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·1158·                            精细化工   FINE CHEMICALS                                  第 36 卷





                    变叶海棠叶总黄酮的分离纯化及体外抗炎活性



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                            常柄权 ，李   斌 ，袁瑞瑛 ，卓玛东智 ，黄   山
                    （1.  青岛科技大学  化工学院，山东  青岛    266042；2.  西藏大学  医学院，西藏  拉萨    850000）
                 摘要：通过吸附和解吸附实验，考察并优化了变叶海棠叶中总黄酮的纯化工艺及工艺参数。通过比较 12 种不同
                 极性的大孔吸附树脂对变叶海棠叶总黄酮的吸附、解吸附能力，最终选取 AB-8 树脂进行实验。结果表明，最
                 优静态吸附、解吸附工艺参数为：pH=4 下静态吸附 3  h；体积分数为 70%的乙醇（pH=7）静态解吸附 3  h；最
                 优动态吸附、解吸附工艺参数：以 2 BV/h 的流速上样 9 BV（柱体积）样品溶液，随后在 2 BV/h 流速下，分别
                 以 6 BV 水、6 BV 体积分数为 20%的乙醇、6 BV 体积分数为 40%的乙醇、6 BV 体积分数为 60%的乙醇、6 BV
                 体积分数 80%的乙醇对上样柱进行洗脱，在此条件下分离纯化，变叶海棠叶总黄酮质量分数由 32.79%提高到
                 59.18%。红外光谱显示变叶海棠叶中具备黄酮类物质特征官能团，证明黄酮类物质的存在。对精制后的变叶海
                 棠叶总黄酮进行体外抗炎作用考察，在 50~400  mg/L 质量浓度下，变叶海棠叶总黄酮对脂多糖诱导巨噬细胞炎
                 症反应有明显的抑制作用，可能是通过抑制与炎症相关的基因表达来抑制 NO、IL-6、IL-1β、TNF-α 的分泌。
                 关键词：变叶海棠叶；黄酮；大孔吸附树脂；抗炎活性;  医药与日化原料
                 中图分类号：TQ 914.1      文献标识码：A
                 基金项目：国家自然科学基金（81760779）；西藏自治区自然科学基金（XZ2017ZRG-07）




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