见表 1。由表 1 可知，加标回收率为 81.7%~ [6] WANG H W, LIU Y Q, YAO S, et al. Fabrication of super pure
124.0%，相对标准偏差 6.9%~12.3%，说明该传感器 singlewalled carbon nanotube electrochemical sensor and its
application for picomole detection of olaquindox[J]. Analytica
能较好地应用于猪肉中乙酰甲喹的高灵敏检测。 Chimica Acta, 2019, 1049: 82-90.
[7] HUANG X J, WANG X, IHSAN A, et al. Interactions of NADPH
表 1 猪肉加标样品检测结果（n=3） oxidase, rennin-angiotensin-aldosterone system and reactive oxygen
Table 1 Results of standard addition of samples species in mequindox-mediated aldosterone secretion in Wistar
rats[J]. Toxicology Letters, 2010, 198(2): 112-118.
加入标准样品浓度/（nmol/L） 回收率/% RSD/% [8] ZHANG J H, GAO H X, PENG B, et al. Simultaneous determination
10 90.7~119.0 12.3 of four synthesized metabolites of mequindox in urine samples using
50 94.2~124.0 10.1 ultrasound-assisted dispersive liquid-liquid microextraction combined
with high-performance liquid chromatography[J]. Talanta, 2012, 88:
100 81.7~102.0 6.9
330-337.
[9] SHAN Q, LIU Y M, HE L M, et al. Metabolism of mequindox and
2.8 机理研究 its metabolites identification in chickens using LC-LTQ-Orbitrap
mass spectrometry[J]. Journal of Chromatography B, 2012, 881/882:
与喹乙醇类似，乙酰甲喹分子结构中也存在
96-106.
1
4
N ,N -二氧环，其中的 N—O 键与苯环相连，容易被 [10] YOU Y L, SONG L T, LI Y S, et al. Simple and fast extraction-
[9]
还原 。故推测乙酰甲喹电化学还原过程为 N—O coupled UPLC-MS/MS method for the determination of mequindox
and its major metabolites in food animal tissues[J]. Journal of
键被还原为 N—H。
Agricultural and Food Chemistry, 2016, 64(11): 2394-2404.
[11] ZHU C Z, YANG G H, LI H. et al. Electrochemical sensors and
biosensors based on nanomaterials and nanostructures[J]. Analytical
Chemistry, 2015, 87(1): 230-249.
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chemical sensors based on two dimensional nanomaterials[J].
Chemical Review, 2019, 119: 478-598.
图 7 乙酰甲喹的电化学还原机理 [13] WANG H W, YAO S, LIU Y Q, et al. Molecularly imprinted
Fig. 7 Conjectural electroreduction mechanism of mequindox electrochemical sensor based on Au nanoparticles in carboxylated
multi-walled carbon nanotubes for sensitive determination of
olaquindox in food and feedstuffs[J]. Biosensors & Bioelectronics,
3 结论 2017, 87: 417-421.
[14] ZHANG Y B, WANG Y, SHI Y F, et al. One step synthesis of
基于 cSWCNTs 修饰电极对乙酰甲喹良好的电 covalent connected three-dimensional graphene/carbon nanotube for
olaquindox electrochemical sensor[J]. Electrochemistry, 2019, 87(1):
催化性能，本研究建立了乙酰甲喹的高灵敏度分析
20-25.
方法，在优化后的检测条件下，1~500 nmol/L 范围 [15] HE W Y, DAI J Y, LI T T, et al. Novel strategy for the investigation
内，修饰电极峰电流与乙酰甲喹浓度之间存在良好 on chirality selection of single-walled carbon nanotubes with DNA
by electrochemical characterization[J]. Analytical Chemistry, 2018,
的线性关系，检出限可达 0.76 nmol/L。利用此法对 90(21): 12810-12814.
猪肉样品进行检测，结果令人满意。相对于其他方 [16] HODGE S A, BAYAZIT M K, COLEMAN K S, et al. Unweaving
the rainbow: A review of the relationship between single-walled
法，本法具有快速、灵敏的特点，利用此法有望实
carbon nanotube molecular structures and their chemical
现实际样品中乙酰甲喹的快速检测。 reactivity[J]. Chemical Society Reviews, 2012, 41(12): 4409-4429.
[17] HOFFERBER E M, STAPLETON J A, IVERSON N M.
参考文献： Review-single walled carbon nanotubes as optical sensors for biological
applications[J]. Journal of the Electrochemical Society, 2020, 167(3):
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+
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