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·1666· 精细化工 FINE CHEMICALS 第 38 卷

实验结果表明，两种检测方法均显示食品萃取 probes[J]. Organic & Biomolecular Chemistry, 2014, 12(21): 3406-
3412.
液中几乎不含有 H 2 O 2 ；在食品加标实验中，推测因
[12] MA Q J, XU J H, ZHANG X B, et al. A naphthalene-based two-
实验误差以及在实验过程中 H 2 O 2 的分解，致使加标 photon fluorescent probe for selective and sensitive detection of
回收率不同，但对同一样品的测试结果进行比较， thiophenols[J]. Sensors and Actuators B: Chemical, 2016, 229: 434-
440.
荧光检测法对于钛盐比色法的相对误差在 6%以内， [13] MA Q Q, XIAO H B, WANG K, et al. Determination of hydrogen
认为 TC-BOR 可用于检测食品中 H 2 O 2 的含量。 sulfide in endoplasmic reticulum by two-photon fluorescence[J/OL].
Analytical Letters, 2021: 1-13. [2021-02-11]. https://doi.org/10.
1080/00032719.2021.1884255.
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tracer capable of imaging membrane-associated protein tyrosine
本文以半花菁为荧光母体，依据硼酸酯可以被 phosphatase activities[J]. Angewandte Chemie International Edition,
2013, 52(1): 424-428.
H 2 O 2 选择性氧化水解的原理，设计合成了一个长波 [15] XIAO H B, ZHANG Y Z, LI S Z, et al. Spiro-configured fluorescent
2+
长 H 2 O 2 荧光探针 TC-BOR，该探针的特性为： probe: Synthesis and applications in the determinations of Hg and
proton, and two-photon fluorescence imaging[J]. Sensors and Actuators
（1）TC-BOR 在与 H 2 O 2 响应后，在近红外区 B: Chemical, 2016, 236: 233-240.
（723 nm 处）产生强烈的荧光，与加入 H 2 O 2 前相比 [16] YU S L, YANG X L, SHAO Z L, et al. A TICT based two-photon
fluorescent probe for bisulfite anion and its application in living
荧光增强约 55 倍；在 7 min 即可达到最大荧光强度。 cells[J]. Sensors and Actuators B: Chemical, 2016, 235: 362-369.
（2）TC-BOR 具有低细胞毒性以及专一选择性， [17] MENG X M, YE W P, WANG S X, et al. A ratiometric two-photon
fluorescent probe for cysteine and homocysteine in living cells[J].
可用于活体细胞中 H 2 O 2 的识别。
Sensors and Actuators B: Chemical, 2014, 201: 520-525.
（3）将 TC-BOR 用于食品中 H 2 O 2 的检测，结 [18] SUN W, FAN J L, HU C, et al. A two-photon fluorescent probe with
合钛盐比色法进行验证，说明将 TC-BOR 用于食品 near-infrared emission for hydrogen sulfide imaging in biosystems[J].
Chemical Communications, 2013, 49(37): 3890-3892.
中 H 2 O 2 的检测是可行的。 [19] HUANG C B (黄池宝), FAN J L (樊江莉), PENG X J (彭孝军),
基于荧光检测技术开发可用于食品中小分子的 et al. Progress and application of two-photon fluorescent probes[J].
Progress in Chemistry (化学进展), 2007, 19(11): 1806-1812.
快速检测方法，可有效提高食品检测效率与检测精 [20] DU Y C, WANG B W, JIN D, et al. Dual-site fluorescent probe for
−
度，有望成为一种便捷、高效的食品安全检测方法。 multi-response detection of ClO and H 2O 2 and bio-imaging[J].
Analytica Chimica Acta, 2020, 1103: 174-182.
[21] ZHANG J, SHI L, LI Z, et al. Near-infrared fluorescence probe for
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