Page 146 - 201906

·1152· 精细化工 FINE CHEMICALS 第 36 卷

of IL-6, IL-1β and TNF-α were determined using 3.2 Adsorption kinetics
ELISA kits as per the manufacturer’s instructions. The adsorption kinetics curves for FMTs on AB-8
2.10 Statistical analysis resin were obtained at 25 ℃ and are shown in Fig.2A.
All the data are presented as the mean ± standard The FMTs adsorption capacities rapidly increased with
deviation (SD). One-way analysis of variance (ANOVA)
and Student’s-test were performed to evaluate the
significant differences. Statistical significance was
considered when the P value was less than 0.05 (P＜
0.05). All statistical analyses were performed by using
SPSS 18.0 software.

3. Results and Discussion

3.1 Selection of macroporous resins suitable for
the separation of FMTs
Macroporous resins can be used for the adsorption
of organic constituents due to their hydrophobic
properties. The adsorption capacity of macroporous
resins correlates not only with the physical and
chemical properties of the adsorbent but also with the
size and chemical features of the adsorbed substance.
To effectively enrich flavonoids, the optimum type of
macroporous resin was screened. First, the properties
of twelve resins were compared in terms of their
adsorption and desorption capacities. As shown in
Fig.1, among the selected resins, the adsorption
capacity of AB-8 resin to FMTs was 105.89 mg/g and
the desorption ratio of AB-8 resin was 86.31%. All of
the above indicators were higher than those of the
others, and NKA-9 resin showed the lowest adsorption
capacity, which indicated that differences existed in
the adsorption capacities for different resins. This
result indicated that an appropriate surface area and
resin size were both crucial for total purification.
Taking the adsorption and desorption ratios into
account, AB-8 resin was considered the most
appropriate resin for FMTs purification among the
tested resins.

Fig. 2 Adsorption kinetics curves for FMTs on AB-8 resin
at 25 ℃ (A); adsorption isotherms for FMTs on AB-8

resin at different temperatures (25 ℃, 35 ℃ and
Fig. 1 Static adsorption and desorption results based on 45 ℃) (B); effects of the initial concentration of the
the phloridzin content in different types of sample solution on the adsorption capacities and
macroporous resins. The results were expressed as adsorption ratios of FMTs on AB-8 resin (C); effects
mean ± S.D. (n = 3). The mean values were of the pH value on FMTs adsorption capacities of the
considered significantly different when P<0.05. AB-8 resin (D).