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第 35 卷第 7 期 精 细 化 工 Vol.35, No.7
201 8 年 7 月 FINE CHEMICALS July 2018

中药现代化技术
微波辅助骨碎补黄酮的提取及抗氧化活性研究

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祁小妮 ，王俊龙 ，李振亮 ，党金宁 ，张志琪
（1. 甘肃民族师范学院 化学与生命科学系，甘肃 甘南 747000；2. 陕西师范大学 药用资源与天然药
物化学教育部重点实验室 化学化工学院，陕西 西安 710062）
摘要：采用微波辅助结合响应曲面优化（RSM）法，对骨碎补黄酮提取中的原料质量浓度、微波功率及微波时
间等工艺参数进行了优化，为骨碎补黄酮的定量提取提供了理论依据。考察了骨碎补黄酮清除羟基自由基（OH·）
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和过氧负离子自由基（O 2 ·）的能力。结果表明，骨碎补黄酮的最佳提取条件为：原料质量浓度 0.03 g/L、微波
功率 400 W、微波时间 10 min，在此条件下，骨碎补黄酮的提取率最高，达 5.57%；抗氧化活性实验结果表明，
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当提取的骨碎补黄酮的质量浓度分别为 2.5 和 3.5 g/L 时，骨碎补黄酮对 OH·及 O 2 ·的清除率分别为 10.12%和
35.71%。通过响应曲面确定了提取率与影响因素之间的二次多项函数模型。
关键词：骨碎补；黄酮；微波辅助；抗氧化活性；中药现代化技术
中图分类号：TQ461 文献标识码：A 文章编号：1003-5214 (2018) 07-1170-07

Microwave-assisted Extraction and Antioxidant Activities of
Flavonoids from Drynaria Rhizome

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QI Xiao-ni , WANG Jun-long , LI Zhen-liang , DANG Jin-ning , ZHANG Zhi-qi
（1. Department of Chemistry and Life Sciences, Gansu Normal University for Nationalities, Gannan 747000, Gansu,
China; 2. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education,
School of Chemistry and Chemical Engineering, Shaanxi Normal university, Xi'an 710062, Shaanxi, China）
Abstract: A microwave-assisted extraction procedure of flavonoids from drynaria rhizome was developed
by response surface methodology (RSM) with alcohol as solvent. The effects of mass concentration of raw
material, microwave power and microwave time on the extraction yield of flavonoids were investigated.
The antioxidant activities of the resultant flavonoids were estimated by the scavenging rate against hydroxyl
radical and peroxyl radical. The results showed that the best extraction conditions of flavonoids were
obtained as follows: mass concentration 0.03 g/L, microwave power 400 W, and microwave time 10 min.
Under these extraction conditions, the maximum extraction yield of flavonoids was achieved (5.57%). The
evaluation of antioxidant activity suggested that the flavonoids exhibited significant antioxidant activities.
When the mass concentration of flavonoids was 2.5 g/L, the maximum hydroxy radical scavenging rate was
10.12%, and when the mass concentration of flavonoids reached 3.5 g/L, the maximum scavenging rate for
peroxyl radical was 35.71%. The above experimental results showed that the extraction yield was
determined by RSM and quadratic multinomial function model between influence factors, which could
provide a theoretical basis for the quantitative extraction of flavonoids from drynaria rhizome.
Key words: drynaria rhizome; flavonoids; microwave-assisted; antioxidant activity; modernization
technology of traditional Chinese medicines
Foundation item: Higher Education Institution Research Project of Gansu Province (2016A-093)

收稿日期：2017-04-27; 定用日期：2017-10-26; DOI: 10.13550/j.jxhg.20170358
基金项目：甘肃省高等学校科研项目资助（2016A-093）
作者简介：祁小妮（1987—），女，讲师，硕士，E-mail：xiaoniqiyjs@126.com。联系人：张志琪（1958—），男，教授，博士，E-mail：
zqzhang@snnu.edu。