Page 98 - 精细化工2019年第9期
P. 98
·1826· 精细化工 FINE CHEMICALS 第 36 卷
V max 为 3.9 mmol/h,其 K m 小于包衣菌体,表明经包 Science and Technology of Cereals, Oils and Foods (粮油食品科技),
衣处理后,菌体中亚油酸异构酶和底物的亲和力有 2003, 11(4): 4-7.
[8] Jung M, Jung M. Identification of conjugated linoleic acids in
所降低。 hydrogenated soybean oil by silver ion-impregnated HPLC and gas
chromatography-ion impacted mass spectrometry of their 4,
3 结论 4-dimethyloxazoline derivatives[J]. Journal of Agricultural and Food
Chemistry, 2002, 50(21): 6188-6193.
[9] Kishino S, Ogawa J, Yokozeki K, et al. Metabolic diversity in
(1)以非离子表面活性剂谷氨酸双十二烷基酯
biohydrogenation of polyunsaturated fatty acids by lactic acid
核糖醇(2C 12 GE)对 Lactobacillus helveticus L7 进 bacteria involving conjugated fatty acid production[J]. Applied
行包衣处理,包衣菌体中的亚油酸异构酶在有机溶 Microbiology and Biotechnology, 2009, 84(1): 87-97.
[10] Wallace R, Mckain N, Shingfield K, et al. Isomers of conjugated
剂中具有催化合成 c9,t11-CLA 的活性,催化 18 h linoleic acids are synthesized via different mechanisms in ruminal
时, c9,t11-CLA 的产量为 1906 mg/L ,转化率 digesta and bacteria[J]. Journal of Lipid Research, 2007, 48(10):
为 95.3%,包衣菌体在有机溶剂中可重复使用 5 次 2247-2254.
[11] Liu X, Li H, Chen Y, et al. Method for screening of bacterial strains
以上。 biosynthesizing specific conjugated linoleic acid isomers[J]. Journal
(2)表面活性剂的种类、添加量和溶剂种类、 of Agricultural and Food Chemistry, 2012, 60(38): 9705- 9710.
[12] Yong Y, Bai Y, Li Y, et al. Characterization of Candida rugosa lipase
缓冲液 pH、包衣时间、包衣温度和干燥方式均会影
immobilized onto magnetic microspheres with hydrophilicity[J].
响包衣菌体的酶活。最适包衣条件为:将 2C 12 GE Process Biochemistry, 2008, 43(11): 1179-1185.
配制成质量分数为 1.0%的丙酮溶液,菌体用 pH 5.8 [13] Thakar A, Madamwar D. Enhanced ethyl butyrate production by
surfactant coated lipase immobilized on silica[J]. Process
的磷酸盐缓冲液重悬,4 ℃下处理 4 h,冷冻干燥而
Biochemistry, 2005, 40(10): 3263-3266.
成包衣菌体。在以亚油酸为底物时包衣菌体中亚油 [14] Wu J, Song B, Xing A, et al. Esterification reactions catalyzed by
酸异构酶的 K m 为 35.7 mmol/L,V max 为 5.6 mmol/h。 surfactant-coated Candida rugosa lipase in organic solvents[J].
Process Biochemistry, 2002, 37(11): 1229-1233.
(3)经包衣处理后,菌体中亚油酸异构酶和底物 [15] Iram M, Ishfaq A, Guo C, et al. A surfactant-coated lipase
的亲和力有所降低。包衣菌体在有机介质中的催化机 immobilized in magnetic nanoparticles for multicycle ethyl
isovalerate enzymatic production[J]. Biochemical Engineering
理还有待更深入研究。
Journal, 2013, 73(8): 72-79.
[16] Song Baodong (宋宝东), Xing Aihua (邢爱华), Wu Jinchuan (吴金
参考文献: 川), et al. Synthesis and application of glutamate dialkyl riboalcohol[J].
