Page 153 - 《精细化工》2021年第12期

第 38 卷第 12 期 精 细 化 工 Vol.38, No.12
2021 年 12 月 FINE CHEMICALS Dec. 2021

生物工程
负载白芨多糖的 PU/PAM 双网络水凝胶的制备与性能

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邓孙艳，黄毅萍 ，赵庆仁，胡恩强，鲍俊杰
（安徽大学 化学化工学院，安徽省绿色高分子重点实验室，安徽省水基高分子材料高性能化工程实验室，
安徽 合肥 230601）

摘要：采用聚乙二醇、Ymer N120、聚丙二醇和异佛尔酮二异氰酸酯为原料，以三乙醇胺作交联剂合成聚氨酯
（PU）预聚物，浸入白芨多糖（BSP）和丙烯酰胺（AM）混合溶液，通过自由基聚合制备了负载 BSP 的 PU/PAM
双网络水凝胶。采用 FTIR、SEM 对水凝胶的结构和形貌进行了表征，通过拉力试验机和生物实验对其力学性
能和生物性能进行了测试。结果表明，当三乙醇胺用量为多元醇物质的量的 60%时，双网络水凝胶具有高溶胀
率（256%）的同时保持一定的拉伸强度（1.9 MPa）和高压缩强度（22.7 MPa）。双网络水凝胶具有抗菌抗氧化
作用，其中，双网络水凝胶对大肠杆菌和金黄色葡萄球菌的抑菌带宽度分别为 0.5~4.0 和 0.5~3.5 mm，羟基自由
基清除率最高为 28%；溶血率低于 5%，细胞存活率最高达 101.3%±3.6%，表明双网络水凝胶具有良好的生物相
容性。
关键词：双网络；聚氨酯；丙烯酰胺；白芨多糖；抗菌性能；抗氧化性能；生物工程
中图分类号：TQ427.26；R318.08 文献标识码：A
文章编号：1003-5214 (2021) 12-2515-08 开放科学 (资源服务) 标识码 (OSID)：

Preparation and properties of PU/PAM double network hydrogels
loaded with Bletilla striata polysaccharide

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DENG Sunyan, HUANG Yiping , ZHAO Qingren, HU Enqiang, BAO Junjie
（Key Laboratory of Environment-Friendly Polymer Materials of Anhui Province, Engineering Laboratory of High
Performance Water-Borne Polymer Materials of Anhui Province, College of Chemistry and Chemical Engineering,
Anhui University, Hefei 230601, Anhui, China）

Abstract: A series of polyurethane (PU) prepolymers were prepared using polyethylene glycol, Ymer N120,
polypropylene glycol and isophorone diisocyanate as raw materials and triethanolamine as crosslinker.
Then, the prepolymers were immersed in the mixed solution of Bletilla striata polysaccharide (BSP) and
acrylamide (AM) to prepare BSP-loaded PU/PAM double network hydrogels through free radical
polymerization. The structure and morphology of the hydrogels were characterized by FTIR and SEM. The
mechanical and biological properties of the hydrogels were tested by tensile test machine and biological
experiments. The results showed that when the dosage of triethanolamine was 60% of the amount of
substance of polyols, the prepared double network hydrogel had a high swelling rate (256%) while
maintaining a certain tensile strength (1.9 MPa) and high compressive strength (22.7 MPa). The double-
network hydrogel had antibacterial and antioxidant effects. The antibacterial zone width of E. coli and S.
aureus was 0.5~4.0 and 0.5~3.5 mm, respectively. The scavenging rate of hydroxyl radical was up to 28%.
The rate of hemolysis was less than 5%. And the rate of cell survival was up to 101.3%±3.6%. These results
indicated that the double network hydrogel had good biocompatibility.
Key words: double network; polyurethane; acrylamide; Bletilla striata polysaccharide; antibacterial
properties; antioxidant properties; biological engineering

收稿日期：2021-06-16; 定用日期：2021-08-03; DOI: 10.13550/j.jxhg.20210613
基金项目：安徽省高校科学研究项目（KJ2018ZD004）；安徽省自然科学基金（1808085QE173）
作者简介：邓孙艳（1995—），女，硕士生，E-mail：973385486@qq.com。联系人：黄毅萍（1966—），男，教授，E-mail：yphuang2001@
sina.com。