Page 62 - 《精细化工》2023年第4期

g748g ㇫㏳ࡃጒ FINE CHEMICALS す 40 ࢤ

䌜㏬ຯఫ 6 ᝭⹧ȡⰥ℁㙵Ⅱ㼐䚣喑L-๖ۙ⅕䚥-Į-㙞 ⣝λ๔㗍ᱳ㣹͚ȡ๔㗍ᱳ㣹Გ⎽⮱ L-๖ۙ⅕䚥-Į-㙞
㓔䚣ڤᰶᰡ倅⮱ ȕ-͆⅕䚥ϔ⢴喑̺ϲڤᰶᲮᑧ⮱Ꮒ 㓔䚣ౕᰭхദڨ᲎У̸喑䚣≨ज䓫 0.19 U/mL喠䚣႓
➖➦ᐯᕔ́ϔ➖ᭀܳ⻨㏜ࡃ喑Ხ๔ౝ䭺ѻγ⩌ϔ ᕔ䉕㶕ᒮ㐀᳉᭫⹧喑ڣᰭ䔯⍖Ꮣহ pH ܳݘͧ 45 ĎȠ
᜽᱙ȡ pH=8.0喑䛾ᆋ⻨ၽᄦڣ䚣≨܍ͻᬍᒞ৺ [31] 喠ౕᰭҠ
⓭≨᲎У̸Ⴂ㗟 22 h喑L-๖ۙ⅕䚥⮱䒙ࡃ⢴㏓ͧ
12 mol/(L·g) [32] ȡᬖ᱌ panD ⮱ᐯ⎽㶕䓫็Გ⎽λ๔
㗍ᱳ㣹喑ѳ䄒Გ⎽⮱ panD ႅౕ䚣≨ѻȠϔ䛼ѻȠ
☚⽠Ⴧᕔጛぶ㑧◦喑̺䔯⩕λ๔㻱὎⮱ጒ͇⩌ϔ喑
䰭ᄦ̺ह㣹⻺Გ⎽⮱ panD 䔈㵹ᰡ⌞ڒ⮱ⵁ⾣ȡₑ
ఫ 6 L-๖ۙ⅕䚥-Į-㙞㓔䚣ݣิ ȕ-͆⅕䚥 ऻ喑ᰡ็⮱ panD ധ఍㷘䞡ჇᎣٸ䮳㶕䓫喑̺ह⩌
Fig. 6 Preparation of ȕ-alanine by L-aspartate-Į-decarboxylase
➖Გ⎽⮱ L-๖ۙ⅕䚥-Į-㙞㓔䚣ࣷᕔ䉕ຯ㶕 6 ᝭⹧ȡ
[6]
Ⱋݺ喑ᄦλ L-๖ۙ⅕䚥-Į-㙞㓔䚣ݣิ ȕ-͆⅕䚥 Გ⎽ͨ㺮ᰶᓛ⩌➖ C. glutamicum [33] ȠB. subtilis Ƞ
⮱ᣏ㉏ͨ㺮ᰶ喟䕇䓴よ䔶̺हᲒ⎽⮱ L-๖ۙ⅕䚥-Į- Corynebacterium crenatum 喍䧊咬᷿ᱳ 㣹喎 [34] Ƞ
㙞㓔䚣㑃⴮ധ఍喍panD喎喑䕇䓴ധ఍ጒ⼸ぶ᝸⃢Ჱᐧ Corynebacterium jeikeium喍Ოℼ᷿ᱳ㣹喎 [35-36] ȠListeria
䒪Ҁ喑ᄳڣᄩڒݝव䔯⮱წͨ㏳㘋͚䔈㵹䛺㏱㶕䓫喠 monocytogenes 喍ࢂᵥ㏳㘋෋ ⩌ᱻ᫜➦㣹 喎 [35] Ƞ
䕇䓴⾮अᩦ䕍㣤ᓄיࡃ᩵⢴ᰡ倅⮱ L-๖ۙ⅕䚥-Į- Lactobacillus plantarum 喍Ḻ➖Πᱳ㣹喎 [7] ࣷ B.
㙞㓔䚣⾮अҀ喠хࡃ䒙ࡃ᲎УᲒ᣽倅䚣ࣺᏁ᩵⢴ぶȡ tequilensis [37] ȠClostridium botuliuum喍㖶⃿ᷚ⟣㟪㘋
L-๖ۙ⅕䚥-Į-㙞㓔䚣⮱㑃⴮ധ఍ panD ᰭᬖࣾ ᱳ㣹喎 [36] ぶ喑Вࣷߕ➖ T. castaneum [38] ぶȡ

