Page 140 - 《精细化工》2023年第12期

第 40 卷第 12 期 精 细 化 工 Vol.40, No.12
2 023 年 12 月 FINE CHEMICALS Dec. 2023

生物工程
枯草芽孢杆菌发酵甘草渣产 2,3-丁二醇和乙偶姻

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肖志伟 ，周克蒙 ，安雨萌 ，戴建英 ，修志龙 ，党艳艳 ，权春善
（1. 大连理工大学 生物工程学院，辽宁 大连 116024；2. 石河子大学 化学化工学院，新疆 石河子
832003；3. 大连民族大学 生物技术与资源利用教育部重点实验室，辽宁 大连 116600）
摘要：以 2 种稀碱（Na 2 CO 3 水溶液和 NaOH 水溶液，下同）及稀碱和醋酸乙醇胺离子液体混合液为溶剂对甘
草渣进行预处理，考察不同碱浓度和预处理温度对甘草渣组成及酶解效果的影响。结果表明，质量分数为 2%
的 NaOH 水溶液在固液比（g∶mL，下同）为 1∶10、100 ℃条件下预处理甘草渣 1.5 h，木质素去除率达 54.1%、
纤维素回收率为 77.2%；样品酶解 24 h，葡萄糖得率可达 53.5%，较预处理前甘草渣（10.6%）提高了 4.0 倍。
并对预处理后的甘草渣进行高固酶解，在固液比 3∶10、纤维素酶用量 45 FPU/g 甘草渣条件下酶解 72 h，葡萄
糖产量达到 86.2 g/L、木糖产量为 18.9 g/L。以此酶解液为碳源进行发酵，64 h 后发酵液中 2,3-丁二醇和乙偶
姻产量为 43.9 g/L，还原糖转化率为 0.42 g/g；与对照组相比，酶解液更有利于菌体生长，生产强度提高，但
转化率略低。
关键词：甘草渣；预处理；碱溶液；高固酶解发酵；2,3-丁二醇；生物工程
中图分类号：TQ452.1；X705 文献标识码：A 文章编号：1003-5214 (2023) 12-2682-07

Fermentation of 2,3-butanediol and acetoin from

liquorice residue using Bacillus subtilis

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XIAO Zhiwei , ZHOU Kemeng , AN Yumeng , DAI Jianying ,
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XIU Zhilong , DANG Yanyan , QUAN Chunshan
（1. School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China; 2. School of Chemistry
and Chemical Engineering, Shihezi University, Shihezi 832003, Xinjiang, China; 3. Key Laboratory of Biotechnology
and Resource Utilization of Ministry of Education, Dalian Minzu University, Dalian 116600, Liaoning, China）
Abstract: Liquorice residue was pretreated with two dilute basic solutions (Na 2CO 3 aqueous solution and
NaOH aqueous solution) and the mixtures of the above dilute basic solution and ethanolamine acetate,
respectively. The effects of different base concentrations and pretreatment temperature on the composition
and enzymatic hydrolysis of liquorice residue were studied. The results showed that the removal rate of
lignin and cellulose recovery rate reached 54.1% and 77.2%, respectively, under the conditions of 2% (mass
fraction) NaOH aqueous solution as solvent, solid-liquid ratio (g∶mL, the same below) 1∶10, time 1.5 h
and temperature 100 ℃. The glucose yield was 53.5% after 24 h enzymatic digestion, which was 4.0 times
higher than that without pretreatment (10.6%). When the pretreated liquorice residue was subjected to high
solid enzymatic digestion at a solid-liquid ratio of 3∶10 with an enzyme dosage of 45 FPU/g liquorice residue for
72 h, the yield of glucose and xylose reached 86.2 and 18.9 g/L, respectively. This hydrolysate was used as
carbon source for fermentation, the production of 2,3-butanediol and acetoin was 43.9 g/L after 64 h, and
the conversion rate of reducing sugar was 0.42 g/g. Compared with control group, the hydrolysate was more
favorable to the growth of bacteria, while the productivity of 2,3-butanediol and acetoin was increased and
the conversion rate was slightly lower.
Key words: liquorice residue; pretreatment; alkali solution; high-solid enzymatic hydrolysis and fermentation;

收稿日期：2023-03-10; 定用日期：2023-05-29; DOI: 10.13550/j.jxhg.20230194
基金项目：新疆兵团科技攻关计划项目（S2019AB1568）；生物技术与资源利用教育部重点实验室开放课题（KF2022010）
作者简介：肖志伟（1999—），男，硕士生，E-mail：1169210116@qq.com。联系人：戴建英（1972—），女，副教授，E-mail：
jydai@dlut.edu.cn。