Page 219 - 《精细化工》2022年第12期

第 39 卷第 12 期 精 细 化 工 Vol.39, No.12
2022 年 12 月 FINE CHEMICALS Dec. 2022

精细化工中间体
小麦秸秆预处理对球拟假丝酵母产槐糖脂的影响

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王 彤 ，马晓静 1,2* ，邵君前 ，朱慧霞 1,2 ，王 淮 1,2 ，姚日生 1,2
（1. 合肥工业大学 食品与生物工程学院，安徽 合肥 230009；2. 合肥工业大学 农产品生物化工教育部
工程研究中心，安徽 合肥 230009）

摘要：考察了小麦秸秆的预处理方式对球拟假丝酵母（Starmerella bombicola）利用其糖化液发酵产槐糖脂（SLs）
的影响，并对发酵进行了优化。分别选择稀酸预处理（DAP）、NaOH 预处理（SHP）和 SO 3 微热爆预处理（STMEP）
对小麦秸秆进行预处理，使用纤维素酶酶解糖化后将糖化液用于 SLs 的发酵，考察葡萄糖初始质量浓度和活性
炭脱毒对 SLs 产量的影响。结果显示，SHP 最利于小麦秸秆的酶解糖化，所得糖化液中葡萄糖的初始质量浓度
达 61.30 g/L，其次为 STMEP 和 DAP，葡萄糖的初始质量浓度分别为 48.30 和 40.00 g/L。STMEP 糖化液中抑制
物的总质量浓度最低，其次为 SHP 和 DAP。球拟假丝酵母可以直接利用上述糖化液发酵产 SLs，但发酵特性有
所不同。SHP 和 STMEP 糖化液更利于酸型槐糖脂（ASL）的积累，相比于化学合成培养基，其产量分别提高
了 74.3%和 92.3%，达到(100.450.94)和(110.860.23) g/L。补加葡萄糖和活性炭脱毒可以进一步提高 SLs 的产
量。对于 SHP 糖化液，补加葡萄糖及其与活性炭脱毒的联合可将 ASL 的产量提高至(124.490.15) g/L；对于
STMEP 糖化液，则可将内酯型槐糖脂（LSL）的产量提高至(32.020.11) g/L，与化学合成培养基〔(34.73
0.06) g/L〕的发酵水平相当。
关键词：小麦秸秆；预处理；槐糖脂；球拟假丝酵母；发酵优化；精细化工中间体
中图分类号：TQ920.6 文献标识码：A 文章编号：1003-5214 (2022) 12-2585-08

Influence of pretreatment methods of wheat straw on

sophorolipids production by Starmerella bombicola

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WANG Tong , MA Xiaojing 1,2* , SHAO Junqian , ZHU Huixia , WANG Huai , YAO Risheng
（1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, Anhui, China;
2. Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009,
Anhui, China）
Abstract: The effects of pretreatment methods of wheat straw on sophorolipids (SLs) fermentation by
Starmerella bombicola were investigated, and the fermentation process conditions were then optimized.
Saccharification liquids were produced by cellulase hydrolysis and saccharification of wheat straw, which
were pretreated with dilute acid (DAP), sodium hydroxide (SHP) or sulfur trioxide microthermal explosion
(STMEP) respectively, and then used for SLs fermentation along with glucose supplement and activated
carbon detoxification. The results showed that SHP was the best wheat straw pretreatment in terms of
enzymatic saccharification, with the initial mass concentration of glucose in the saccharification solution
obtained 61.30 g/L, followed by STMEP and DAP with the initial mass concentrations of glucose 48.30 and
40.00 g/L, respectively. Meanwhile, it was found that STMEP saccharification liquid had the lowest mass
concentration of inhibitors, followed by SHP and DAP. Starmerella bombicola could directly use the
saccharification solution obtained above to produce SLs, but with different fermentation characteristics.
Compared with the control chemical synthesis medium, SHP and STMEP saccharification medium were
more conducive to acidic sophorolipid (ASL) accumulation, and the yield increased by 74.3% and 92.3% to

收稿日期：2022-04-08; 定用日期：2022-06-20; DOI: 10.13550/j.jxhg.20220328
基金项目：中央高校基本科研业务费专项资金（JZ2021HGTB0114）；企业委托（W2020JSKF0830，W2021JSKF0443）；安徽省重点研
究与开发计划项目（202004a06020041）；安徽省科技重大专项（202003a05020060）
作者简介：王 彤（1996—），女，硕士生，E-mail：1258422965@qq.com。联系人：马晓静（1985—），女，副教授，E-mail：maxj@hfut.edu.cn。