㶕 6 ̺ह⩌➖Გ⎽⮱䛻⩌ಸ L-๖ۙ⅕䚥-Į-㙞㓔䚣ࣷᕔ䉕
Table 6 Wild-type L-aspartate-Į-decarboxylase from different biological sources and its properties
Specific Optimum Optimum ȕ-Alanine
Source Host Yield/% References
activity/(U/mg) temperature/ć pH production/(g/L)
E. coli BL21(DE3) E. coli BL21(DE3) 20.15 45 8.0 ÿ ÿ [31]
C. glutamicum E. coli BL21(DE3) 206.50 55 6.0 65.46 97.8 [33]
C. glutamicum E. coli BL21(DE3) 2.60 55 7.0 ÿ ÿ [39]
C. glutamicum E. coli BL21(DE3) 103.00 55 7.0 12.85 ÿ [40]
C. glutamicum E. coli BL21(DE3) 7.52 65 6.5 1.89 ÿ [6]
C. glutamicum E. coli BL21(DE3) 9.60 70 6.5 ÿ ÿ [7]
B. subtilis E. coli BL21(DE3) 8.40 60 6.5 2.23 ÿ [6]
B. subtilis E. coli BL21(DE3) 15.60 65 7.0 3.90 ÿ [7]
T. castaneum E. coli BL21(DE3) 4.83 37 6.5 143.00 80.0 [38]
Lactobacillus plantarum E. coli BL21(DE3) 5.50 70 6.5 ÿ ÿ [7]
Corynebacterium jeikeium E. coli BL21(DE3) 11.80 60 6.0 7.32 82.2 [35]
Listeria monocytogenes E. coli BL21(DE3) 8.90 60 7.0 6.03 67.7 [35]
B. tequilensis E. coli BL21(DE3) ÿ 60 7.5 123.30 99.0 [37]
Clostridium botuliuum E. coli BL21(DE3) ÿ ÿ ÿ 3.62 48.7 [36]
Corynebacterium crenatum Corynebacterium crenatum ÿ ÿ ÿ 0.08 ÿ [34]
∕喟Ąÿą㶕⹧᱗ᰶⰥڠ្䖀ȡ

ᄳ䅤⅕䚥᷿ᱳ㣹Გ⎽⮱ panD ౕ E.coli BL21 10 L ࣾ䚢㑽ⅡᎠ⮱䚣≨䓫ݝγ 684.85 U/mL喑Ꭳ䕇
(DE3)͚䔈㵹ᐯ⎽㶕䓫হ䚣႓ᕔ䉕㶕ᒮ喑㐀᳉᭫⹧喑 䓴ܳឦ㶒᫆᫦ᐼᓄݝγ 65.46 g/L ⮱ ȕ-͆⅕䚥ȡ㮪♣
䄒Გ⎽⮱ L-๖ۙ⅕䚥-Į-㙞㓔䚣℁䚣≨䔉䔉倅λ๔ ᐯ⎽㶕䓫䅤⅕䚥᷿ᱳ㣹Გ⎽⮱ panD ധ఍Ҭᓄ L-๖
㗍ᱳ㣹喑ᰭ䔯⍖Ꮣহ pH ܳݘͧ 55 Ďহ 7.0喑80 Ď ۙ⅕䚥-Į-㙞㓔䚣⮱℁䚣≨㣤ᓄγ䒰๔⮱᣽倅喑ѳ᭜
̸喑䄒䛺㏱䚣⮱ࡷ㶝᱌㏓ͧ 40 min [39] ȡSHEN ぶ [40] ȕ-͆⅕䚥⮱჋䭲ϔ䛼Ꭳ᱗ᓄݝ䒰๔᣽倅喑́ႅౕߍ
䕇䓴 50 mL ᥴ⨣ⅡᎠ⮱㶒᫆ܳឦࣾ䚢喑Ҭᓄౕ ȕ-͆ 䚣䛼䒰๔Ƞ䚣≨̸䭺ȠᏂ➖ើݣぶ䯫В㼐۠⮱䬛䷅喑
⅕䚥ϔ䛼ౕ 36 h ᬣ䓫ݝ 12.85 g/Lȡ䊢䔋ⱌ [33] 䕇䓴ࢂ Ϻ䰭㺮ᄦ䄒䚣䔈̭ₒ⌞ڒⵁ⾣ȡ
఍㉍჋侹хࡃγ䛺㏱㣹⮱䄞ᄩ᲎У喑ౕᰭҠ᲎У̸ ₑใ喑ᄳ L. plantarumȠE. coliȠC. glutamicum