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·1110· 精细化工 FINE CHEMICALS 第 36 卷

由表 2 可知，巨尾桉树皮和树叶中单宁提取物 参考文献：
对实验所选供试菌均有较强的抑制作用，经大孔树 [1] Tian Yuhong (田玉红), Liu Xiongmin (刘雄民), Tao Mingyou (陶明
脂纯化后的树皮及树叶单宁纯化物对 5 种细菌的抑 有). Extraction and determination of volatile constituents of leaves
from E. grandis x E. urophylla[J]. Journal of Guangxi Academy of
菌效果明显优于粗提物的抑菌效果。树皮单宁纯化 Sciences (广西科学院学报), 2006, 22(S1): 466-468.
物对大肠杆菌、金黄色葡萄球菌、痢疾杆菌、沙门 [2] Hou A J, Liu Y Z, Yang H, et al. Hydrolyzable tannins and related
polyohenols from Eucalyptus globules [J]. Journal of Asian Natural
氏菌、蜡状芽孢杆菌的抑菌效果比粗提物的抑菌效
Products Research, 2000, 2(3): 205-212.
果分别提高了 18.61%、19.39%、16.13%、36.91%、 [3] Zhan Nianyong (湛年勇), Yao Chuangcheng (姚创程), Lu Wenhai
38.77%；树叶单宁纯化物对 5 种供试菌的抑菌效果 (陆文海), et al. Analysis of tannins of Eucalyptus bark [J]. Biomass
Chemical Engineering (生物质化学工程), 2009, 43(5): 39-41.
比粗提物的抑菌效果分别提高了 0.53%、17.13%、 [4] Huang Zeng (黄增), Liu Xiongmin (刘雄民), Ku Yongfeng (库咏
8.70%、44.77%、28.05%。此外，树皮单宁纯化物 峰), et al. Extraction and separation of tannins from Eucalyptus
Grandis x E. urophylla and its antimicrobial and antioxidant activity
对大肠杆菌、沙门氏菌以及蜡状芽孢杆菌的抑制能 [J]. Fine Chemicals (精细化工), 2012, 29(6): 564-567.
力强于阿莫西林。对巨尾桉单宁纯化物而言，其抑 [5] Zhou Zhou (周洲), Long Fengxiang (龙凤香), Deng Lijuan (邓黎
娟), et al. Extraction，antioxidant activity of tannins from different
菌圈直径均在 10 mm 以上，其中对痢疾杆菌的抑菌
parts of Eucalyptus Grandis x E. Urophylla[J]. Science and
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达 13.25、12.50 mm。阳性对照品对蜡状芽孢杆菌的 129-132.
[6] Huang Zeng (黄增), Liu Xiongmin (刘雄民), Huang Likui (黄丽葵),
抑菌圈直径仅为 7.64 mm，树皮单宁纯化物、树皮 et al. Extraction，separation and antioxidant activity of saponins from
单宁粗提物、树叶单宁纯化物和树叶单宁粗提物对 Eucalyptus grandis x E. urophylla[J]. Natural Product Research
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其抑菌作用均强于阳性对照。
[7] Zheng Yanfei (郑燕菲), Liu Xiongmin (刘雄民), Guo Zhanjing (郭
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3 结论 of total flavonoids from leaves of Eucalyptus Grandis x E.
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[8] Yue Xinyin (岳芯吟), Liu Xiongmin (刘雄民), Ma Li (马丽), et al. Study
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佳静态纯化条件为：树脂与样液固液比为 200︰1 from leaves of Eucalyptus Grandis x E. Urophylla[J]. Applied
Chemical Industry (应用化工), 2015, 44(1): 15-19.
（g/mL）、样液质量浓度 2.0 g/L、吸附时间 75 min、 [9] Lv Chenglin (吕成林), Wang Qiukuan (汪秋宽), Song Yuefan (宋悦
解吸液乙醇体积分数 80%；动态纯化条件为：上样 凡), et al. Study on the extraction and purification of polyphenols
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3 BV，该条件下纯化物中单宁的质量分数为 47.82%。 [10] Zhang Yiming (张一鸣), Wu Yuezhong (吴跃中), Yang Shihua (杨士
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时间 120 min、解吸液乙醇体积分数 80%，动态纯化 [11] Ku Yongfeng (库咏峰) . Study on extraction and separation analysis
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吸液用量 3 BV，该条件下纯化物中单宁的质量分数 [12] Dong Xiao ( 董晓 ), Jiang Zitao ( 姜子涛 ), Li Rong ( 李荣).
为 37.00%。 Antioxidant activities of Dill leaf essential oil [J]. Food Science and
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（2）巨尾桉树皮和树叶中单宁对 DPPH•和•OH [13] Liu Jun (刘俊). Research on a new method of determination and
有一定的清除能力。PB、PL、CB、CL 对 DPPH• elimination of free radical [J]. Journal of Wuhan Institute of
Technology (武汉工业学院学报), 2005, 24(2): 53-55.
清除作用的 IC 50 值分别为 0.0121、0.0168、0.0280、
[14] Shen Kai (申凯), Zhang Hui (张辉), Yan Biyun (严碧云), et al.
0.0281 g/L；各试样对•OH 的清除效果依次为：PB> Study on extraction of polyphenols from stems of TrapabispinosaRoxb.
PL>CL>CB>V C 。 and its antioxidant and antimicrobial activities [J]. Science and
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菌有较强的抑制作用。树皮和树叶单宁纯化物的抑 [15] Lin X Q, Wu J L, Jin X H, et al. Selective separation of biobutanol
from acetone–butanol–ethanol fermentation broth by means of
菌性比粗提物的抑菌性强，且对痢疾杆菌的抑菌圈
sorption methodology based on a novel macroporous resin[J].
直径分别达 13.25、12.50 mm。单宁纯化物和粗提物 Biotechnology Progress, 2012, 28(4): 962-972.
对蜡状芽孢杆菌的抑菌性均强于阿莫西林溶液。 [16] Liu Xiaoli (刘晓丽), Wu Kegang (吴克刚), Chai Xianghua (柴向华),
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[17] Zheng Yanfei ( 郑燕菲 ). Study on effective component and
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