Page 110 - 《精细化工》2021年第10期

·2040· 精细化工 FINE CHEMICALS 第 38 卷

由淡黄色变成橘黄色，具有裸眼识别的效果，于
365 nm 紫外灯照射下，测试溶液的绿色荧光消失。传
2+
感器Ⅰ同 Cu 和 PPi 作用的检测限分别为 5.7×10 –8
–8
和 1.9×10 mol/L，络合物质的量比均为 1∶1。传
2+
感器Ⅰ适用的有效 pH 范围为 5~9，对 Cu 和 PPi
的识别检测至少可实现 4 次循环响应。因此，传感
器Ⅰ是一种能够稳定、高灵敏度和高专一性地检测
2+
Cu 和 PPi 的连续型荧光化学传感器，且有望应用
到生物体内和环境中对离子的识别检测。

图 11 传感器Ⅰ在 V(DMF)∶V(水)=1∶4 的介质中于不 参考文献：
2+
同 pH 下对 Cu 和 PPi 的荧光响应 [1] ANDREWS N C. Metal transporters and disease[J]. Current Opinion
2+
Fig. 11 Fluorescence response of sensorⅠto Cu and PPi at in Chemical Biology, 2002, 6(2): 181-186.
various pH in V(DMF)∶V(water)=1∶4 solution [2] GAGGELLI E, KOZLOWSKI H, VALENSIN D, et al. Copper
homeostasis and neurodegenerative disorders (Alzheimer′s, prion,
2+
2.9 传感器Ⅰ对 Cu 和 PPi 的循环响应测试 and Parkinson′s diseases and amyotrophic lateral sclerosis)[J].
Chemical Reviews, 2006, 106(6): 1995-2044.
本文通过循环响应测试进一步探究了传感器Ⅰ [3] HUNG Y H, BUSH A I, CHERNY R A. Copper in the brain and
2+
对 Cu 和 PPi 识别检测的稳定性，结果见图 12。如 Alzheimer′s disease[J]. Journal of Biological Inorganic Chemistry,
2+
图 12 所示，以 Cu 和 PPi 作为输入信号，以荧光强 [4] 2010, 15(1): 61-76.
LIU K K, ZHANG L N, ZHU L N, et al. A water-soluble, cationic
度作为输出信号，当体系循环响应 4 次之后，荧光 bis-porphyrin exhibiting a more sensitive fluorescent response to
2+
增强数值和荧光猝灭数值之间的差值变化较小，由 Cu relative to its monomeric counterpart[J]. Sensors and Actuators
B: Chemical, 2017, 247: 179-187.
第 1 次的 4362 下降到第 4 次的 4226，整体数值的 [5] TAVALLALI H, DEILAMY G, MOADDELI A, et al. A new
2+
2+
变化趋于平稳，这说明传感器Ⅰ对 Cu 和 PPi 的识 pincer-type “naked-eye” colorimetric probe for Cu determination in
别检测具有较好的稳定性 [55-56] 。 80% water media and its application as a solid state sensor and an
efficient antibacterial product[J]. Sensors and Actuators B: Chemical,
2017, 244: 1121-1128.
[6] BAO Z M, QIN C X, WANG J J, et al. A sensitive and selective
2+
probe for visual detection of Cu based on 1, 8-
naphthalimidederivative[J]. Sensors and Actuators B: Chemical,
2018, 265: 234-241.
[7] RUSSELL R G G. Bisphosphonates: The first 40 years[J]. Bone,
2011, 49(1): 2-19.
[8] LI Y, DONG X H, ZHONG C, et al. A water-soluble two-photon
fluorescent turn-on probe for pyrophosphate anion: Design, synthesis
and properties[J]. Sensors and Actuators B: Chemical, 2013, 183(5):
124-128.
[9] LI F J (李方霁), BIAN Y J (边延江), CHEN Z X (陈志新), et al. A
isophthalic dihydrazide-based fluore scent probe for relay recognition

3+
of Al and pyrophosphate[J]. Chemistry (化学通报), 2019, 82(8):
图 12 传感器Ⅰ于 V(DMF)∶V(水)=1∶4 的介质中交替 743-748.
2+
加入 Cu 和 PPi 之后的循环响应 [10] LIU Z F, ZHU Z L, ZHENG H T, et al. Plasma jet desorption
atomization-atomic fluorescence spectrometry and its application to
Fig. 12 Sequential recognition of sensorⅠupon alternate
2+
addition of Cu and PPi in solution of V(DMF)∶ mercury speciation by coupling with thin layer chromatography[J].
Analytical Chemistry, 2012, 84(23): 10170-10174.
V(water)= 1∶4 [11] RESANO M, ARAMENDI M, BELARRA M A. High-resolution
continuum source graphite furnace atomic absorption spectrometry
for direct analysis of solid samples and complex materials: A tutorial
3 结论 review[J]. Journal of Analytical Atomic Spectrometry, 2014, 29(12):
2229-2250.
合成了一种以 BODIPY 为荧光基团的化学传感 [12] HALL K A, JIANG L W, MARCUS R K. Demonstration of a novel
器Ⅰ，并于 V(DMF)∶V(水)=1∶4 的介质中对 Cu 2+ ion-exchange column for pre-concentration of silver ions in optical
emission spectroscopy utilizing a liquid-sampling atmospheric pressure
呈现出了明显的荧光猝灭响应，荧光猝灭效率可达 glow discharge microplasma[J]. Journal of Analytical Atomic
2+
到 97.7%，同时配合物Ⅰ-Cu 对 PPi 又呈现出了明 Spectrometry, 2017, 32(12): 2463-2468.
[13] CORTE R M, ALVAREZ-FERNANDEZ G R, BLANCO E, et al.
显的荧光增强响应，荧光恢复率可达到 98.5%。传
Quantitative evaluation of cisplatin uptake in sensitive and resistant
2+
感器Ⅰ同 Cu 作用后，于日光灯下，测试溶液颜色 individual cells by single-cell ICP-MS (SC-ICP-MS)[J]. Analytical