Page 87 - 《精细化工》2021年第8期

第 8 期 龚孟翔，等: 基于静电纺丝制备的聚乙烯醇/高岭土止血膜 ·1581·

由图 6a 可见，PK0~PK2 不具有细胞毒性。PK0~ [6] LI L L, PENG S J, LEE J K Y, et al. Electrospun hollow nanofibers
for advanced secondary batteries[J]. Nano Energy, 2017, 39:
PK2 的溶血率低于 5%，并不会造成血细胞溶血。由
111-139.
图 6b 可知，溶血率测试实验结束后，测量了培养液 [7] RAMAKRISHNA S, FUJIHARA K, TEO W E, et al. Electrospun
24 h 后的 pH，发现速即纱培养液的 pH 显酸性 nanofibers: Solving global issues[J]. Materials Today, 2006, 9: 40-50.
[8] HUANG Z M, ZHANG Y Z, KOTAKI M, et al. A review on
（pH=3.8），而 PK0~PK2 均显中性（pH=7.0），说明 polymer nanofibers by electrospinning and their applications in
制备的止血膜在使用过程中不会因酸性而引起皮肤 nanocomposites[J]. Composites Science and Technology, 2003, 63:
坏死 [24] 。 2223-2253.
[9] CAVALIERE S, SUBIANTO S, SAVYCH I, et al. Electrospinning:
综合说明，PK0~PK2 止血膜具有良好的生物相 Designed architectures for energy conversion and storage devices[J].
容性，具备潜在的商业化前景。 Energy & Environmental Science, 2011, 4: 761-4785.
[10] NGUYEN L T H, CHEN S L, ELUMALAI N K, et al. Biological,
chemical, and electronic applications of nanofibers[J]. Macromolecular
3 结论 Materials and Engineering, 2013, 298: 822-867.
[11] ZHANG Y, GUAN J, WU J M, et al. N-alkylated chitosan/graphene
（1）利用静电纺丝对不同高岭土含量（基于质 oxide porous sponge for rapid and effective hemostasis in emergency
量分数 10%的 PVA 溶液的质量）的止血膜母液进行 situations[J]. Carbohydrate Polymers, 2019, 219: 405-413.
[12] WANG L Y, YOU X R, DAI C L, et al. Hemostatic nanotechnologies
电喷射打印，制备了具有微观纤维结构的止血膜 for external and internal hemorrhage management[J]. Biomaterials
PK0~PK2。结果表明，静电纺丝技术保证了高岭土 Science, 2020, 8: 4396-4412.
[13] LONG M, ZHANG Y, YANG H M, et al. Emerging nanoclay
有效分布在止血膜中，确保止血膜具有良好的止血
composite for effective hemostasis[J]. Advanced Functional
效果。 Materials, 2018, 28(10): 1704452.
（2）电喷射打印止血膜的止血性能研究发现， [14] BAKER S E, SAWVEL A M, ZHENG N F, et al. Controlling
bioprocesses with inorganic surfaces: Layered clay hemostatic agents[J].
PK2 止血膜在抗凝猪血的凝血时间〔(254 ± 2) s〕和 Chemistry of Materials, 2007, 19(18): 4390-4392.
在 SD 大鼠肝损伤止血模型中的止血性能〔失血量 [15] LIU C Y, YAO W H, QIAO W H, et al. Mussel-inspired degradable
antibacterial polydopamine/silica nanoparticle for rapid
(0.21 ± 0.05) g，止血时间(167 ± 9) s〕，优于空白对
hemostasis[J]. Biomaterials, 2018, 179: 83-95.
照组和商业化止血材料速即纱。 [16] LIU C Y, LIU X, QIAO W H, et al. A highly efficient, in situ
（3）止血膜具有良好的促凝血效果，10 min 可 wet-adhesive dextran derivative sponge for rapid hemostasis[J].
Biomaterials, 2019, 205: 23-37.
实现全血凝血动力学指数降到最低，同时最大可实 [17] ZHAO Z, YAO W H, QIAO W H, et al. Synthesis and evaluation of
现 7.8%的 APTT 降低量和 4.7%的 PT 降低量。 mono- and multi-hydroxyl low toxicity pH-sensitive cationic lipids
for drug delivery[J]. European Journal of Pharmaceutical Sciences,
（4）PK0~PK2 的细胞毒性与溶血率低，生物相
2019, 133: 69-78.
容性好，且制备过程简便、止血性能优良，应用前 [18] JIN N N (靳宁宁), SU L (苏丽), ZHANG J (张静), et al. Alkylated
景较好。 chitosan/polydopamine/grapheme oxide powder for rapid
hemostasis[J]. Fine Chemicals (精细化工), 2020, 37(10): 2009-2014.
[19] THAMER M T, MAYSA M S, AHMED M O, et al. Hemostatic and
参考文献：
antibacterial PVA/kaolin composite sponges loaded with penicillin-
[1] MAISANO F, KJAERGARD H K, BAUERNSCHMITT R, et al. streptomycin for wound dressing applications[J]. Scientific Reports,
Surgical patch versus conventional haemostatic fleece material for 2021, 11: 3428.
control of bleeding incardiovascular surgery: Arandomised controlled [20] LI D J, NIE W, CHEN L, et al. Fabrication of curcumin-loaded
trial[J]. European Journal of Cardio-Thoracic Surgery, 2009, 36(4): mesoporous silica incorporated polyvinyl pyrrolidone nanofibers for
708-714. rapid hemostasis and antibacterial treatment[J]. RSC Advances,
[2] RICKENBACHER A, BREITENSTEIN S, LESURTEL M, et al. 2017, 7: 7973-7982.
Efficacy of Tachosil a fibrin-based haemostat in different fields of [21] LIU C Y, LIU C Y, QIAO W H, et al. Efficient antibacterial
surgery—A systematic review[J]. Expert Opinion on Biological dextran-montmorillonite composite sponge for rapid hemostasis with
Therapy, 2009, 9(7): 897-907. wound healing[J]. International Journal of Biological Macromolecules,
[3] GRANVILLE-CHAPMAN J, JACOBS N, MIDWINTER M. 2020, 160: 1130-1143.
Pre-hospital haemostatic dressings: A systematic review[J]. Injury, [22] ZHENG C, LIU C, QIAO W H, et al. Effective wound dressing
2011, 42: 447-459. based on poly(vinyl alcohol)/dextran-aldehyde composite hydrogel[J].
[4] XI G H, LIU W, CHEN M, et al. Polysaccharide-based lotus seedpod International Journal of Biological Macromolecules, 2019, 132:
surface-like porous microsphere with precise and controllable 1098-1105.
micromorphology for ultrarapid hemostasis[J]. ACS Applied [23] KUMAR V, TAYLOR N L, JALAN A A, et al. A nanostructured
Materials & Interfaces, 2019, 11(50): 46558-46571. synthetic collagen mimic for hemostasis[J]. Biomacromolecules,
[5] YANG X, LIU W, XI G H, et al. Fabricating antimicrobial 2014, 15(4): 1484-1490.
peptide-immobilized starch sponges for hemorrhage control and [24] DAI C L, YUAN Y, LIU C S, et al. Degradable, antibacterial silver
antibacterial treatment[J]. Carbohydrate Polymers, 2019, 222: exchanged mesoporous silica spheres for hemorrhage control[J].
115012. Biomaterials, 2009, 30(29): 5364-5375.