[1] Larkin A, Lacouture M, Boutsalis G, et al. Impact of continuing Petrochemical Engineering (石油化工), 2001, 30(S): 376-378.
medical education on the treatment of hypertriglyceridemia with [17] Jin Lei (金磊), Liu Ping (刘萍), Li Haixing (李海星), et al.
omega-3 fatty acids[J]. Circulation: Cardiovascular Quality and Characteristic analysis of rich conjugated linoleic acid yogurt[J].
Outcomes, 2016, 9(S2): A168. Food and Fermentation Industries (食品与发酵工业), 2015, 41(6):
[2] Rodríguez-Castañedas J, Pena-Egido M. Quantitative determination 76-80.
of conjugated linoleic acid isomers by silver ion HPLC in ewe milk [18] Yoshio O, Kuniharu I. A lipid-coated lipase as a new catalyst for
triglyceride synthesis in organic solvents[J]. Journal of the Chemical
fat[J]. Journal of Food Composition and Analysis, 2011, 24(7):
1004-1008. Society Chemical Communications, 1988, 20(20): 1392-1394.
[19] Liu Xiaohua (刘晓华), Chen Yan (陈燕), Li Haixing (李海星), et al.
[3] Kim K, Lee J, Park Y, et al. ATF3 mediates anti-cancer activity of
trans-10, cis-12-conjugated linoleic acid in human colon cancer Bioconversion of conjugated linoleic acid by washed cells of
Lactobacillus helveticus L7[J]. Food Science and Technology (食品
cells[J]. Biomolecules & Therapeutics, 2015, 23(2): 134-140. 科技), 2011, 36(8): 8-11.
[4] Aydın B, Şekeroğlu Z, Şekeroğlu V, et al. Effects of whey protein
[20] Ding Hui (丁辉), Song Baodong (宋宝东), Wu Jinchuan (吴金川), et
and conjugated linoleic acid on acrolein-induced cardiac oxidative al. Surfactant-coated lipase as a catalyst for hydrolytic reaction of
stress, mitochondrial dysfunction and dyslipidemia in rats[J].
olive oil in organic/aqueous two-phase system[J]. Journal of Tianjin
Biomedicine & Pharmacotherapy, 2018, 107 (11): 901-907. University (天津大学学报), 2004, 37(8): 663-666.
[5] Baraldi F, Vicentini T, Teodoro B, et al. The combination of [21] Yang Gensheng (杨根生), Wu Weijun (吴伟军), Ou Zhimin (欧志
conjugated linoleic acid (CLA) and extra virgin olive oil increases 敏 ), et al. Preparation of S-(+)-ibuprofen by lipocaptase in
mitochondrial and body metabolism and prevents CLA-associated water-organic solvent mixed system[J]. Chemical Engineering (化学
insulin resistance and liver hypertrophy in C57Bl/6 mice[J]. Journal 工程), 2010, 38(1): 94-98.
of Nutritional Biochemistry, 2016, 28: 147-154. [22] Grabner B, Nazario M, Gundersen M, et al. Room-temperature solid
[6] Mądry E, Chudzicka-Strugala I, Grabańska-Martyńska K, et al. phase ionic liquid (RTSPIL) coated ω-transaminases: Development
Twelve weeks CLA supplementation decreases the hip circumference and application in organic solvents[J]. Molecular Catalysis, 2018,
in overweight and obese women. A double-blind, randomized, 452(6): 11-19.
placebo-controlled trial[J]. Acta Scientiarum Polonorum Technologia [23] Ishiguro Y, Furugen A, Narumi K, et al. Valproic acid transport in the
Alimentaria, 2016, 15(1): 107-113. choriocarcinoma placenta cell line JEG-3 proceeds independently of
[7] Ling Li (凌利), Zhang Dali (章达礼), Xu Yan (徐焱), et al. Research the proton-dependent transporters MCT1 and MCT4[J]. Drug
on the test of food safety toxicology of conjugate linoleic acid[J]. Metabolism and Pharmacokinetics, 2018, 33(6): 270-274.