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第 36 卷第 2 期 精 细 化 工 Vol.36, No.2
201 9 年 2 月 FINE CHEMICALS Feb. 2019

中药现代化技术
混合磷酸盐法制备款冬花磷酸酯多糖

赵 鹏，张丽华，王国全，张婷婷

（陕西中医药大学 药学院，陕西 咸阳 712046）

摘要：以款冬花多糖为原料，采用混合磷酸盐法制备款冬花磷酸酯多糖。以磷酸基的取代度（DS）为指标，
在单因素实验的基础上，采用响应面法对款冬花多糖磷酸酯化的合成工艺进行了优化。得到的最优合成工
艺条件为：m〔混合磷酸盐（磷酸二氢钠与磷酸氢二钠）〕∶m（款冬花多糖）=1.5∶1，m（磷酸二氢钠）∶
m（磷酸氢二钠）=2.0∶1，反应时间 6.0 h，反应温度 86 ℃，尿素用量为反应物总质量的 14.25%，在该条
件下制备的款冬花多糖磷酸酯，DS 平均值为 0.697。抗氧化性实验结果表明：款冬花多糖磷酸酯对 DPPH•

（1,1-二苯基-2-苦肼基）最大清除率为 98.91%，O 2 •最大清除率为 48.85%，•OH 最大清除率为 50.34%，均
高于款冬花多糖。
关键词：款冬花；磷酸酯多糖；混合磷酸盐法；合成工艺；抗氧化性；中药现代化技术
中图分类号：TQ28 文献标识码：A 文章编号：1003-5214 (2019) 02-0243-08

Synthesis of Phosphorylated Polysaccharides from
Tussilago farfara by Mixed Phosphate Method

ZHAO Peng, ZHANG Li-hua, WANG Guo-quan, ZHANG Ting-ting
（College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi, China）

Abstract: Phosphorylated polysaccharides from Tussilago farfara were synthesized by mixed phosphate
method. The synthetic process was optimized via response surface method on the basis of single factor
experiment using the substitution degree (DS) of phosphate group as evaluation index. The results showed
that the optimum synthetic conditions were as follows: mass ratio of mixed phosphate to polysaccharides
1.5∶1, mass ratio of sodium dihydrogen phosphate to disodium hydrogen phosphate 2.0∶1,reaction time
6.0 h, reaction temperature 86 ℃, urea dosage 14.25% (based on the total mass of the reactants).Under the
optimized conditions, the average DS of phosphorylated polysaccharides from Tussilago farfara was 0.697. The
results of antioxidant experiment revealed that phosphorylated polysaccharides from Tussilago farfara had

better scavenging abilities on DPPH·, O 2 • and •OH than that of polysaccharides from Tussilago farfara, and

the maximum scavenging rates to DPPH•, O 2 • and •OH were 98.91%, 48.85% and 50.34%, respectively.
Key words: Tussilago farfara; phosphorylated polysaccharides; mixed phosphate method; synthesis
process; antioxidant activity; modernization technology of traditional Chinese medicines
Foundation items: Key Laboratory Project of Education Department of Shaanxi Provincial Government
(14JS024); Shaanxi Province Social Development Science and Technology Project (2016SF-347); Science
Research Project of Shaanxi Provincial Department of Education (14JK1194); Shaanxi University of
Chinese Medicine Fund (14XJZR11)

[1]
多糖广泛存在于动植物体内，具有抗肿瘤 、 性。通过化学合成法对某些多糖的结构进行修饰，
[4]
[5]
调节机体免疫 [2-3] 、降血糖 、抗肝损伤作用 等活 能够改变其生物利用度和生物活性，这是深化对糖

收稿日期：2018-05-30; 定用日期：2018-11-08; DOI: 10.13550/j.jxhg.20180391
基金项目：陕西省教育厅重点实验室项目（14JS024）；陕西省社会发展科技攻关项目（2016SF-347）；陕西省教育厅专项资助项目
（14JK1194）；陕西中医药大学校内基金（14XJZR11）
作者简介：赵 鹏（1977—），男，教授，博士，E-mail：zhaopeng65@sina.com。