Page 85 - 《精细化工》2019年第11期

第 11 期 朱 劼，等: 病毒样颗粒限域纳米催化剂提高催化加氢活性 ·2233·

结果显示，催化剂经过 2 次循环使用后，反应 3 h like particles in a flow reactor[J]. Chemical Communications, 2017,
的 CAL 转化率从 77 %降至约 60 %。催化活性出现 53(54): 7632-7634.
了一定程度的下降。这是由于催化剂回收过程较为 [6] Lavelle L, Michel J, Gingery M. The disassembly, reassembly and
stability of CCMV protein capsids [J]. Journal of Virological
繁琐，在此过程造成一些 Ru@CCMV 纳米颗粒的损
Methods, 2007, 146(1/2): 311-316.
失，从而导致其活性的下降。
[7] Willits D, Zhao X, Olson N, et al. Effects of the cowpea chlorotic
mottle bromovirus β-hexamer structure on virion assembly[J].
Virology, 2003, 306(2): 280-288.
[8] Díaz-Valle A, García-Salcedo Y M, Chávez-Calvillo G, et al. Highly
efficient strategy for the heterologous expression and purification of
soluble Cowpea chlorotic mottle virus capsid protein and in vitro
pH-dependent assembly of virus-like particles[J]. Journal of
Virological Methods, 2015, 225: 23-29.
[9] Studier F W, Moffatt B A. Use of bacteriophage T7 RNA polymerase
to direct selective high-level expression of cloned genes[J]. Journal
of Molecular Biology, 1986, 189(1): 113-130.
[10] Ikura K, Kokubu T, Natsuka S, et al. Co-overexpression of folding

modulators improves the solubility of the recombinant guinea pig
图 9 Ru@CCMV 循环使用情况
liver transglutaminase expressed in Escherichia coli[J]. Prep
Fig. 9 Recycling of Ru@CCMV Biochem Biotechnol , 2002, 32(2): 189-205.

[11] Wang Q, Zhao J J, Wang Y, et al. Functional expression of
3 结论 hepassocin in Escherichia coli using SUMO fusion partner and
molecular chaperones[J]. Protein Expression and Purification, 2013,
92(2): 135-140.
成功构建了可高效表达可溶性 CCMV 衣壳蛋
[12] Li Qian, Xu Xiaobin, Chen Huatao, et al. Prokaryotic expression and
白的原核细胞体系，通过优化工艺条件，使可溶性
purification of qinchuan cattle's natural resistance-associated
蛋白表达量达 181 mg/L 发酵液，约占表达蛋白总量 macrophage protein-1 gene (nramp1) N-terminal fragment[J]. Journal
的 93%，并在体外实现对 Ru 纳米颗粒的封装，制 of Agricultural Biotechnology, 2011, 33(5): 195-220.
备出蛋白纳米笼限域催化剂。其在 4-硝基苯酚和肉 [13] Qi Jieqiong (齐洁琼), Gao Hong (高红), Zhuang Tingting (庄婷婷),
桂醛催化加氢反应中表现出较高的催化活性，反应 et al. Expression of recombinant antibody scFv-GCN4 in Escherichia
–1
–1
速率常数分别达到 0.17 min 和 0.50 h ，高于柠檬 coli and determination of its biological activity[J]. Progress in
Modern Biomedicine (现代生物医学进展), 2017, 17(4): 606-609.
酸修饰的 Ru 催化剂。该研究在限域纳米催化剂的
[14] Huang Yanting (黄演婷), Lu Xuemei (卢雪梅), Yang Xiaorong (杨
开发上具有较大应用潜力，为其在可持续化学和绿 小蓉), et al. Renaturation technology of recombinant fusion protein
色催化等领域的应用提供实验基础。 Trx-IFN-CSP[J]. Biotechnology Bulletin (生物技术通报), 2016,
32(6): 219-225.
参考文献： [15] Guo H C, Sun S H, Jin Y, et al. Foot-and-mouth disease virus-like
[1] Wu Dandan (吴丹丹), Zhou Yulong (周玉龙), Ren Yachao (任亚超), particles produced by a SUMO fusion protein system in Escherichia
et al. Construction of colon-targeted nanocarriers based on porcine coli induce potent protective immune responses in guinea pigs, swine
parvovirus-like particles[J]. Microbiology (微生物学通报), 2017, and cattle[J]. Veterinary Research, 2013, 44(1): 48-60.
44(10): 2498-2504. [16] Phelps J P, Dao P, Jin H, et al. Expression and self-assembly of
[2] Leenders S H, Gramage-Doria R, de Bruin B, et al. Transition metal cowpea chlorotic mottle virus-like particles in Pseudomonas
catalysis in confined spaces[J]. Chemical Society Reviews, 2014, fluorescens[J]. Journal of Biotechnology, 2007, 128(2): 290-296.
44(2): 433-448. [17] Zhang Zhenwei (张震威), Le Chuanjun (乐传俊), Hu Jianheng (胡
[3] Liu A, Verwegen M, Maassen S J, et al. Protein cages as containers 建恒), et al. Influence of NbO 2 nano materials in hydrogenation of
for gold nanoparticles[J]. Journal of Physical Chemistry B, 2016, cinnamicaldehyde[J]. Fine Chemicals (精细化工), 2018, 35(8):
120(26): 6352-6357. 1331-1337.
[4] Ueno T, Suzuki M, Goto T, et al. Size-selective olefin hydrogenation [18] Wunder S, Polzer F, Lu Y, et al. Kinetic analysis of catalytic
by a Pd nanocluster provided in an apo-ferritin cage[J]. Angewandte reduction of 4-nitrophenol by metallic nanoparticles immobilized in
Chemie International Edition, 2004, 43(19): 2527-2530. spherical polyelectrolyte brushes[J]. Journal of Physical Chemistry
[5] Liu A, Yang L, Traulsen C H, et al. Immobilization of catalytic virus- C, 2010, 114(19): 8814-8820.