Page 83 - 精细化工2019年第8期

第 36 卷第 8 期 精 细 化 工 Vol.36, No.8
201 9 年 8 月 FINE CHEMICALS Aug. 2019

中药现代化技术
柠檬桉树脂总黄酮的提取纯化及抗氧化活性

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李丽丽，孙泽宇，刘雄民 ，于 唱，卢贤锐，王一凡
（广西大学 化学化工学院，广西 南宁 530004）

摘要：采用溶剂法提取纯化柠檬桉树脂总黄酮，对粗提物（A）和纯化物进行抗氧化活性研究。用盐酸-锌粉、
浓硫酸、三氯化铁、硝酸铝等方法定性鉴别树脂总黄酮，以芦丁为标准品定量分析树脂总黄酮含量为 4.64%（以
柠檬桉树脂固体粉末的质量为基准，下同）。粗提物（A）经氯仿-乙酸丁酯、氯仿-二甲基甲酰胺、二甲基甲酰
胺-氯仿两次超声萃取后得到纯化物 B、C、D 中总黄酮质量分数分别为 10.39%、18.03%、44.15%。考察了 A、
B、C、D 对 2,2-二苯基-1-苦味基肼自由基（DPPH·）的清除活性、总抗氧化能力和还原能力。结果表明，质量
浓度为 0.14 g/L 时，A、B、C、D 对 DPPH·的清除活性分别为 91.75%、74.22%、88.44%、91.56%；总抗氧化能
力分别为 19.99、1.75、4.39、3.79 μmol 芦丁当量（RE）/g；还原能力分别为 1.16、0.36、0.39、0.45 μmol RE/g。
关键词：柠檬桉；树脂；总黄酮；萃取纯化；抗氧化活性；中药现代化技术
中图分类号：TQ28 文献标识码：A 文章编号：1003-5214 (2019) 08-1571-07

Extraction, Purification and Antioxidant Activities of
Total Flavonoids in Eucalyptus citriodira Resin

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LI Li-li, SUN Ze-yu, LIU Xiong-min , YU Chang, LU Xian-rui, WANG Yi-fan
（College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China）

Abstract: Total flavonoids in Eucalyptus citriodiraresin were extracted and purified by solvent method and
antioxidant activities of crude extract (A) and purified compounds were studied. The total flavonoids in
resin were qualitatively identified by hydrochloric acid-zinc powder, concentrated sulfuric acid, ferric
chloride and aluminum nitrate.Total flavonoids content (based on the quality of Eucalyptus citriodira resin
solid powder, the same below) was 4.64% by quantitative analysis with rutin as standard substance.The
purified compounds B, C and D were obtained by ultrasonic extraction of the crude extract (A) using
chloroform and butyl acetate, chloroform and dimethyl formamide, dimethyl formamide and chloroform as
extract solvent. Their corresponding mass fraction of total flavonoids were 10.39%, 18.03% and 44.15%.
The scavenging activity, total antioxidant capacity and reducing power of A, B, C and D against 2,
2-diphenyl-1-bitter hydrazine radical (DPPH) were investigated. When mass concentration was 0.14 g/L,
The scavenging activity of A, B, C and D on DPPH· were 91.75%, 74.22%, 88.44% and 91.56% ,
respectively. The total antioxidant capacity was 19.99, 1.75, 4.39 and 3.79 μmol rutin equivalent (RE)/g.
The reducing power was 1.16, 0.36, 0.39 and 0.45 μmol RE/g.
Key words: Eucalyptus citriodira; resin; total flavonoids; extraction and purification; antioxidant activity;
modernization technology of traditional Chinese medicines

柠檬桉（Eucalyptus citriodira）为桃金娘科常绿 一种黑褐色胶性脂液。刚流出来呈液态，长期暴露
大乔木，在中国主要分布于广西、福建、广东、海 在空气中则为硬脂。其味苦涩辛，性微温，有收敛、
[1]
南、云南、四川、江西、湖南等省（区）。柠檬桉树 清热、解毒、杀菌的作用 。有关柠檬桉枝叶、果
脂是柠檬桉受伤的树干或干裂的树皮创口里流出的 实的挥发油、多酚等成分提取分离及活性研究已有

收稿日期：2018-09-21; 定用日期：2019-03-08; DOI: 10.13550/j.jxhg.20180706
基金项目：国家自然科学基金（11762003）
作者简介：李丽丽（1993—），女，硕士生。联系人：刘雄民（1960—），男，教授，博士生导师，E-mail：xmliu1@gxu.edu.cn。