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第 36 卷第 6 期 精 细 化 工 Vol.36, No.6
201 9 年 6 月 FINE CHEMICALS June 2019

医药与日化原料

Separation and Purification of Flavonoids in Malus toringoides
(Rehd.) Hughes Leaves with Macroporous Resins and

Evaluation of anti-Inflammation Activities

1
2
1*
2
1
CHANG Bing-quan , LI Bin , YUAN Rui-ying , ZHUOMA Dong-zhi , HUANG shan
(1. Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology,
Qingdao 266042,Shandong China; 2. Department of Medicament, College of Medicine, Tibet University, Lhasa 850000,
Xizang,China)
AR TICLE INFO A B S T R A C T

Article history: The purpose of this research was to study the purification process of flavonoids in Malus
Received 29 October 2018 toringoides (Rehd.) Hughes leaves (FMTs), adsorption and desorptiontests optimised the
Accepted 29 January 2019 purification parameters. AB-8 resin was selected by comparing the adsorption and
Published 15 june 2019 desorption ability of FMTs on twelve types of resins. The results showed that the optimal
DOI:10.13550/j.jxhg.20180797 purification parameters of static adsorption and desorption were the sample at pH=4 with 3

h of adsorption; the desorption solvent was 70% ethanol (volume fraction, pH=7.0) with 3 h
Keywords: of desorption. The best dynamic parameters were a sample loading amount of 9 bed
Malus toringoides (Rehd.) Hughes volumes (BV) at a feeding flow rate of 2 BV/h and washing the column with 6 BV of water,
leaves followed by subsequent flushing with 20% (6 BV), 40% (6 BV), 60% (6 BV) and 80%
Flavonoids (6 BV) ethanol in water (volume fraction) at an elution flow rate of 2 BV/h. Under these
Macroporous resins conditions, the mass fraction of FMTs increased from 32.79% to 59.18%. The existence of
Anti-inflammation activities flavonoids was confirmed by their characteristic functional groups that were identified by
Drug and cosmetic materials infrared spectroscopy. Meanwhile, FMTs in the dosage range of 50 to 400 mg/L showed a
significant anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW 264.7
mouse macrophage cells, which could inhibit the secretion of NO, IL-6, IL-1β and TNF-α
Foundation item: through the inhibition of inflammatory-related gene expression.
National Natural Science
Foundation of China (81760779);
Natural Science Foundation of Article ID: 1003-5214(2019)06-1149-10
Xizang (Tibet) Autonomous Region
(XZ2017ZRG-07)


1. Introduction biological and pharmacological activities, including
anti-allergic, anti-inflammatory, antioxidant, anti-
As a traditional folk medicine in Tibet, Malus microbial, anti-cancer and anti-diarrheal activities [5-10] .
toringoides (Rehd.) Hughes (MT), a rosaceous plant, Previous studies have shown that flavonoids from the
is widely distributed on the Qinghai-Tibetan Plateau Malus toringoides (Rehd.) Hughes leaves may possess
and can be obtained at an altitude of 3000-3700 m [1-2] . an antidiabetic activity in diabetic mice and rats [11] .
Fresh MT leaves are usually used as a healthy drink in Hence, it is necessary to develop an effective
Tibet. Among Tibetan people, this drink is regarded as purification technology to obtain FMTs with high
a medicine to treat diseases such as liver injury, purity for pharmaceutical applications.
hypopepsia, hyperglycemia, hyperlipidemia and Recently, sorbents, particularly macroporous resins,
hypertension [3-4] . Flavonoids, widely distributed in have been widely applied in the separation and
plants, have been shown to have a wide range of enrichment of active ingredients in natural products.
Macroporous resins display distinguishing features
First author: Chang Bingquan (1993—), male, master degree candidate, such as procedural simplicity, high purification
E-mail: changbingquan@126.com. efficiency, good stability, and easy regeneration [12-14] .
Corresponding author: Huang Shan (1975—), male, doctor, associate Macroporous resins can selectively adsorb targeted
professor, E-mail: huangshan@qust.edu.cn.