Page 164 - 《精细化工》2021年第8期

·1658· 精细化工 FINE CHEMICALS 第 38 卷

（微胶囊作用后）可知，微胶囊中释放出的丁香酚 细胞膜上的脂质和蛋白质，从而达到抑菌作用。
破坏大肠杆菌和金黄色葡萄球菌的细胞膜，作用于

图 10 PDA@EO 微胶囊、丁香酚、PDA NPs、EtOH 作用于大肠杆菌（a 1 ~e 1 ）和金黄色葡萄球菌（a 2 ~e 2 ）的荧光照片
及 PDA@EO 微胶囊作用前后大肠杆菌（f 1 , f 2 ）和金黄色葡萄球菌（g 1 , g 2 ）的形貌图
Fig. 10 Fluorescence photos of E. coli (a 1 ~e 1 ) and S. aureus (a 2 ~e 2 ) treated with PDA@EO microencapsules, eugenol, PDA
NPs and EtOH and morphology of E. coli (f 1 , f 2 ) and S. aureus (g 1 , g 2 ) before and after PDA@EO microencapsulation

3 结论 for the treatment of oral mucositis: In vitro and in vivo studies[J].
Controlled Release, 2004, 98(2): 269-279.
[6] HAMID A M S, REHMAN K, CHEN S, et al. Natural and synthetic
（1）以丁香酚作为芯材，DA 作为壳材，采用 polymers as drug carriers for delivery of therapeutic proteins[J].
Polymer Reviews, 2015, 55(3): 371-406.
乳液模板-界面聚合法制备了 PDA@EO 微胶囊。该 [7] WIJETUNGE S S, WEN J, YEH C K, et al. Lectin-conjugated liposomes
as biocompatible, bioadhesive drug carriers for the management of
微胶囊呈规整的球形且尺寸均一，具有明显的核- oral ulcerative lesions[J]. ACS Applied Bio Materials, 2018, 1(5):
壳结构，当 DA 质量浓度为 3.0 g/L，EO 质量浓度 1487-1495.
[8] HOREV B, KLEIN M I, HWANG G, et al. pH-activated nanoparticles
为 6 g/L 时，制备的微胶囊包封率为 22.52%，包封 for controlled topical delivery of farnesol to disrupt oral biofilm
virulence[J]. ACS Nano, 2015, 9(3): 2390-2404.
量为 0.6288 g/g，24 h 时累积释放率最大达到 68.39% [9] YU T J (于陶钧), WANG L B (王立波), WU L J (吴立军). Research
progress of the chemical constituents and pharmacological actions of
（pH 8.0）。 syringa linn[J]. Journal of Anhui Agricultural Science (安徽农业科
（2）制备的 PDA@EO 微胶囊具有非常好的黏 学), 2016, 44(2): 168-170.
[10] GILL A O, HOLLEY R A. Disruption of Escherichia coli, Listeria
附性能和抗冲洗性能，解决了口腔用药生物利用度 monocytogenes and Lactobacillus sakei cellular membranes by plant
oil aromatics[J]. International Journal of Food Microbiology, 2006,
低，黏附性能差及给药频繁的问题。 108(1): 1-9.
（3）PDA@EO 微胶囊对金黄色葡萄球菌和大 [11] DEVI K P, NISHA S A, SAKTHIVEL R, et al. Eugenol (an essential
oil of clove) acts as an antibacterial agent against Salmonella typhi by
肠杆菌的抑菌活性均达到 99%以上，为微胶囊应用 disrupting the cellular membrane[J]. Journal of Ethnopharmacology,
2010, 130(1): 107-115.
于由细菌感染引起的口腔类疾病提供了强有力的理 [12] KATHER M, SKISCHUS M, KANDT P, et al. Functional isoeugenol-
论支撑和实践依据。 modified nanogel coatings for the design of biointerfaces[J].
Angewandte Chemie International Edition, 2017, 56(9): 2497-2502.
[13] DOMARACKY M, REHAK P, JUHAS S, et al. Effects of selected
参考文献： plant essential oils on the growth and development of mouse
preimplantation embryos in vivo[J]. Physiological Research, 2007,
[1] JONES K E, PATEL N G, LEVY M A, et al. Global trends in emerging 56(1): 97.
infectious diseases[J]. Nature, 2008, 451(7181): 990-993. [14] SEBAALY C, JRAIJ A, FESSI H, et al. Preparation and characterization
[2] DEL P J L, PATEL R. The challenge of treating biofilm associated of clove essential oil-loaded liposomes[J]. Food Chemistry, 2015,
bacterial infections[J]. Clinical Pharmacology and Therapeutics, 2007, 178(1): 52-62.
82(2): 204-209. [15] JIN P, YAO R, QIN D K, et al. Enhancement in antibacterial activities
[3] PETCHIAPPAN A, CHATTERJI D. Antibiotic resistance: Current of eugenol-entrapped ethosome nanoparticles via strengthening its
perspectives[J]. ACS Omega, 2017, 2(10): 7400-7409. permeability and sustained release[J]. Journal of Agricultural Food
[4] JOHN C, GUNSOLLE D D S. A meta-analysis of six-month studies Chemistry, 2019, 67: 1371-1380.
of antiplaque and antigingivitis agents[J]. Journal of the American [16] CHONG Y B, ZHANG H, YUE C Y, et al. Fabrication and release
Dental Association, 2006, 137(12): 1649-1657. behavior of microcapsules with double-layered shell containing clove
[5] AKSUNGUR P, SUNGUR A, UNAL S, et al. Chitosan delivery systems oil for antibacterial applications[J]. ACS Applied Materials, 2018,